Recreating a human trabecular meshwork outflow system on microfabricated porous structures

Biotechnology and Bioengineering

Volumn 110, Issue 12, 2013, Pages 3205-3218

  Torrejon, Karen Y ^a   Pu, Dennis ^a   Bergkvist, Magnus ^a   Danias, John ^b   Sharfstein, Susan T ^a   Xie, Yubing ^a  

^a STATE UNIVERSITY OF NEW YORK   (United States)

^b SUNY DOWNSTATE MEDICAL CENTER   (United States)

Author keywords

Glaucoma; Intraocular pressure; Outflow; Porous; SU 8; Trabecular meshwork

Indexed keywords

GLAUCOMA; INTRA OCULAR PRESSURE; OUTFLOW; POROUS; SU-8; TRABECULAR MESHWORKS;

CYTOLOGY; MORPHOLOGY; OPHTHALMOLOGY; PHYSIOLOGY; PROTEINS; SENSORS;

SCAFFOLDS (BIOLOGY);

ALPHA CRYSTALLIN; F ACTIN; LATRUNCULIN B; TISSUE SCAFFOLD;

ARTICLE; CELL CULTURE; CELL DENSITY; CONTROLLED STUDY; CYTOLOGY; GLAUCOMA; HUMAN; HUMAN CELL; HUMAN TRABECULAR MESHWORK; IMAGE ANALYSIS; IMMUNOCYTOCHEMISTRY; IN VITRO STUDY; INTRAOCULAR PRESSURE; NANOFABRICATION; RISK FACTOR; SCANNING ELECTRON MICROSCOPY; TRABECULAR MESHWORK;

GLAUCOMA; INTRAOCULAR PRESSURE; OUTFLOW; POROUS; SU-8; TRABECULAR MESHWORK;

GLAUCOMA; HUMANS; MICROTECHNOLOGY; MODELS, THEORETICAL; TISSUE SCAFFOLDS; TRABECULAR MESHWORK;

EID: 84886248155     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24977     Document Type: Article

References (61)

1. Acott TS, Kelley MJ. 2008. Extracellular matrix in the trabecular meshwork. Exp Eye Res 86(4):543-561.
2. Alvarado JA, Betanzos A, Franse-Carman L, Chen J, Gonzalez-Mariscal L. 2004. Endothelia of schlemm's canal and trabecular meshwork: Distinct molecular, functional, and anatomic features. Am J Physiol Cell Physiol 286(3):C621-C634.
3. Alvarado JA, Wood I, Polansky JR. 1982. Human trabecular cells. II. Growth pattern and ultrastructural characteristics. Invest Ophthalmol 23(4):464-478.
4. Bahler CK, Howell KG, Hann CR, Fautsch MP, Johnson DH. 2008. Prostaglandins increase trabecular meshwork outflow facility in cultured human anterior segments. Am J Ophthalmol 145(1):114-119.
5. Bahler CK, Smedley GT, Zhou J, Johnson DH. 2004. Trabecular bypass stents decrease intraocular pressure in cultured human anterior segments. Am J Ophthalmol 138(6):988-994.
6. Brody S, Anilkumar T, Liliensiek S, Last JA, Murphy CJ, Pandit A. 2006. Characterizing nanoscale topography of the aortic heart valve basement membrane for tissue engineering heart valve scaffold design. Tissue Eng 12(2):413-421.
7. Chen J, Runyan S, Robinson M. 2011. Novel ocular antihypertensive compounds in clinical trials. Clin Ophthalmol 5:667-677.
8. Chin VI, Taupin P, Sanga S, Scheel J, Gage FH, Bhatia SN. 2004. Microfabricated platform for studying stem cell fates. Biotechnol Bioeng 88(3):399-415.
9. Diehl KA, Foley JD, Nealey PF, Murphy CJ. 2005. Nanoscale topography modulates corneal epithelial cell migration. J Biomed Mater Res A 75(3):603-611.
10. Elliott NT, Yuan F. 2012. A microfluidic system for investigation of extravascular transport and cellular uptake of drugs in tumors. Biotechnol Bioeng 109(5):1326-1335.
11. Ethier CR, Read AT, Chan DW. 2006. Effects of latrunculin-B on outflow facility and trabecular meshwork structure in human eyes. Invest Ophthalmol 47(5):1991-1998.
12. Fautsch MP, Howell KG, Vrabel AM, Charlesworth MC, Muddiman DC, Johnson DH. 2005. Primary trabecular meshwork cells incubated in human aqueous humor differ from cells incubated in serum supplements. Invest Oophthalmol 46(8):2848-2856.
13. Ferrell N, Desai RR, Fleischman AJ, Roy S, Humes HD, Fissell WH. 2010. A microfluidic bioreactor with integrated transepithelial electrical resistance (TEER) measurement electrodes for evaluation of renal epithelial cells. Biotechnol Bioeng 107(4):707-716.
14. Freddo TF, Patterson MM, Scott DR, Epstein DL. 1984. Influence of mercurial sulfhydryl agents on aqueous outflow pathways in enucleated eyes. Invest Ophthalmol 25(3):278-285.
15. Fugel C, Tamm E, Lutjen-Drecoll E, Stefani F. 1992. Age-related loss of a smooth muscle actin in normal and glaucomatous human trabecular meshwork of different age groups. Glaucoma 1(3):165-173.
16. Gasiorowski JZ, Russell P. 2009. Biological properties of trabecular meshwork cells. Exp Eye Res 88(4):671-675.
17. Grierson I, Marshall J, Robins E. 1983. Human trabecular meshwork in primary culture: A morphological and autoradiographic study. Exp Eye Res 37(4):349-365.
18. Heijl A, Leske MC, Bengtsson B, Hyman L, Hussein M. 2002. Reduction of intraocular pressure and glaucoma progression: Results from the early manifest glaucoma trial. Arch Ophthalmol 120(10):1268-1279.
19. Hennemeyer MWF, Kerstan S, Schurzinger K, Gigler AM, Stark RW. 2008. Cell proliferation assays on plasma activated SU-8. Microelectron Eng 85(5-6):1298-1301.
20. Hung PJ, Lee PJ, Sabounchi P, Lin R, Lee LP. 2005. Continuous perfusion microfluidic cell culture array for high-throughput cell-based assays. Biotechnol Bioeng 89(1):1-8.
21. Jamal M, Bassik N, Cho J-H, Randall CL, Gracias DH. 2010. Directed growth of fibroblasts into three dimensional micropatterned geometries via self-assembling scaffolds. Biomaterials 31(7):1683-1690.
22. Johnson DH. 2005. Trabecular meshwork and uveoscleral outflow models. J Glaucoma 14(4):308-310.
23. Johnson M. 2006. What controls aqueous humour outflow resistance? Exp Eye Res 82(4):545-557.
24. Keller KE, Aga M, Bradley JM, Kelley MJ, Acott TS. 2009. Extracellular matrix turnover and outflow resistance. Exp Eye Res 88(4):676-682.
25. Kim MS, Lee W, Kim YC, Park J-K. 2008. Microvalve-assisted patterning platform for measuring cellular dynamics based on 3D cell culture. Biotechnol Bioeng 101(5):1005-1013.
26. Koga T, Awai M, Tsutsui J, Yue BY, Tanihara H. 2006. Rho-associated protein kinase inhibitor, Y-27632, induces alterations in adhesion, contraction, and motility in cultured human trabecular meshwork cells. Exp Eye Res 82(3):362-370.
27. Kotzar G, Freas M, Abel P, Fleischman A, Roy S, Zorman C, Moran JM, Melzak J. 2002. Evaluation of MEMS materials of construction for implantable medical devices. Biomaterials 23(13):2737-2750.
28. Last JA, Pan T, Ding Y, Reilly CM, Keller K, Acott TS, Fautsch MP, Murphy CJ, Russell P. 2011. Elastic modulus determination of normal and glaucomatous human trabecular meshwork. Invest Ophthalmol Vis Sci 52(5):2147-2152.
29. Lee JT, Chow KL. 2012. SEM sample preparation for cells on 3D scaffolds by freeze-drying and HMDS. Scanning 34(1):12-25.
30. Lee PJ, Hung PJ, Rao VM, Lee LP. 2006. Nanoliter scale microbioreactor array for quantitative cell biology. Biotechnol Bioeng 94(1):5-14.
31. Liliensiek SJ, Campbell S, Nealey PF, Murphy CJ. 2006. The scale of substratum topographic features modulates proliferation of corneal epithelial cells and corneal fibroblasts. J Biomed Mater Res A 79(1):185-192.
32. Llobet A, Gasull X, Gual A. 2003. Understanding trabecular meshwork physiology: A key to the control of intraocular pressure? Physiology 18(5):205-209.
33. Mata A, Fleischman AJ, Roy S, 2006. Fabrication of multi-layer SU-8 microstructure. J Macromech Microeng 30(27):4610-4617.
34. McKee CT, Wood JA, Shah NM, Fischer ME, Reilly CM, Murphy CJ, Russell P. 2011. The effect of biophysical attributes of the ocular trabecular meshwork associated with glaucoma on the cell response to therapeutic agents. Biomaterials 32(9):2417-2423.
35. McLaren JW. 2009. Measurement of aqueous humor flow. Exp Eye Res 88(4):641-647.
36. Overby DR, Stamer WD, Johnson M. 2009. The changing paradigm of outflow resistance generation: Towards synergistic models of the JCT and inner wall endothelium. Exp Eye Res 88(4):656-670.
37. Park J, Li Y, Berthiaume F, Toner M, Yarmush ML, Tilles AW. 2008. Radial flow hepatocyte bioreactor using stacked microfabricated grooved substrates. Biotechnol Bioeng 99(2):455-467.
38. Bogdanov AL, Peredkov SS. 2000. Use of SU-8 photoresist for very high aspect ratio X-ray lithography. Microelect Eng 53:493-496.
39. Perkins TW, Alvarado JA, Polansky JR, Stilwell L, Maglio M, Juster R. 1988. Trabecular meshwork cells grown on filters. Conductivity and cytochalasin effects. Invest Ophthalmol Vis Sci 29(12):1836-1846.
40. Peterson JA, Tian B, Geiger B, Kaufman PL. 2000. Effect of latrunculin-B on outflow facility in monkeys. Exp Eye Res 70(3):307-313.
41. Polansky JR, Weinreb RN, Baxter JD, Alvarado J. 1979. Human trabecular cells. I. Establishment in tissue culture and growth characteristics. Invest Ophthalmol Vis Sci 18(10):1043-1049.
42. Polansky JR, Wood IS, Maglio MT, Alvarado JA. 1984. Trabecular meshwork cell culture in glaucoma research: Evaluation of biological activity and structural properties of human trabecular cells in vitro. Ophthalmology 91(6):580-595.
43. Resch ZT, Hann CR, Cook KA, Fautsch MP. 2010. Aqueous humor rapidly stimulates myocilin secretion from human trabecular meshwork cells. Exp Eye Res 91(6):901-908.
44. Russell P, Gasiorowski JZ, Nealy PF, Murphy CJ. 2008. Response of human trabecular meshwork cells to topographic cues on the nanoscale level. Invest Ophthalmol Vis Sci 49(2):629-635.
45. Sabanay I, Tian B, Gabelt BAT, Geiger B, Kaufman PL. 2006. Latrunculin B effects on trabecular meshwork and corneal endothelial morphology in monkeys. Exp Eye Res 82(2):236-246.
46. Schlunck G, Han H, Wecker T, Kampik D, Meyer-ter-Vehn T, Grehn F. 2008. Substrate rigidity modulates cell matrix interactions and protein expression in human trabecular meshwork cells. Invest Ophthalmol Vis Sci 49(1):262-269.
47. Shen X, Koga T, Park BC, SundarRaj N, Yue BY. 2008. Rho GTPase and cAMP/protein kinase A signaling mediates myocilin-induced alterations in cultured human trabecular meshwork cells. J Biol Chem 283(1):603-612.
48. Spector I, Shochet NR, Kashman Y, Groweiss A. 1983. Latrunculins: Novel marine toxins that disrupt microfilament organization in cultured cells. Science 219(4584):493-495.
49. Spencer WH, Alvarado J, Hayes TL. 1968. Scanning electron microscopy of human ocular tissues: Trabecular meshwork. Invest Ophthalmol 7(6):651-662.
50. Sugiura S, Edahiro J-i, Kikuchi K, Sumaru K, Kanamori T. 2008. Pressure-driven perfusion culture microchamber array for a parallel drug cytotoxicity assay. Biotechnol Bioeng 100(6):1156-1165.
51. Tamm ER. 2009. The trabecular meshwork outflow pathways: Structural and functional aspects. Exp Eye Res 88(4):648-655.
52. Tan JC, Gonzalez JM Jr, Hamm-Alvarez S, Song J. 2012. In situ autofluorescence visualization of human trabecular meshwork structure. Invest Ophthalmol Vis Sci 53(4):2080-2088.
53. Tektas OY, Lutjen-Drecoll E. 2009. Structural changes of the trabecular meshwork in different kinds of glaucoma. Exp Eye Res 88(4):769-775.
54. Thomasy SM, Wood JA, Kass PH, Murphy CJ, Russell P. 2012. Substratum stiffness and latrunculin B regulate matrix gene and protein expression in human trabecular meshwork cells. Invest Ophthalmol Vis Sci 53(2):952-958.
55. Tian B, Geiger B, Epstein DL, Kaufman PL. 2000. Cytoskeletal involvement in the regulation of aqueous humor outflow. Invest Ophthalmol Vis Sci 41(3):619-623.
56. Titmarsh D, Cooper-White J. 2009. Microbioreactor array for full-factorial analysis of provision of multiple soluble factors in cellular microenvironments. Biotechnol Bioeng 104(6):1240-1244.
57. Vaajanen A, Vapaatalo H, Oksala O. 2007. A modified in vitro method for aqueous humor outflow studies in enucleated porcine eyes. J Ocul Pharmacol Ther 23(2):124-131.
58. Voskerician G, Shive MS, Shawgo RS, von Recum H, Anderson JM, Cima MJ, Langer R. 2003. Biocompatibility and biofouling of MEMS drug delivery devices. Biomaterials 24(11):1959-1967.
59. Welge-Lussen U, May CA, Eichhorn M, Bloemendal H, Lutjen-Drecoll E. 1999. AlphaB-crystallin in the trabecular meshwork is inducible by transforming growth factor-beta. Invest Ophthalmol Vis Sci 40(10):2235-2241.
60. Wentz-Hunter K, Kubota R, Shen X, Yue BY. 2004. Extracellular myocilin affects activity of human trabecular meshwork cells. J Cell Physiol 200(1):45-52.
61. Wood JA, McKee CT, Thomasy SM, Fischer ME, Shah NM, Murphy CJ, Russell P. 2011. Substratum compliance regulates human trabecular meshwork cell behaviors and response to latrunculin B. Invest Ophthalmol Vis Sci 52(13):9298-9303.