An Efficient Strategy for Small-Scale Screening and Production of Archaeal Membrane Transport Proteins in Escherichia coli

PLoS ONE

Volumn 8, Issue 10, 2013, Pages

  Ma, Pikyee ^a   Varela, Filipa ^a   Magoch, Malgorzata ^a   Silva, Ana Rita ^a   Rosário, Ana Lúcia ^a   Brito, José ^a   Oliveira, Tânia Filipa ^a   Nogly, Przemyslaw ^a   Pessanha, Miguel ^a   Stelter, Meike ^a,d   Kletzin, Arnulf ^b   Henderson, Peter J F ^c   Archer, Margarida ^a  

^a INSTITUTO DE TECNOLOGIA QUÍMICA E BIOLÓGICA   (Portugal)

^b DARMSTADT UNIVERSITY OF TECHNOLOGY   (Germany)

^c UNIVERSITY OF LEEDS   (United Kingdom)

^d CEA GRENOBLE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

CARRIER PROTEIN; COTRANSPORTER; NEUROTRANSMITTER; NEUROTRANSMITTER SODIUM SYMPORTER; NUCLEIC ACID BASE; NUCLEOBASE CATION SYMPORTER 1; NUCLEOBASE CATION SYMPORTER 2; SUGAR; SUGAR PORTER; UNCLASSIFIED DRUG; VITAMIN;

AMINO ACID SEQUENCE; ARCHAEON; ARTICLE; BACTERIAL STRAIN; CONTROLLED STUDY; CRYSTALLIZATION; ESCHERICHIA COLI; EXPRESSION VECTOR; GEL PERMEATION CHROMATOGRAPHY; GENE; GENE AMPLIFICATION; GENE EXPRESSION; HETEROLOGOUS EXPRESSION; HIGH THROUGHPUT SCREENING; METABOLITE; NONHUMAN; PREDICTION; PROTEIN PURIFICATION; PROTEIN STABILITY; PROTEIN TRANSPORT; SEQUENCE ALIGNMENT; SMALL SCALE SCREENING; TRANSPORTER GENE;

ARCHAEA; ARCHAEAL PROTEINS; CHROMATOGRAPHY, GEL; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION; MEMBRANE TRANSPORT PROTEINS; RECOMBINANT PROTEINS;

EID: 84885028668     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0076913     Document Type: Article

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