Topology-informed strategies for the overexpression and purification of membrane proteins

Molecular Membrane Biology

Volumn 24, Issue 5-6, 2007, Pages 407-418

  Rahman, Moazur ^a,b   Ismat, Fouzia ^a   McPherson, Michael J J ^a   Baldwin, Stephen A ^a  

^a UNIVERSITY OF LEEDS   (United Kingdom)

^b NATIONAL INSTITUTE FOR BIOTECHNOLOGY AND GENETIC ENGINEERING NIBGE   (Pakistan)

Author keywords

Membrane protein; Overexpression; Purification; Structure; Topology

Indexed keywords

MEMBRANE PROTEIN;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; CRYSTAL STRUCTURE; CYTOPLASM; GENE OVEREXPRESSION; GENOME ANALYSIS; HIGH THROUGHPUT SCREENING; LARGE SCALE PRODUCTION; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; STRUCTURAL GENOMICS; STRUCTURE ANALYSIS;

BLOTTING, WESTERN; ESCHERICHIA COLI PROTEINS; GENETIC VECTORS; MEMBRANE PROTEINS; PERIPLASM; RECOMBINANT FUSION PROTEINS; SYMPORTERS;

BACTERIA (MICROORGANISMS);

EID: 34548072799     PISSN: 09687688     EISSN: 14645203     Source Type: Journal    
DOI: 10.1080/09687860701243998     Document Type: Article

References (26)

1. Krogh A, Larsson B, von Heijne G, Sonnhammer EL. 2001. Predicting transmembrane protein topology with a hidden markov model: application to complete genomes. J Mol Biol 305:567-580.
2. Hopkins AL, Groom CR. 2002. The druggable genome. Nat Rev Drug Discov 1:727-730.
3. Ren Q, Paulsen IT. 2005. Comparative analyses of fundamental differences in membrane transport capabilities in prokaryotes and eukaryotes. PLoS Comput Biol 1:e27.
4. Wang DN, Safferling M, Lemieux MJ, Griffith H, Chen Y, Li XD. 2003. Practical aspects of overexpressing bacterial secondary membrane transporters for structural studies. Biochim Biophys Acta 1610:23-36.
5. Eshaghi S, Hedren M, Nasser MI, Hammarberg T, Thornell A, Nordlund P. 2005. An efficient strategy for high-throughput expression screening of recombinant integral membrane proteins. Protein Sci 14:676-683.
6. Drew DE, von Heijne G, Nordlund P, de Gier JW. 2001. Green fluorescent protein as an indicator to monitor membrane protein overexpression in Escherichia coli. FEBS Lett 507:220-224.
7. Kawate T, Gouaux E. 2006. Fluorescence-detection size-exclusion chromatography for precrystallization screening of integral membrane proteins. Structure 14:673-681.
8. Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G. 2005. Global topology analysis of the Escherichia coli inner membrane proteome. Science 308:1321-1323.
9. Granseth E, Daley DO, Rapp M, Melen K, von Heijne G. 2005. Experimentally constrained topology models for 51,208 bacterial inner membrane proteins. J Mol Biol 352:489-494.
10. Don RH, Cox PT, Wainwright BJ, Baker K, Mattick JS. 1991. "Touchdown" PCR to circumvent spurious priming during gene amplification. Nucleic Acids Res 19:4008-4008.
11. Xie H, Patching SG, Gallagher MP, Litherland GJ, Brough AR, Venter H, Yao SY, Ng AM, Young JD, Herbert RB, Henderson PJ, Baldwin SA. 2004. Purification and properties of the Escherichia coli nucleoside transporter NupG, a paradigm for a major facilitator transporter sub-family. Mol Membr Biol 21:323-336.
12. Stark MJR. 1987. Multicopy expression vectors carrying the lac repressor gene for regulated high-level expression of genes in Escherichia coli. Gene 51:255-267.
13. Hadden DA. 2002. Molecular mechanisms of nucleoside and nucleobase transporters [thesis]. Leeds (Yorkshire, UK): University of Leeds.
14. Skerra A, Schmidt TG. 2000. Use of the Strep-Tag and streptavidin for detection and purification of recombinant proteins. Methods Enzymol 326:271-304.
15. Ward A, Sanderson NM, O'Reilly J, Rutherford NG, Poolman B, Henderson PJF. 2000. The amplified expression, identification, purification, assay and properties of histidine-tagged bacterial membrane transport proteins. In: Baldwin SA, editor. Membrane transport - a practical approach. Oxford: Oxford University Press. p. 141-166.
16. Henderson PJF, Macpherson AJ. 1986. Assay, genetics, proteins and reconstitution of proton-linked galactose, arabinose and xylose transport systems of Escherichia coli. Methods Enzymol 125:387-429.
17. Walhout AJ, Temple GF, Brasch MA, Hartley JL, Lorson MA, van den Heuvel S, Vidal M. 2000. GATEWAY recombinational cloning: application to the cloning of large numbers of open reading frames or ORFeomes. Methods Enzymol 328:575-592.
18. Nilsson J, Persson B, von Heijne G. 2005. Comparative analysis of amino acid distributions in integral membrane proteins from 107 genomes. Proteins 60:606-616.
19. Salaun C, Rodrigues P, Heard JM. 2001. Transmembrane topology of PiT-2, a phosphate transporter-retrovirus receptor. J Virol 75:5584-5592.
20. Courville P, Chaloupka R, Veyrier F, Cellier MF. 2004. Determination of transmembrane topology of the Escherichia coli natural resistance-associated macrophage protein (Nramp) ortholog. J Biol Chem 279:3318-3326.
21. +/Proline transporter of Escherichia coli. J Biol Chem 273:26400-26407.
22. Gendreau S, Voswinkel S, Torres-Salazar D, Lang N, Heidtmann H, Detro-Dassen S, Schmalzing G, Hidalgo P, Fahlke C. 2004. A trimeric quaternary structure is conserved in bacterial and human glutamate transporters. J Biol Chem 279:39505-39512.
23. Yernool D, Boudker O, Jin Y, Gouaux E. 2004. Structure of a glutamate transporter homologue from Pyrococcus horikoshii . Nature 431:811-818.
24. Goormaghtigh E, Cabiaux V, Ruysschaert JM. 1994. Determination of soluble and membrane protein structure by Fourier transform infrared spectroscopy. III. Secondary structures. Subcell Biochem 23:405-450.
25. Arrondo JL, Goni FM. 1999. Structure and dynamics of membrane proteins as studied by infrared spectroscopy. Prog Biophys Mol Biol 72:367-405.
26. Drew D, Slotboom DJ, Friso G, Reda T, Genevaux P, Rapp M, Meindl-Beinker NM, Lambert W, Lerch M, Daley DO, Van Wijk KJ, Hirst J, Kunji E, de Gier JW. 2005. A scalable, GFP-based pipeline for membrane protein overexpression screening and purification. Protein Sci 14:2011-2017.