An efficient strategy for high throughput screening of recombinant integral membrane protein expression and stability

Protein Expression and Purification

Volumn 78, Issue 1, 2011, Pages 6-13

  Fan, Junping ^a,b   Heng, Jie ^a   Dai, Shuyan ^a   Shaw, Neil ^a   Zhou, Bei ^a   Huang, Bo ^a   He, Zheng ^a   Wang, Ya ^a   Jiang, Taijiao ^a   Li, Xuemei ^a   Liu, Zhijie ^a   Wang, Xianping ^a   Zhang, Xuejun C ^a  

^a INSTITUTE OF BIOPHYSICS   (China)

^b BEIJING NORMAL UNIVERSITY   (China)

Author keywords

Green fluorescence protein; High throughput; Ligation independent cloning; Protein expression; Thermofluor; Transmembrane proteins

Indexed keywords

ESCHERICHIA COLI PROTEIN; FLUORESCENT DYE; HYBRID PROTEIN; MEMBRANE PROTEIN;

ARTICLE; CHEMISTRY; ESCHERICHIA COLI; GENE VECTOR; GENETICS; HIGH THROUGHPUT SCREENING; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR CLONING; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN STABILITY;

CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FLUORESCENT DYES; GENETIC VECTORS; HIGH-THROUGHPUT SCREENING ASSAYS; MEMBRANE PROTEINS; PROTEIN STABILITY; RECOMBINANT FUSION PROTEINS;

ESCHERICHIA COLI; PROKARYOTA;

EID: 79955612777     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2011.02.010     Document Type: Article

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