Quantification of hepcidin-25 in human serum by isotope dilution micro-HPLC-tandem mass spectrometry

Clinical Chemistry

Volumn 54, Issue 9, 2008, Pages 1584-1586

  Kobold, Uwe ^a   Dülffer, Thomas ^a   Dangl, Markus ^a   Escherich, Achim ^b   Kubbies, Manfred ^a   Röddiger, Ralf ^a   Wright, Jo Anne ^a  

^a ROCHE DIAGNOSTICS GMBH   (Germany)

^b Roche Diagnostics AG   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

FERROPORTIN; HEPCIDIN; HEPCIDIN 25; UNCLASSIFIED DRUG;

BLOOD SAMPLING; DIAGNOSTIC ACCURACY; ELECTROSPRAY; FEMALE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; IRON DEFICIENCY; ISOTOPE DILUTION ASSAY; LETTER; MALE; PROTEIN ANALYSIS; SERUM; TANDEM MASS SPECTROMETRY;

ANTIMICROBIAL CATIONIC PEPTIDES; CARBON ISOTOPES; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; FASTING; FEMALE; HEALTH; HUMANS; INDICATOR DILUTION TECHNIQUES; MALE; NITROGEN ISOTOPES; TANDEM MASS SPECTROMETRY;

EID: 51349114252     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1373/clinchem.2008.107029     Document Type: Letter

References (5)

1. Ganz T. Hepcidin - a peptide hormone at the interface of innate immunity and iron metabolism. Curr Top Microbiol Immunol 2006;306:183-98.
2. Kemna EH, Tjalsma H, Willems HL, Swinkels DW. Hepcidin: from discovery to differential diagnosis. Haematologica 2008;93:90-7.
3. Toll H, Wintringer R, Schweiger-Hufnage U, Huber CG. Comparing monolithic and microparticular capillary columns for the separation and analysis of peptide mixtures by liquid chromatography-mass spectrometry. J Sep Sci (Wash DC) 2005;28:1666-74.
4. Laut, X, Babon JJ, Stannard JA, Singh S, Nizet V, Carlberg JM, et al. Bass hepcidin synthesis, solution structure, antimicrobial activities and synergism, and in vivo hepatic response to bacterial infections. J Biol Chem 2005;280:9272-82.
5. Kemna EH, Tjalsma H, Podust VN, Swinkels DW. Mass spectrometry-based hepcidin measurements in serum and urine: analytical aspects and clinical implications. Clin Chem 2007;53:620-8.