A novel multimodal chromatography based single step purification process for efficient manufacturing of an E. coli based biotherapeutic protein product

Journal of Chromatography A

Volumn 1314, Issue , 2013, Pages 188-198

  Bhambure, Rahul ^a   Gupta, Darpan ^a   Rathore, Anurag S ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY DELHI   (India)

Author keywords

Capto MMC; Granulocyte colony stimulating factor (GCSF); HEA hypercel; High throughput process development (HTPD); Hydrophobic charge induction chromatography (HCIC); Multimodal chromatography; PPA hypercel

Indexed keywords

CAPTO MMC; GRANULOCYTE-COLONY STIMULATING FACTOR; HEA HYPERCEL; HIGHTHROUGHPUT PROCESS DEVELOPMENT (HTPD); HYDROPHOBIC CHARGE INDUCTION CHROMATOGRAPHIES; MULTIMODAL CHROMATOGRAPHIES; PPA HYPERCEL;

AMINO ACIDS; BIOACTIVITY; CHROMATOGRAPHIC ANALYSIS; ESCHERICHIA COLI; HYDROPHOBICITY; PURIFICATION; RECOMBINANT PROTEINS; REMOVAL; SALT REMOVAL;

HYDROPHOBIC CHROMATOGRAPHY;

GRANULOCYTE COLONY STIMULATING FACTOR; METHIONINE; RECOMBINANT PROTEIN; SODIUM CHLORIDE;

ANIMAL CELL; ARTICLE; BIOLOGICAL ACTIVITY; CHROMATOGRAPHY; CIRCULAR DICHROISM; CONTROLLED STUDY; DRUG IMPURITY; DRUG MANUFACTURE; DRUG PURIFICATION; ELUTION; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HOST CELL; IMMUNOGENIC RESPONSE; LIGAND BINDING; MOUSE; MULTIMODAL CHROMATOGRAPHY; NONHUMAN; OXIDATION; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN SECONDARY STRUCTURE; PROTEIN STRUCTURE; ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY;

CAPTO MMC; GRANULOCYTE COLONY STIMULATING FACTOR (GCSF); HEA HYPERCEL; HIGH THROUGHPUT PROCESS DEVELOPMENT (HTPD); HYDROPHOBIC CHARGE INDUCTION CHROMATOGRAPHY (HCIC); MULTIMODAL CHROMATOGRAPHY; PPA HYPERCEL;

BIOLOGICAL PRODUCTS; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CIRCULAR DICHROISM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; GRANULOCYTE COLONY-STIMULATING FACTOR;

BACTERIA (MICROORGANISMS);

EID: 84884979732     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2013.09.026     Document Type: Article

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