MEP HyperCel chromatography II: Binding, washing and elution

Protein Expression and Purification

Volumn 71, Issue 2, 2010, Pages 168-173

  Arakawa, Tsutomu ^a   Futatsumori Sugai, Mutsumi ^b   Tsumoto, Kouhei ^b   Kita, Yoshiko ^c   Sato, Haruna ^d   Ejima, Daisuke ^d  

^a ALLIANCE PROTEIN LABORATORIES   (United States)

^b UNIVERSITY OF TOKYO   (Japan)

^c KEIO UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^d AJINOMOTO CO INC   (Japan)

Author keywords

Antibodies; Arginine; Elution; Hydrophobic interaction; MEP Hypercel

Indexed keywords

2 PROPANOL; ANTIBODY; ARGININE; BOVINE SERUM ALBUMIN; IMMUNOGLOBULIN; MONOCLONAL ANTIBODY; PROTEIN; RECOMBINANT PROTEIN; STAPHYLOCOCCUS PROTEIN A; UREA; WATER;

ANIMAL; ARTICLE; CATTLE; CHEMISTRY; CHROMATOGRAPHY; HYDROPHOBICITY; METABOLISM; METHODOLOGY; SILKWORM; SOLUTION AND SOLUBILITY;

2-PROPANOL; ANIMALS; ANTIBODIES; ANTIBODIES, MONOCLONAL; ARGININE; BOMBYX; CATTLE; CHROMATOGRAPHY; HYDROPHOBICITY; IMMUNOGLOBULINS; PROTEINS; RECOMBINANT PROTEINS; SERUM ALBUMIN, BOVINE; SOLUTIONS; STAPHYLOCOCCAL PROTEIN A; UREA; WATER;

BOMBYX MORI; BOVINAE; MAMMALIA;

EID: 77949491751     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2009.11.004     Document Type: Article

References (11)

1. Schwart W., Judd D., Wysocki M., Guerrier L., Birck-Wilson E., and Boschetti E. Comparison of hydrophobic charge induction chromatography with affinity chromatography on protein A for harvest and purification of antibodies. J. Chromatogr. A 908 (2001) 251-263
2. Ghose S., Hubbard B., and Cramer S.M. Protein interactions in hydrophobic charge induction chromatography (HCIC). Biotechnol. Prog. 21 (2005) 498-508
3. Ghose S., Hubbard B., and Cramer S.M. Evaluation and comparison of alternatives to protein A chromatography mimetic and hydrophobic charge induction chromatography stationary phases. J. Chromatogr. A 1122 (2006) 144-1452
4. Gagnon P. IgG aggregate removal by charge-hydrophobic mixed mode chromatography. Curr. Pharm. Biotechnol. 10 (2009) 434-439
5. Arakawa T., Kita Y., Sato H., and Ejima D. MEP chromatography of antibody and Fc-fusion protein using aqueous arginine solution. Protein Expr. Purif. 54 (2009) 110-116
6. S. Barron, C. Major, S. Cross, J. Madan, F. Mann, Improving purity on protein A affinity chromatography media through use of an arginine intermediate wash step, Millipore Technical Brief, TB1024EN00 (2005).
7. Shukla A.A., and Hinkley P. Host cell protein clearance during protein A chromatography: development of an improved column wash step. Biotechnol. Prog. 24 (2008) 1115-1121
8. Fahrner R., Knudsen H., Basey C., Galan W., Feuerhelm D., Vanderlaan M., and Blank G. Industrial purification of pharmaceutical antibodies: development, operation and validation of chromatography processes. Biotechnol. Gen. Eng. Rev. 18 (2001) 301-327
9. Yumioka R., Tsumoto K., Arakawa T., and Ejima D. Screening of effective rinse solvent for protein-A chromatography. Protein Expr. Purif. 70 (2010) 218-223
10. Durkee K.H., Roh B.H., and Doellgast G.J. Immunoaffinity chromatographic purification of Russell's viper factor X activator using elution in high concentrations of magnesium chloride. Protein Expr. Purif. 4 (1993) 405-411
11. Kummer A., and Li-Chan E.C. Application of an ELISA-elution assay as a screening for dissociation of yolk antibody-antigen complexes. I. Immunol. Methods 211 (1998) 125-137