Analysis for residual host cell proteins and DNA in process streams of a recombinant protein product expressed in Escherichia coli cells

Journal of Pharmaceutical and Biomedical Analysis

Volumn 32, Issue 6, 2003, Pages 1199-1211

  Rathore, Anurag Singh ^a   Sobacke, S E ^b   Kocot, T J ^b   Morgan, D R ^b   Dufield, R L ^b   Mozier, N M ^b  

^a AMGEN INC   (United States)

^b PFIZER INC   (United States)

Author keywords

Chromatography; DNA; E. coli inclusion bodies; HCP; Host cell impurities; Impurity clearance; Ion exchange resins; Protein purification

Indexed keywords

CELL PROTEIN; RECOMBINANT PROTEIN; RESIN;

ARTICLE; BACTERIAL CELL; BIOPROCESS; BIOTECHNOLOGY; CELL INCLUSION; CENTRIFUGATION; CHROMATOGRAPHY; DNA DETERMINATION; ELUTION; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; EXPRESSION VECTOR; HOST CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN ISOLATION; PROTEIN PROCESSING; PROTEIN PURIFICATION; QUANTITATIVE ANALYSIS; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

EID: 0042202405     PISSN: 07317085     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0731-7085(03)00157-2     Document Type: Article

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