Study of the conditions for multi-modal chromatographic capture of Fab′ from dual-salt precipitated E. coli homogenate

Journal of Chemical Technology and Biotechnology

Volumn 88, Issue 3, 2013, Pages 372-377

  Balasundaram, Bangaru ^a   Chhatre, Sunil ^a   Arora, Dhruv ^a   Titchener Hooker, Nigel J ^a   Bracewell, Daniel G ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

Capto MMC; Dual salt precipitation; Fab ; Multi modal chromatography

Indexed keywords

AMMONIUM SULPHATE; ANTIBODY FRAGMENT; BINDING CAPACITIES; CAPTO MMC; CATION EXCHANGE RESINS; CO-ADSORPTION; E. COLI; HIGH THROUGHPUT SCREENING; HYDROPHOBIC BINDING; IMMUNOTHERAPEUTICS; MICRO-SCALES; MULTI-MODAL; PRECIPITATION TECHNIQUES; PROTEIN A; PROTEIN A CHROMATOGRAPHY; SALT PRECIPITATION;

AMMONIUM COMPOUNDS; CHROMATOGRAPHY; ESCHERICHIA COLI; IMPURITIES; ION EXCHANGE; IONIC STRENGTH; LASERS; LEACHING; PROTEINS; REMOVAL; SALT REMOVAL;

PRECIPITATION (CHEMICAL);

AMMONIUM SULFATE; IMMUNOGLOBULIN F(AB) FRAGMENT; PROTEIN; RESIN;

ARTICLE; BINDING AFFINITY; ESCHERICHIA COLI; HIGH THROUGHPUT SCREENING; HOMOGENATE; HYDROPHOBICITY; ION EXCHANGE CHROMATOGRAPHY; IONIC STRENGTH; NONHUMAN; PIPETTE; PRECIPITATION; RAT;

EID: 84874017960     PISSN: 02682575     EISSN: 10974660     Source Type: Journal    
DOI: 10.1002/jctb.3839     Document Type: Article

References (20)

1. Gottschalk U, Bioseparation in antibody manufacturing: the good, the bad and the ugly. Biotechnol Prog 24: 496-503 (2008).
2. Low D, O'Leary R Pujar NS, Future of antibody purification. J Chromatogr B 848: 48-63 (2008).
3. Thömmes J Etzel M, Alternatives to chromatographic separations. Biotechnol Prog 23: 42-45 (2007).
4. Przybycien TM, Pujar NS Steele LM, Alternative bioseparation operations: life beyond packed-bed chromatography. Curr Opin Biotechnol 15: 469-478 (2004).
5. Balasundaram B, Sachdeva S Bracewell D, Dual salt precipitation for the recovery of a recombinant protein from E. coli. Biotechnol Prog 27: 1306-1314 (2011).
6. McDonald P, Victa C, Carter-Franklin JN Fahrner R, Selective antibody precipitation using polyelectrolytes: a novel approach to the purification of monoclonal antibodies. Biotechnol Bioeng 102: 1141-1151 (2009).
7. Persson J Lester P, Purification of antibody and antibody-fragment from E. coli homogenate using 6, 9-diamino-2-ethoxyacridine lactate as precipitation agent. Biotechnol Bioeng 87: 424-434 (2004).
8. Peram T, McDonald P, Carter-Franklin J Fahrner R, Monoclonal antibody purification using cationic polyelectrolytes: an alternative to column chromatography. Biotechnol Prog 26: 1332-1331 (2010).
9. Chen J, Tetrault J, Zhang Y, Wasserman A, Conley G, DiLeo M, et al, The distinctive separation attributes of mixed-mode resins and their application in monoclonal antibody downstream purification process. J Chromatogr A. 1217: 216-224 (2010).
10. Guerrier L, Flayeux I Boschetti E, A dual-mode approach to the selective separation of antibodies and their fragments. J Chromatogr B 755: 37-46 (2001).
11. Kaleas KA, Schmelzer CH Pizarro SA, Industrial case study: evaluation of a mixed-mode resin for selective capture of a human growth factor recombinantly expressed in E. coli. J Chromatogr A 1217: 235-242 (2010).
12. Tustian AD, Salte H, Willoughby N, Hassan I, Rose M, Baganz F, et al, Adapted ultra scale-down approach for predicting the centrifugal separation behavior of high cell density cultures. Biotechnol Prog 23: 1404-1410 (2007).
13. Ljunglöf A, La̧cki KM, Mueller J, Harinarayan C, van Reis R, Fahrner R, et al, Ion exchange chromatography of antibody fragments. Biotechnol Bioeng 96: 515-524 (2007).
14. Wenger MD, DePhillips P, Price CE Bracewell DG, An automated microscale chromatographic purification of virus-like particles as a strategy for process development. Biotechnol Appl Biochem 47: 131-139 (2007).
15. Chhatre S, Bracewell DG Titchener-Hooker NJ, A microscale approach for predicting the performance of chromatography columns used to recover therapeutic polyclonal antibodies. J Chromatogr A 1216: 7806-7815 (2009).
16. Chhatre S, Francis R, Bracewell DG Titchener-Hooker NJ, An automated packed Protein G micro-pipette tip assay for rapid quantification of polyclonal antibodies in ovine serum. J Chromatogr B 878: 3067-3075 (2010).
17. Bradford MM, Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254 (1976).
18. Harinarayan C, Mueller J, Ljunglöf A, Fahrner R, Van Alstine J van Reis R, An exclusion mechanism in ion exchange chromatography. Biotechnol Bioeng 95: 775-787 (2006).
19. Stein A Kiesewetter A, Cation exchange chromatography in antibody purification: pH screening for optimised binding and HCP removal. J Chromatogr B 848: 151-158 (2007).
20. Tugcu N, Roush DJ Göklen KE, Maximizing productivity of chromatography steps for purification of monoclonal antibodies. Biotechnol Bioeng 99: 599-613 (2008).