Evaluation of the GADD45α-GFP GreenScreen HC assay for rapid and reliable in vitro early genotoxicity screening

Journal of Applied Toxicology

Volumn 33, Issue 11, 2013, Pages 1303-1315

  Luzy, Anne Pascale ^a   Orsini, Nicolas ^a   Linget, Jean Michel ^a   Bouvier, Guy ^a  

^a Galderma R and D   (France)

Author keywords

Ames test; Genotoxicity in vitro screening; Green Screen HC assay; In vitro micronucleus; SOS ChromoTest

Indexed keywords

CARBARIL; CARBENDAZIM; CLINDAMYCIN; DEXAMETHASONE; GREEN FLUORESCENT PROTEIN; GROWTH ARREST AND DNA DAMAGE INDUCIBLE PROTEIN 45; GROWTH ARREST AND DNA DAMAGE INDUCIBLE PROTEIN 45 ALPHA; HYDROQUINONE; LYMECYCLINE; MEQUINOL; METRONIDAZOLE; NEOMYCIN; RESORCINOL; ROSIGLITAZONE; TETRACYCLINE; UNCLASSIFIED DRUG; CELL CYCLE PROTEIN; GADD45A PROTEIN, HUMAN; MUTAGENIC AGENT; NUCLEAR PROTEIN;

AMES TEST; ARTICLE; CONTROLLED STUDY; GENOTOXICITY; IN VITRO STUDY; MICRONUCLEUS TEST; MINI AMES TEST; PRIORITY JOURNAL; SOS CHROMOTEST; TOXICITY TESTING; ANIMAL; BIOSYNTHESIS; CELL LINE; GENETICS; GREEN CHEMISTRY; HUMAN; METABOLISM; MUTAGEN TESTING; PROCEDURES; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; SPRAGUE DAWLEY RAT;

BACTERIA (MICROORGANISMS);

ANIMALS; CELL CYCLE PROTEINS; CELL LINE; GREEN CHEMISTRY TECHNOLOGY; GREEN FLUORESCENT PROTEINS; HUMANS; MUTAGENICITY TESTS; MUTAGENS; NUCLEAR PROTEINS; RATS, SPRAGUE-DAWLEY; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY;

EID: 84884702584     PISSN: 0260437X     EISSN: 10991263     Source Type: Journal    
DOI: 10.1002/jat.2793     Document Type: Article

References (47)

1. Alpers DH, Grimme N. 1978. Effect of broad-spectrum antibiotics on in vitro synthesis of DNA in the rabbit colon. J. Infect. Dis. 137: 756-763.
2. Avandia. FDA Prescription information 2007 [WWW document]. URL http://www.fda.gov/downloads/Drugs/DrugSafety/PostmarketDrugSafetyInformationforPatientsandProviders/UCM143413nd.pdf [accessed on 16 April 2012].
3. Banduhn N, Obe G. 1985. Mutagenicity of methyl 2-benzimidazolecarbamate, diethylstilbestrol and estradiol: structural chromosomal aberrations, sister-chromatid exchanges, C-mitoses, polyploidies and micronuclei. Mutat. Res. 156: 199-218.
4. Bedir A. Aliyazicioglu Y, Kahraman H, Yurdaku Z, Uysal M, Suvaci DE, Okuyucu A, Hökelek M, Alvur M. 2006. Genotoxicity in rats treated with the antidiabetic agent, rosiglitazone. Environ. Mol. Mutagen. 47: 718-724.
5. Bendesky A, Menéndez D, Ostrosky-Wegman P. 2002. Is metronidazole carcinogenic? Mutat. Res. 511: 133-144.
6. Bentley KS, Kirkland D, Murphy M, Marshall R. 2000. Evaluation of thresholds for benomyl- and carbendazim-induced aneuploidy in cultured human lymphocytes using fluorescence in situ hybridization. Mutat. Res. 464: 41-51.
7. Bhattacharjee SB, Bijanbihari P. 1982. Tetracycline-induced mutation in cultured Chinese hamster cells. Mutat. Res. 101: 329-338.
8. Billinton N, Hastwell PW, Beerens D, Birrell L, Ellis P, Maskell S, Webster TW, Windebank S, Woestenborghs F, Lynch AM, Scott AD, Tweats DJ, van Gompel J, Rees RW, Walmsley RM. 2008. Interlaboratory assessment of the GreenScreen HC GADD45a-GFP genotoxicity screening assay: An enabling study for independent validation as an alternative method. Mutat. Res. 653: 23-33.
9. Billinton N, Bruce S, Hansen, JR, Hastwell PW, Jagger C, McComb C, Klu, ML, Pant K, Rabinowitz A, Rees R, Tate M, Vinggaard AM, Walmsley RM. 2010. A pre-validation transferability study of the GreenScreen HC GADD45a-GFP assay with a metabolic activation system (S9). Mutat. Res. 700: 44-50.
10. Birrell L, Cahill P, Hughes C, Tate M, Walmsley RM. 2010. GADD45a-GFP GreenScreen HC assay results for the ECVAM recommended lists of genotoxic and non-genotoxic chemicals for assessment of new genotoxicity tests. Mutat. Res. 695: 87-95.
11. Bruemmer D, Yin F, Liu J, Berger JP, Sakai T, Blaschke F, Fleck E, Van Herle AJ, Forman BM, Law RE. 2003. Regulation of the growth arrest and DNA damage-inducible gene 45 (GADD45) by peroxisome proliferator-activated receptor gamma in vascular smooth muscle cells. Circ. Res. 93: 38-47.
12. Darroudi F, Natarajan AT. 1983. Cytogenetic analysis of human peripheral blood lymphocytes (in vitro) treated with resorcinol. Mutat. Res. 124: 179-189.
13. Dayan J, Crajer MC, Deguingand S. 1982. Mutagenic activity of 4 active-principle forms of pharmaceutical drugs. Comparative study in the Salmonella thyphimurium microsome test, and the HGPRT and Na+/K+ATPase systems in cultured mammalian cells. Mutat. Res. 102: 1-12.
14. DeCaprio AP. 1999. The toxicology of hydroquinone - relevance to occupational and environmental exposure. Crit. Rev. Toxicol. 29: 283-330.
15. Delescluse C, Ledirac N, Rouya L, Piechocki MP, Hines RN, Gidrol X, Rahmani R. 2001. Induction of cytochrome P450 1A1 gene expression, oxidative stress, and genotoxicity by carbaryl and thiabendazole in transfected human HepG2 and lymphoblastoid cells. Biochem. Pharmacol. 61: 399-407.
16. Greenwood SK, Hill RB, Sun JT, Armstrong MJ, Johnson TE, Gara JP, Galloway SM. 2004. Population doubling: a simple and more accurate estimation of cell growth suppression in the in vitro assay for chromosomal aberrations that reduces irrelevant positive results. Environ. Mol. Mutagen. 43: 36-44.
17. Hastwell PW, Chai L-L, Roberts KJ, Webster TW, Harvey JS, Rees RW, Walmsley RM. 2006. High-specificity and high-sensitivity genotoxicity assessment in a human cell line: validation of the GreenScreen HC genotoxicity assay. Mutat. Res. 607: 160-175.
18. Hastwell PW, Webster TW, Tate M, Billinton N, Lynch AM, Harvey JS, Rees RW, Walmsley RM. 2009. Analysis of 75 marketed pharmaceuticals using the GADD45a-GFP 'GreenScreen HC' genotoxicity assay. Mutagenesis 24: 455-463.
19. Hiraku Y, Kawanishi S. 1996. Oxidative damage and apoptosis induced by benzene metabolites. Cancer Res. 56: 5172-5178.
20. Jagger C, Tate M, Cahill PA, Hughes C, Knight AW, Billinton N, Walmsley RM. 2009. Assessment of the genotoxicity of S9-generated metabolites using the GreenScreen HC GADD45a-GFP assay. Mutagenesis 24: 35-50.
21. Jaju M, Jaju M, Ahuja YR. 1986. Cytogenetic effect of Neomycin on human lymphocytes in vitro. Ind. J. of Exp. Biol. 24: 595-598.
22. Knight AW, Birrel L, Walmsley RM. 2009a. Development and validation of a higher throughput screening approach to genotoxicity testing using the GADD45a-GFP GreenScreen HC assay. J. Biochem. Scr. 14: 16-30.
23. TM chemicals. Regul. Toxicol. Pharma. 55: 188-199.
24. Kirkland D, Aardema M, Henderson L, Müller L. 2005. Evaluation of the ability of a battery of three in vitro genotoxicity tests to discriminate rodent carcinogens and non-carcinogens I. Sensitivity, specificity and relative predictivity. Mutat. Res. 584: 1-256.
25. Kirkland D, Reeve L, Gatehouse D, Vanparys P. 2011. A core in vitro genotoxicity battery comprising the Ames test plus the in vitro micronucleus test is sufficient to detect rodent carcinogens and in vivo genotoxins. Mutat. Res. 721: 27-73.
26. Lynch AM, Robinson SA, Wilcox P, Smith MD, Kleinman M, Jiang K, Rees RW. 2008. Cyclohemixide and disulfoton are positive in the photoclastogenicity assay but not absorb UV irradiation: another example of pseudophotoclastogenicity? Mutagenesis 23: 111-118.
27. Lynch A, Sasaki JC, Elespuru R, Jacobson-Kram D, Thybaud V, De Boeck M, Aardema MJ, Aubrecht J, Benz RD, Dertinger S, Douglas GR, White PA, Escobar PA, Fornace A Jr, Honma M, Naven RT, Rusling JF, Schiest RH, Walmsley RM, Yamamura E, van Benthem J, Kim JH. 2011. New and emerging technologies for genetic toxicity testing. Environ. Mol. Mutagen. 52: 205-223.
28. Maron DM, Ames BN. 1983. Revised methods for the Salmonella mutagenicity test. Mutat. Res. 113: 173-215.
29. McCann J, Choi E, Yamazaki E, Ames BN. 1975. Detection of carcinogens as mutagens in the Salmonella/microsome test: Assay of 300 chemicals. Proc. Nat. Acad. Sci. USA 78: 5135-5139.
30. McCarroll NE, Protzel A, Ioannou Y, Stack HF, Jackson MA, Waters MD, Dearfield KL. 2002. A survey of EPA/OPP and open literature on selected pesticide chemicals III. Mutagenicity and carcinogenicity of benomyl and carbendazim. Mutat. Res. 512: 1-35.
31. McGregor DB, Brown A, Cattanach P, Edwards I, McBride D, Caspary WJ. 1988. Response of the L5178Y tk+/tk- assay II: 18 coded chemicals. Env. Mol. Mutagen. 11: 91-118.
32. Meintières S, Biola A, Pallardy M, Marzin, D. 2003. Using CTLL-2 and CTLL-2 bcl2 cells to avoid interference by apoptosis in the in vitro micronucleus test. Env. Mol. Mutagen. 41: 14-27.
33. Moore MM, Honma M, Clements J, Bolcsfoldi G, Cifone M, Delongchamp R, Fellows M, Gollapudi B, Jenkinson P, Kirby P, Kirchner S, Muster W, Myhr B, O'Donovan M, Oliver J, Omori T, Ouldelhkim MC, Pant K, Preston R, Riach C, San R, Stankowski LF Jr, Thakur A, Wakuri S, Yoshimura I, Mouse Lymphoma Assay Workgroup. 2003. Mouse lymphoma thymidine kinase gene mutation assay: International Workshop on Genotoxicity Tests Workgroup report- Plymouth, UK 2002. Mutat. Res. 540: 127-140.
34. Moore MM, Honma M, Clements J, Bolcsfoldi G, Burlinson B, Cifone M, Clarke J, Delongchamp R, Durward R, Fellows M, Gollapudi B, Hou S, Jenkinson P, Lloyd M, Majeska J, Myhr B, O'Donovan M, Omori T, Riach C, San R, Stankowski LF Jr, Thakur AK, Van Goethem F, Wakuri S, Yoshimura I. 2006. Mouse lymphoma thymidine kinase gene mutation assay: follow-up meeting of the International Workshop on Genotoxicity Testing - Aberdeen, Scotland, 2003 - assay acceptance criteria, positive controls, and data evaluation. Environ. Mol. Mutagen. 47: 1-5.
35. Mudry MD, Carballo M, Labal de Vinuesa M, Conzalez Cid M, Larripa I. 1994. Mutagenic bioassay of certain pharmacological drugs: III. Metronidazole (MTZ). Mutat. Res. 305: 127-132.
36. Nesslany F, Marzin D. 1999. A micromethod for the in vitro micronucleus assay. Mutagenesis 14: 403-410.
37. Ni YC, Heflich RH, Kadlubar FF, Fu PP. 1987. Mutagenicity of nitrofurans in Salmonella typhimurium TA98, TA98NR and TA98/1, 8-DNP6. Mutat. Res. 192: 15-22.
38. Olaharski A, Albertini S, Kirchner S, Plats S, Uppal H, Lin H, Kolaja K. 2009. Evaluation of the GreenScreen GADD45a-GFP indicator assay with non-proprietary and proprietary compounds. Mutat. Res. 672: 10-16.
39. Quillardet P, Hofnung M. 1993. The SOS chromotest: a review. Mutat. Res. 297: 235-279.
40. Scott D, Galloway SM, Marshall RR, Ishidate M Jr, Brusick D, Ashby J, Myhr BC. 1991. International Commission for protection against environmental mutagens and carcinogens. Genotoxicity under extreme culture conditions. A report from ICPEMC task group 9. Mutat. Res. 257: 147-204.
41. Siafakas AR, Richardson DR. 2009. Growth arrest and DNA damage-45 alpha (GADD45α). Int. J. Biochem. Cell B. 41: 986-989.
42. Singh H, Singh JR, Dhillon VS, Bali D, Paul L. 1994. In vitro and in vivo genotoxicity evaluation of hormonal drugs. II Dexamethasone. Mutat. Res. 308: 89-97.
43. Snyder RD, Green JW. 2001. A review of the genotoxicity of marketed pharmaceuticals. Mutat. Res. 488: 151-169.
44. Stich HF, Rosin MP, Wu CH, Powrie WD. 1981. The action of transition metals on the genotoxicity of simple phenols, phenolic acids and cinnamic acids. Cancer Lett. 14: 251-260.
45. van Hoeven-Arentzen PH. 1996. Carbaryl (Pesticide residues in food: 1996 evaluations Part II Toxicological). IPCS INCHEM evaluation available at: http://www.inchem.org/documents/jmpr/jmpmono/v96pr02.htm [accessed on 16 April 2012].
46. von Schulz R, Schwanitz G, Winterhoff H. 1982. Untersuchungen zur mutagenen und klastogenen Wirkung von Resorcin. Arzneim. -Forsch. 32: 533-536.
47. Yin F, Bruemmer D, Blaschke F, Hsueh WA, Law RE, Herle AJ. 2004. Signaling pathways involved in induction of GADD45 gene expression and apoptosis by troglitazone in human MCF-7 breast carcinoma cells. Oncogene 23: 4614-4623.