Development and validation of a higher throughput screening approach to genotoxicity testing using the GADD45a-GFP GreenScreen HC assay

Journal of Biomolecular Screening

Volumn 14, Issue 1, 2009, Pages 16-30

  Knight, Andrew W ^a   Birrell, Louise ^a   Walmsley, Richard M ^a,b  

^a Gentronix Ltd   (United Kingdom)

^b UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

GADD45a; Genotoxicity; Green fluorescent protein; GreenScreen HC; High throughput screening; LOPAC

Indexed keywords

1 (2 HYDROXY 5 TRIFLUOROMETHYLPHENYL) 5 TRIFLUOROMETHYL 2(3H) BENZIMIDAZOLONE; 2 (3 CHLORO 4 HYDROXYANILINO) 3 (2 NITROPHENYL)MALEIMIDE; 2 BENZHYDRYL 3 (2 IODOBENZYLAMINO)QUINUCLIDINE; 2 CHLORO 5 NITROBENZANILIDE; 2 METHOXYESTRADIOL; 2 MORPHOLINO 8 PHENYLCHROMONE; 3 (1 ADAMANTYL) 1 AMINOMETHYL 3,4 DIHYDRO 5,6 DIHYDROXY 1H 2 BENZOPYRAN; 3 [3,5 BIS(TRIFLUOROMETHYL)BENZYLOXY] 2 PHENYLPIPERIDINE; 3 [4 (3 CHLOROPHENYL) 1 PIPERAZINYL] 1,1 DIPHENYL 2 PROPANOL; 3,5 DI TERT BUTYL ALPHA CYANO 4 HYDROXYTHIOCINNAMAMIDE; 4 ETHYL 6,7 DIMETHOXY BETA CARBOLINE 3 CARBOXYLIC ACID METHYL ESTER; 8 CYCLOPENTYL 1,3 DIPROPYLXANTHINE; 9 CHLORO 2 (2 FURYL) 5,6 DIHYDRO 5 IMINO 1,2,4 TRIAZOLO[2,3 C]QUINAZOLINE; AG 494; AG 528; AG 555; AG 698; AG 835; AMINOPTERIN; AMSACRINE; ANCITABINE; AZACITIDINE; CAFFEIC ACID PHENETHYL ESTER; CAMPTOTHECIN; CGP 74514A; CHLORAMBUCIL; CHLOROETHYLCLONIDINE; CYTOSINE 1 BETA DEXTRO ARABINOFURANOSIDE; CYTOSINE DERIVATIVE; DIHYDREXIDINE; DIPHENYLIODONIUM SALT; ETOPOSIDE; FLUOROURACIL; FLUPENTIXOL; GREEN FLUORESCENT PROTEIN; GROWTH ARREST AND DNA DAMAGE INDUCIBLE PROTEIN 45; GROWTH ARREST AND DNA DAMAGE INDUCIBLE PROTEIN 45ALPHA; ISOPRENALINE; L 741626; LIDOCAINE; LOPERAMIDE; LY 367265; MELPHALAN; METHOTREXATE; MJ 33; N BENZYL 2 CYANO 3 (3,4 DIHYDROXYPHENYL)ACRYLAMIDE; NIMUSTINE; PROADIFEN; SKF 83565; UNCLASSIFIED DRUG; UNINDEXED DRUG; XK 469;

ACCURACY; ARTICLE; AUTOFLUORESCENCE; CARCINOGENESIS; CARCINOGENICITY; CHROMOSOME DAMAGE; CLASSIFICATION; COMPUTER PROGRAM; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOTOXICITY; DILUTION; DNA MODIFICATION; DRUG DETERMINATION; GENOTOXICITY; HAZARD ASSESSMENT; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; MUTAGENICITY; PHYSICAL CHEMISTRY; PRIORITY JOURNAL; PROTEIN DETERMINATION; SOLUBILITY; STRUCTURE ACTIVITY RELATION; VALIDATION PROCESS;

CELL LINE; COMBINATORIAL CHEMISTRY TECHNIQUES; COMPUTER SIMULATION; DRUG EVALUATION, PRECLINICAL; GREEN FLUORESCENT PROTEINS; HUMANS; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; MOLECULAR STRUCTURE; MUTAGENICITY TESTS;

EID: 58849156016     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057108327065     Document Type: Article

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