In vivo measurement of the acetylation state of sirtuin substrates as a proxy for sirtuin activity

Methods in Molecular Biology

Volumn 1077, Issue , 2013, Pages 217-237

  Dominy, John ^a,b   Puigserver, Pere ^a,b   Cantó, Carles ^c  

^a DANA FARBER CANCER INSTITUTE   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

^c NESTLÉ RESEARCH CENTER   (Switzerland)

Author keywords

Acetylation; Homogenization; Immunoprecipitation; Ndufa9; PGC 1 ; Sirtuins; Transfection; Western blot

Indexed keywords

HISTONE ACETYLTRANSFERASE GCN5; SIRTUIN 1; SIRTUIN 3;

ACETYLATION; ANIMAL CELL; ANIMAL CELL CULTURE; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; ELECTROPORATION; ENZYME ACTIVITY; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; IMMUNOBLOTTING; IMMUNOPRECIPITATION; IN VIVO STUDY; NONHUMAN; OSTEOSARCOMA CELL; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; WESTERN BLOTTING;

ACETYLATION; ANIMALS; BONE NEOPLASMS; CELLS, CULTURED; ELECTRON TRANSPORT COMPLEX I; HUMANS; IMMUNOBLOTTING; IMMUNOPRECIPITATION; LYSINE; MICE; MUSCLE, SKELETAL; OSTEOSARCOMA; PROTEIN PROCESSING, POST-TRANSLATIONAL; SIRTUIN 1; SIRTUIN 3; TRANSCRIPTION FACTORS;

EID: 84884178933     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-637-5_15     Document Type: Article

References (15)

1. Houtkooper RH, Pirinen E, Auwerx J (2012) Sirtuins as regulators of metabolism and healthspan. Nat Rev Mol Cell Biol 13(4):225-238. doi: 10.1038/nrm3293, pii: nrm3293
2. Gerhart-Hines Z, Dominy JE Jr, Blattler SM, Jedrychowski MP, Banks AS, Lim JH, Chim H, Gygi SP, Puigserver P (2011) The cAMP/PKA pathway rapidly activates SIRT1 to promote fatty acid oxidation independently of changes in NAD(+). Mol Cell 44(6):851-863. doi: 10.1016/j.molcel.2011.12.005, pii: S1097-2765(11)00944-0
3. Canto C, Auwerx J (2012) Targeting sirtuin 1 to improve metabolism: all you need is NAD+? Pharmacol Rev 64(1):166-187. doi: 10.1124/pr.110.003905, pii: pr.110.003905
4. Rodgers JT, Lerin C, Haas W, Gygi SP, Spiegelman BM, Puigserver P (2005) Nutrient control of glucose homeostasis through a complex of PGC-1alpha and SIRT1. Nature 434(7029):113-118. doi: 10.1038/nature03354, pii: nature03354
5. Ahn BH, Kim HS, Song S, Lee IH, Liu J, Vassilopoulos A, Deng CX, Finkel T (2008) A role for the mitochondrial deacetylase Sirt3 in regulating energy homeostasis. Proc Natl Acad Sci U S A 105(38):14447-14452. doi: 10.1073/pnas.0803790105, pii: 0803790105
6. Lerin C, Rodgers JT, Kalume DE, Kim SH, Pandey A, Puigserver P (2006) GCN5 acetyltransferase complex controls glucose metabolism through transcriptional repression of PGC-1alpha. Cell Metab 3(6):429-438. doi: 10.1016/j.cmet.2006.04.013
7. Canto C, Gerhart-Hines Z, Feige JN, Lagouge M, Noriega L, Milne JC, Elliott PJ, Puigserver P, Auwerx J (2009) AMPK regulates energy expenditure by modulating NAD+ metabolism and SIRT1 activity. Nature 458(7241):1056-1060. doi: 10.1038/nature07813, pii: nature07813
8. Gerhart-Hines Z, Dominy JE Jr, Blattler SM, Jedrychowski MP, Banks AS, Lim JH, Chim H, Gygi SP, Puigserver P (2011) The cAMP/PKA pathway rapidly activates SIRT1 to promote fatty acid oxidation independently of changes in NAD(+). Mol Cell 44(6):851-863. doi: 10.1016/j.molcel.2011.12.005
9. Nin V, Escande C, Chini CC, Giri S, Camacho-Pereira J, Matalonga J, Lou Z, Chini EN (2012) Role of deleted in breast cancer 1 (DBC1) protein in SIRT1 deacetylase activation induced by protein kinase A and AMP-activated protein kinase. J Biol Chem 287(28):23489-23501. doi: 10.1074/jbc.M112.365874
10. Canto C, Houtkooper RH, Pirinen E, Youn DY, Oosterveer MH, Cen Y, Fernandez-Marcos PJ, Yamamoto H, Andreux PA, Cettour-Rose P, Gademann K, Rinsch C, Schoonjans K, Sauve AA, Auwerx J (2012) The NAD(+) precursor nicotinamide riboside enhances oxidative metabolism and protects against high-fat dietinduced obesity. Cell Metab 15(6):838-847. doi: 10.1016/j.cmet.2012.04.022, pii: S1550-4131(12)00192-1
11. Verdin E, Hirschey MD, Finley LW, Haigis MC (2010) Sirtuin regulation of mitochondria: energy production, apoptosis, and signaling. Trends Biochem Sci 35(12):669-675. doi: 10.1016/j.tibs.2010.07.003, pii: S0968-0004(10)00135-0
12. Lombard DB, Alt FW, Cheng HL, Bunkenborg J, Streeper RS, Mostoslavsky R, Kim J, Yancopoulos G, Valenzuela D, Murphy A, Yang Y, Chen Y, Hirschey MD, Bronson RT, Haigis M, Guarente LP, Farese RV Jr, Weissman S, Verdin E, Schwer B (2007) Mammalian Sir2 homolog SIRT3 regulates global mitochondrial lysine acetylation. Mol Cell Biol 27(24):8807-8814. doi: 10.1128/MCB.01636-07, pii: MCB.01636-07
13. Fernandez-Marcos PJ, Jeninga EH, Canto C, Harach T, de Boer VC, Andreux P, Moullan N, Pirinen E, Yamamoto H, Houten SM, Schoonjans K, Auwerx J (2012) Muscle or liver-specific Sirt3 deficiency induces hyperacetylation of mitochondrial proteins without affecting global metabolic homeostasis. Sci Rep 2:425. doi: 10.1038/srep00425
14. Timchenko NA, Wilde M, Darlington GJ (1999) C/EBPalpha regulates formation of S-phase-specific E2F-p107 complexes in livers of newborn mice. Mol Cell Biol 19(4): 2936-2945
15. Lagouge M, Argmann C, Gerhart-Hines Z, Meziane H, Lerin C, Daussin F, Messadeq N, Milne J, Lambert P, Elliott P, Geny B, Laakso M, Puigserver P, Auwerx J (2006) Resveratrol improves mitochondrial function and protects against metabolic disease by activating SIRT1 and PGC-1alpha. Cell 127(6):1109-1122. doi: 10.1016/j.cell.2006.11.013, pii: S0092-8674(06)01428-0