Purification of a novel protease enzyme from kesinai plant (Streblus asper) leaves using a surfactant-salt aqueous micellar two-phase system: A potential low cost source of enzyme and purification method

European Food Research and Technology

Volumn 237, Issue 4, 2013, Pages 601-608

  Amid, Mehrnoush ^a   Abd Manap, Mohd Yazid ^a   Shuhaimi, Mustafa ^a  

^a UNIVERSITY PUTRA MALAYSIA   (Malaysia)

Author keywords

Kesinai leaves; New aqueous micellar phase solution; Partition behaviour; Phase recycling; Protease enzyme; Purification

Indexed keywords

AQUEOUS MICELLAR TWO-PHASE SYSTEMS; HYDROPHOBIC INTERACTIONS; KESINAI LEAVES; MICELLAR PHASE; PARTITIONING BEHAVIOURS; PROTEASE ENZYME; PURIFICATION FACTORS; SURFACTANT-RICH PHASE;

AMINO ACIDS; ENZYMES; HYDROPHOBICITY; IONIC STRENGTH; MICELLES; PLANTS (BOTANY); RECYCLING; SURFACE ACTIVE AGENTS;

PURIFICATION;

STREBLUS ASPER;

EID: 84883773980     PISSN: 14382377     EISSN: 14382385     Source Type: Journal    
DOI: 10.1007/s00217-013-2037-3     Document Type: Article

References (42)

1. Senthilkumar S, Ramasamy D, Subramanian S (2006) Isolation and partial characterisation of milk-clotting aspartic protease from Streblus asper. Int J Food Sci 12: 103-109.
2. Tomar R, Kumar R, Jagannadham MV (2008) A stable serine protease, wrightin, from the latex of the plant Wrightia tinctoria (roxb.) R. br.: purification and biochemical properties. J Agric Food Chem 56: 1479-1487.
3. Rastogi S, Kulshreshtha DK, Rawat AKS (2006) Streblus asper Lour. (Shakhotaka): a review of its chemical, pharmacological and ethnomedicinal properties. J Evid Based Complement Altern Med 3: 217-222.
4. Taweechaisupapong S, Choopan T, Singhara S, Chatrchaiwiwatana S, Wongkham S (2005) In vitro inhibitory effect of Streblus asper leaf-extract on adhesion of Candida albicans to human buccal epithelial cells. J Ethnopharmacol 96: 221-226.
5. Bermejo R, Ruiz E, Acien FG (2007) Recovery of B-phycoerythrin using expanded bed adsorption chromatography: scale-up of the process. J Enzyme Microb Technol 40: 927-933.
6. Malpiedi LP, Pico G, Nerli B (2008) Features of partitioning pattern of two pancreatic enzymatic precursors: trypsinogen and chymotrypsinogen in polyethyleneglycol-sodium citrate aqueous biphasic systems. J Chromatogr B 870: 1-7.
7. Rahimpour F, Feyzi F, Machsoudi S, Hatti-Kaulr R (2006) Purification of plasmid DNA with polymer-salt aqueous two-phase system: optimization using response surface methodology. J Biotechnol Bioeng 95: 627-637.
8. Tianwei T, Qing H, Qiang L (2002) Purification of glycyrrhizin from Glycyrrhiza uralensis Fisch with ethanol/phosphate aqueous two phase system. J Biotechnol Lett 24: 1417-1420.
9. Mashayekhi F, Meyer AS, Shigi SA, Nguyen V, Kamei DT (2009) Concentration of mammalian genomic DNA using two-phase aqueous micellar systems. J Biotechnol Bioeng 102: 1613-1623.
10. Mazzola PG, Lopes AM, Hasmann FA et al (2008) Liquid-liquid extraction of biomolecules: an overview and update of the main techniques. J Chem Technol Biotech 83: 143-157.
11. Bi W, Tian M, Row KH (2011) Extraction and concentration of tanshinones in Salvia miltiorrhiza Bunge by task-specific non-ionic surfactant assistance. J Food Chem 126: 1985-1990.
12. Tani H, Kamidate T, Watanabe H (1998) Aqueous micellar two-phase systems for protein separation. J Anal Sci 14: 875-888.
13. Quina FH, Hinze WL (1999) Surfactant-mediated cloud point extractions: an environmentally benign alternative separation approach. J Ind Eng Chem Res 38: 4150-4168.
14. Patel K, Bharatiya B, Kadam Y, Bahadur P (2010) Micellization and clouding behavior of EO-PO block copolymer in aqueous salt solutions. J Surfactants Deterg 13: 89-95.
15. Lopes AM, Pessoa A, Rangel-Yagui CO (2008) Can affinity interactions influence the partitioning of glucose-6-phosphate dehydrogenase in two-phase aqueous micellar systems. J Quím Nova 31: 998-1003.
16. Kamei DT, Wang DIC, Blankschtein D (2002) Fundamental investigation of protein partitioning in two-phase aqueous mixed (nonionic/ionic) micellar systems. J Langmuir 18: 3047-3057.
17. Becker JS, Thomas ORT, Franzreb M (2009) Protein separation with magnetic adsorbents in micellar aqueous two-phase systems. Sep Purif Technol 65: 46-53.
18. Minuth T (2000) Extraction of amphiphilic proteins using detergent-based aqueous two-phase systems. J Methods Biotech 11: 291-302.
19. Chien WO, Chin PT, Siew LH, Arbakariya A, Ibrahim S, Ling TC (2011) Primary recovery of lipase derived from Burkholderia sp. ST8 with aqueous micellar two-phase system. J Process Biochem 46: 1847-1852.
20. Jozala AF, Lopes AM, Mazzola PG, Magalhães PO, Vessoni Penna TC, Pessoa A Jr (2008) Liquid-liquid extraction of commercial and biosynthesized nisin by aqueous two-phase micellar systems. J Enzyme Microb Technol 42: 107-112.
21. Mazzola PG, Lam H, Kavoosi M, Haynes CA, Pessoa Júnior A, Vessoni Penna TC (2006) Affinity tagged green fluorescent protein (GFP) extraction from a clarified E. coli cell lysate using a two-phase aqueous micellar system. J Biotechnol Bioeng 93: 998-1004.
22. Minuth T, Thömmes J, Kula MR (1995) Extraction of cholesterol oxidase from Nocardia rhodochrous using a nonionic surfactant-based aqueous two-phase system. J Biotechnol 38: 151-164.
23. Sivars U, Tjerneld F (2000) Mechanisms of phase behaviour and protein partitioning in detergent/polymer aqueous two-phase systems for purification of integral membrane proteins. J Biochimica et Biophysica Acta 1474: 133-146.
24. Liu C, Kamei DT, King JA, Wang DIC, Blankschtein D (1998) Separation of proteins and viruses using two-phase aqueous micellar systems. J Chromatogr B 711: 127-138.
25. Santos VC, Hasmann FA, Converti A, Pessoa A (2011) Liquid-liquid extraction by mixed micellar systems: a new approach for clavulanic acid recovery from fermented broth. J Biochem Eng 56: 75-83.
26. Jozala AF, Lopes AM, Mazzola PG, Magalhães PO, Vessoni Penna TC, Pessoa A (2008) Liquid-liquid extraction of commercial and biosynthesized nisin by aqueous two-phase micellar systems. J Enzyme Microb Technol 42: 107-112.
27. Dosoretz CG, Chen HC, Grethlein HE (1990) Effect of environmental conditions on extracellular protease activity in lignolytic cultures of Phanerochaete chrysosporium. J Appl Environ Microbiol 56: 395-400.
28. Costa MJL, Cunha MT, Cabral JMS, Aires-Barros MR (2005) Scale-up of recombinant cutinase recovery by whole broth extraction with PEG-phosphate aqueous two-phase. J Biosep 9: 231-238.
29. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. J Nat 227: 680-685.
30. Blum H, Beier H, Gross HJ (1987) Improved silver staining of plant-proteins, RNA and DNA in polyacrylamide gels. J Electrophor 8: 93-99.
31. Mirjana G, Draginja M, Milica G (2006) Aqueous two-phase partitioning of xylanase produced by solid-state cultivation of Polyporus squamosus. J Process Biochem 41: 232-325.
32. Terstappen RG, Ramelmeier CA, Kula MR (1993) Protein partitioning in detergent-based aqueous two-phase systems. J Biotechnol 28: 263-275.
33. Imm JY, Kim SC (2009) Convenient partial purification of polyphenol oxidase from apple skin by cationic reversed micellar extraction. J Food Chem 113: 302-306.
34. Lianhong Y, Sun CK, Han X et al (2011) Preparative purification of bromelain (EC 3. 4. 22. 33) from pineapple fruit by high-speed counter-current chromatography using a reverse-micelle solvent system. J Food Chem 129: 925-932.
35. Nan YQ, Hao LS (2008) Salt-induced phase inversion in aqueous cationic/anionic surfactant two-phase systems. J Physic Chem B 112: 12326-12337.
36. Johansson HO, Persson J, Tjerneld F (2000) Thermoseparating water/polymer system: a novel one-polymer aqueous two-phase system for protein purification. J Biotechnol Bioeng 66: 247-257.
37. Brasher LL, Herrington KL, Kaler EW (1995) Electrostatic effects on the phase behavior of aqueous cetyltrimethylammonium bromide and sodium octyl sulfate mixtures with added sodium bromide. Langmuir 11: 4267-4277.
38. Johansson HO, Persson J, Tjerneld F (1999) Thermoseparating water/polymer system: a novel one-polymer aqueous two-phase system for protein purification. J Biotechnol Bioeng 66: 247-257.
39. Parish CR, Classon BJ, Tsagaratos J, Walker ID, Kirszbaum L, McKenzie IFC (1986) Fractionation of detergent lysates of cells by ammonium sulphate-induced phase separation. J Anal Biochem 156: 495-502.
40. Zhi W, Deng Q (2006) Purification of salvianolic acid B from the crude extract of Salvia miltiorrhiza with hydrophilic organic/salt-containing aqueous two-phase system by counter-current chromatography. J Chromatogr A 1116: 149-152.
41. Al-Hanbali O, Onwuzo NM, Rutt KJ, Dadswell CM, Moghimi SM, Hunter AC (2007) Modification of the Stewart biphasic colorimetric assay for stable and accurate quantitative determination of Pluronic and Tetronic block copolymers for application in biological systems. J Anal Biochem 361: 287-293.
42. Hemavathi AB, Umesh Hebbar H, Raghavarao KSMS (2010) Mixed reverse micellar systems for extraction and purification of β-glucosidase. J Sep Purif Technol 71: 263-268.