Coupling unbiased mutagenesis to high-throughput DNA sequencing uncovers functional domains in the Ndc80 kinetochore protein of Saccharomyces cerevisiae

Genetics

Volumn 195, Issue 1, 2013, Pages 159-170

  Tien, Jerry F ^a   Fong, Kimberly K ^a   Umbreit, Neil T ^a   Payen, Celia ^b   Zelter, Alex ^a   Asbury, Charles L ^b   Dunham, Maitreya J ^b   Davis, Trisha N ^a  

^a UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF WASHINGTON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CALPONIN; DNA; NDC80 COMPLEX;

ARTICLE; CENTROMERE; CONTROLLED STUDY; DNA SEQUENCE; FLUORESCENCE MICROSCOPY; GENE MUTATION; GENETIC SCREENING; HIGH THROUGHPUT SEQUENCING; MICROTUBULE; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; SACCHAROMYCES CEREVISIAE;

COILED COIL; HEC1; ILLUMINA; TOTAL INTERNAL REFLECTION FLUORESCENCE (TIRF);

AMINO ACID SEQUENCE; CELL CYCLE PROTEINS; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; INVERTED REPEAT SEQUENCES; KINETOCHORES; MICROTUBULE-ASSOCIATED PROTEINS; MICROTUBULES; MOLECULAR SEQUENCE DATA; MUTAGENESIS; NUCLEAR PROTEINS; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE ANALYSIS, DNA;

EID: 84883758590     PISSN: 00166731     EISSN: 19432631     Source Type: Journal    
DOI: 10.1534/genetics.113.152728     Document Type: Article

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