High-precision mapping of protein-protein interfaces: An integrated genetic strategy combining en masse mutagenesis and DNA-level parallel analysis on a yeast two-hybrid platform

Nucleic Acids Research

Volumn 35, Issue 16, 2007, Pages

  Pajunen, Maria ^a   Turakainen, Hilkka ^a   Poussu, Eini ^a   Peränen, Johan ^a   Vihinen, Mauno ^b,c   Savilahti, Harri ^a,d  

^a UNIVERSITY OF HELSINKI   (Finland)

^b UNIVERSITY OF TAMPERE   (Finland)

^c TAMPERE UNIVERSITY HOSPITAL   (Finland)

^d UNIVERSITY OF TURKU   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; PENTAPEPTIDE; PROTEIN JFC1; RAB PROTEIN; SYNAPTOTAGMIN; UNCLASSIFIED DRUG; JFC1 PROTEIN, HUMAN; MEMBRANE PROTEIN; RAB8A PROTEIN, HUMAN;

ACCURACY; ARTICLE; BACTERIOPHAGE MU; CONTROLLED STUDY; DNA DETERMINATION; FEASIBILITY STUDY; GENE INSERTION; GENE LIBRARY; GENE MAPPING; GENE MUTATION; GENETIC ASSOCIATION; GENETIC CODE; GENETIC SCREENING; IN VITRO STUDY; MUTATIONAL ANALYSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN DOMAIN; PROTEIN FOOTPRINTING; PROTEIN PROTEIN INTERACTION; SAMPLING; SEQUENCE HOMOLOGY; TWO HYBRID SYSTEM; YEAST; AMINO ACID SEQUENCE; BINDING SITE; CHEMISTRY; DNA SEQUENCE; EVALUATION; GENE EXPRESSION REGULATION; GENETICS; HUMAN; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; POLYMERASE CHAIN REACTION; PROTEIN SECONDARY STRUCTURE; PROTEIN TERTIARY STRUCTURE; SACCHAROMYCES CEREVISIAE;

AMINO ACID SEQUENCE; BINDING SITES; GENE LIBRARY; HUMANS; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, INSERTIONAL; POLYMERASE CHAIN REACTION; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; RAB GTP-BINDING PROTEINS; SACCHAROMYCES CEREVISIAE; SEQUENCE ANALYSIS, DNA; TWO-HYBRID SYSTEM TECHNIQUES;

EID: 34548743990     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gkm563     Document Type: Article

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