The yeast DASH complex forms closed rings on microtubules

Nature Structural and Molecular Biology

Volumn 12, Issue 2, 2005, Pages 138-143

  Miranda, J J L ^a   De Wulf, Peter ^b   Sorger, Peter K ^b   Harrison, Stephen C ^c  

^a HARVARD UNIVERSITY   (United States)

^b MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^c HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING PROTEIN; DASH PROTEIN; FUNGAL PROTEIN; PROTEIN SUBUNIT; UNCLASSIFIED DRUG; MULTIPROTEIN COMPLEX; SACCHAROMYCES CEREVISIAE PROTEIN;

ARTICLE; CENTROMERE; COMPLEX FORMATION; CONTROLLED STUDY; ELECTRON MICROSCOPY; ESCHERICHIA COLI; HYDRODYNAMICS; MICROTUBULE; NONHUMAN; OLIGOMERIZATION; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; STOICHIOMETRY; YEAST; CHEMISTRY; CHROMOSOME SEGREGATION; GENETICS; METABOLISM; SACCHAROMYCES CEREVISIAE;

ESCHERICHIA COLI; SACCHAROMYCES CEREVISIAE;

CHROMOSOME SEGREGATION; KINETOCHORES; MICROSCOPY, ELECTRON; MICROTUBULES; MULTIPROTEIN COMPLEXES; PROTEIN SUBUNITS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

EID: 15444372660     PISSN: 15459993     EISSN: 15459985     Source Type: Journal    
DOI: 10.1038/nsmb896     Document Type: Article

References (35)

1. McAinsh, A.D., Tytell, J.D. & Sorger, P.K. Structure, function, and regulation of budding yeast kinetochores. Annu. Rev. Cell Dev. Biol. 19, 519-539 (2003).
2. Hofmann, C. et al. Saccharomyces cerevisiae Duo1p and Dam1p, novel proteins involved in mitotic spindle function. J. Cell Biol. 143, 1029-1040 (1998).
3. Jones, M.H., Bachant, J.B., Castillo, A.R., Giddings, T.H. Jr. & Winey, M. Yeast Dam1p is required to maintain spindle integrity during mitosis and interacts with the Mps1p kinase. Mol. Biol. Cell 10, 2377-2391 (1999).
4. Cheeseman, I.M., Enquist-Newman, M., Muller-Reichert, T., Drubin, D.G. & Barnes, G. Mitotic spindle integrity and kinetochore function linked by the Duo1p/Dam1p complex. J. Cell Biol. 152, 197-212 (2001).
5. Jones, M.H., He, X., Giddings, T.H. & Winey, M. Yeast Dam1p has a role at the kinetochore in assembly of the mitotic spindle. Proc. Natl. Acad. Sci. USA 98, 13675-13680 (2001).
6. Cheeseman, I.M. et al. Implication of a novel multiprotein Dam1p complex in outer kinetochore function. J. Cell Biol. 155, 1137-1145 (2001).
7. Janke, C., Ortiz, J., Tanaka, T.U., Lechner, J. & Schiebel, E. Four new subunits of the Dam1-Duo1 complex reveal novel functions in sister kinetochore biorientation. EMBO J. 21, 181-193 (2002).
8. Li, Y. et al. The mitotic spindle is required for loading of the DASH complex onto the kinetochore. Genes Dev. 16, 183-197 (2002).
9. Cheeseman, I.M. et al. Phospho-regulation of kinetochore-microtubule attachments by the Aurora kinase Ipl1p. Cell 111, 163-172 (2002).
10. Li, J., Li, Y. & Elledge, S.J. Genetic analysis of the kinetochore DASH complex reveals an antagonistic relationship with the ras/protein kinase A pathway and a novel subunit required for Ask1 association. Mol. Cell. Biol. 25, 767-778 (2005).
11. He, X., Rines, D.R., Espelin, C.W. & Sorger, P.K. Molecular analysis of kinetochore-microtubule attachment in budding yeast. Cell 106, 195-206 (2001).
12. Enquist-Newman, M. et al. Dad1p, third component of the Duo1p/Dam1p complex involved in kinetochore function and mitotic spindle integrity. Mol. Biol. Cell 12, 2601-2613 (2001).
13. Tanaka, T.U. et al. Evidence that the Ipl1-Sli15 (Aurora kinase-INCENP) complex promotes chromosome bi-orientation by altering kinetochore-spindle pole connections. Cell 108, 317-329 (2002).
14. Shang, C. et al. Kinetochore protein interactions and their regulation by the Aurora kinase Ipl1p. Mol. Biol. Cell 14, 3342-3355 (2003).
15. Hill, T.L. Theoretical problems related to the attachment of microtubules to kinetochores. Proc. Natl. Acad. Sci. USA 82, 4404-4408 (1985).
16. Tan, S. A modular polycistronic expression system for overexpressing protein complexes in Escherichia coli. Protein Expr. Purif. 21, 224-234 (2001).
17. Rial, D.V. & Ceccarelli, E.A. Removal of DnaK contamination during fusion protein purifications. Protein Expr. Purif. 25, 503-507 (2002).
18. Gragerov, A., Zeng, L., Zhao, X., Burkholder, W. & Gottesman, M.E. Specificity of DnaK-peptide binding. J. Mol. Biol. 235, 848-854 (1994).
19. Jacobs, M., Bennett, P.M. & Dickens, M.J. Duplex microtubule is a new form of tubulin assembly induced by polycations. Nature 257, 707-709 (1975).
20. Behnke, O. An outer component of microtubules. Nature 257, 709-710 (1975).
21. Erickson, H.P. & Voter, W.A. Polycation-induced assembly of purified tubulin. Proc. Natl. Acad. Sci. USA 73, 2813-2817 (1976).
22. Erickson, H.P. Facilitation of microtubule assembly by polycations. In Cell Motility Vol. 3 (eds. Goldman, R., Pollard, T. & Rosenbaum, J.) 1069-1080 (Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 1976).
23. Al-Bassam, J., Ozer, R.S., Safer, D., Halpain, S. & Milligan, R.A. MAP2 and tau bind longitudinally along the outer ridges of microtubule protofilaments. J. Cell Biol. 157, 1187-1196 (2002).
24. Kikkawa, M., Ishikawa, T., Wakabayashi, T. & Hirokawa, N. Three-dimensional structure of the kinesin head-microtubule complex. Nature 376, 274-277 (1995).
25. Hoenger, A. et al. A new look at the microtubule binding patterns of dimeric kinesins. J. Mol. Biol. 297, 1087-1103 (2000).
26. Sawaya, M.R., Guo, S., Tabor, S., Richardson, C.C. & Ellenberger, T. Crystal structure of the helicase domain from the replicative helicase-primase of bacteriophage T7. Cell 99, 167-177 (1999).
27. Singleton, M.R., Sawaya, M.R., Ellenberger, T. & Wigley, D.B. Crystal structure of T7 gene 4 ring helicase indicates a mechanism for sequential hydrolysis of nucleotides. Cell 101, 589-600 (2000).
28. Hinshaw, J.E. & Schmid, S.L. Dynamin self-assembles into rings suggesting a mechanism for coated vesicle budding. Nature 374, 190-192 (1995).
29. Ghaemmaghami, S. et al. Global analysis of protein expression in yeast. Nature 425, 737-741 (2003).
30. Moores, C.A. et al. Mechanism of microtubule stabilization by doublecortin. Mol. Cell 14, 833-839 (2004).
31. Hingorani, M.M. & O'Donnell, M. A tale of toroids in DNA metabolism. Nat. Rev. Mol. Cell Biol. 1, 22-30 (2000).
32. Horiike, K., Tojo, H., Yamano, T. & Nozaki, M. Interpretation of the Stokes radius of macromolecules determined by gel filtration chromatography. J. Biochem. 93, 99-106 (1983).
33. Salmon, E.D., Saxton, W.M., Leslie, R.J., Karow, M.L. & McIntosh, J.R. Diffusion coefficient of fluorescein-labeled tubulin in the cytoplasm of embryonic cells of a sea urchin: video image analysis of fluorescence redistribution after photobleaching. J. Cell Biol. 99, 2157-2164 (1984).
34. Sorger, P.K. & Pelham, H.R. Purification and characterization of a heat-shock element binding protein from yeast. EMBO J. 6, 3035-3041 (1987).
35. Russell, I.D., Grancell, A.S. & Sorger, P.K. The unstable F-box protein p58-Ctf13 forms the structural core of the CBF3 kinetochore complex. J. Cell Biol. 145, 933-950 (1999).