Rapid and accurate detection of viable Escherichiacoli O157: H7 in milk using a combined IMS, sodium deoxycholate, PMA and real-time quantitative PCR process

Food Control

Volumn 36, Issue 1, 2014, Pages 119-125

  Wang, Lijun ^a   Li, Ping ^a   Zhang, Zhihong ^a   Chen, Qi ^a   Aguilar, Zoraida P ^b   Xu, Hengyi ^a   Yang, Lin ^a   Xu, Feng ^a   Lai, Weihua ^a   Xiong, Yonghua ^a   Wei, Hua ^a  

^a NANCHANG UNIVERSITY   (China)

^b Zystein LLC   (United States)

Author keywords

Escherichiacoli O157:H7; Propidium monoazide; QPCR; Sodium deoxycholate

Indexed keywords

EID: 84883644027     PISSN: 09567135     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.foodcont.2013.08.011     Document Type: Article

References (31)

1. Applied Biosystems Application note http://www3.appliedbiosystems.com/cms/groups/mcb_marketing/documents/generaldocuments/cms_053906.pdf.
2. Bae S., Wuertz S. Survival of host-associated bacteroidales cells and their relationship with Enterococcus spp., Campylobacter jejuni, Salmonella enterica serovar Typhimurium, and adenovirus in freshwater microcosms as measured by propidium monoazide-quantitative PCR. Applied and Environmental Microbiology 2012, 78:922-932.
3. Bickley J., Short J., McDowell D., Parkes H. Polymerase chain reaction (PCR) detection of Listeria monocytogenes in diluted milk and reversal of PCR inhibition caused by calcium ions. Letters in Applied Microbiology 1996, 22:153-158.
4. Bioterrorism Agents/Diseases CDC-Centers for Disease Control and Prevention 2004, [online] Available. http://www.bt.cdc.gov/agent/agentlist-category.asp.
5. Carey C.M., Kostrzynska M., Thompson S. Escherichia coli O157:H7 stress and virulence gene expression on romaine lettuce using comparative real-time PCR. Journal of Microbiological Methods 2009, 77:235-242.
6. Cheville A., Arnold K., Buchrieser C., Cheng C., Kaspar C. RpoS regulation of acid, heat, and salt tolerance in Escherichia coli O157:H7. Applied and Environmental Microbiology 1996, 62:1822-1824.
7. Elizaquível P., Gabaldón J., Aznar R. Quantification of Salmonella spp., Listeria monocytogenes and Escherichia coli O157:H7 in non-spiked food products and evaluation of real-time PCR as a diagnostic tool in routine food analysis. Food Control 2011, 22:158-164.
8. Elizaquível P., Sánchez G., Aznar R. Quantitative detection of viable foodborne E.coli O157:H7, Listeria monocytogenes and Salmonella in fresh-cut vegetables combining propidium monoazide and real-time PCR. Food Control 2011, 25:704-708.
9. Elizaquível P., Sánchez G., Selma M., Aznar R. Application of propidium monoazide-qPCR to evaluate the ultrasonic inactivation of E.coli O157:H7 in fresh-cut vegetable wash water. Food Microbiology 2012, 30:316-320.
10. Frenzen P.D., Drake A., Angulo F.J. Economic cost of illness due to Escherichia coli O157 infections in the United States. Journal of Food Protection 2005, 68:2623-2630.
11. Griffin P.M., Tauxe R.V. The epidemiology of infections caused by Escherichia coli O157:H7, other enterohemorrhagic E.coli, and the associated hemolytic uremic syndrome. Epidemiologic Reviews 1991, 13:60-98.
12. Juneja V.K., Snyder O.P., Marmer B.S. Thermal destruction of Escherichia coli O157:H7 in beef and chicken: determination of D-and Z-values. International Journal of Food Microbiology 1997, 35:231-237.
13. Lee J.L., Levin R.E. Discrimination of viable and dead Vibrio vulnificus after refrigerated and frozen storage using EMA, sodium deoxycholate and real-time PCR. Journal of Microbiological Methods 2009, 79:184-188.
14. Lestari S.I., Han F., Wang F., Ge B. Prevalence and antimicrobial resistance of Salmonella serovars in conventional and organic chickens from Louisiana retail stores. Journal of Food Protection 2009, 72:1165-1172.
15. Løvdal T., Hovda M.B., Björkblom B., Møller S.G. Propidium monoazide combined with real-time quantitative PCR underestimates heat-killed Listeria innocua. Journal of Microbiological Methods 2011, 85:164-169.
16. Malorny B., Tassios P.T., Rådström P., Cook N., Wagner M., Hoorfar J. Standardization of diagnostic PCR for the detection of foodborne pathogens. International Journal of Food Microbiology 2003, 83:39-48.
17. Martinon A., Cronin U., Quealy J., Stapleton A., Wilkinson M. Swab sample preparation and viable real-time PCR methodologies for the recovery of Escherichia coli, Staphylococcus aureus or Listeria monocytogenes from artificially contaminated food processing surfaces. Food Control 2012, 24:86-94.
18. Nair S., Finkel S.E. Dps protects cells against multiple stresses during stationary phase. Journal of Bacteriology 2004, 186:4192-4198.
19. Ngwa G., Schop R., Weir S., León-Velarde C., Odumeru J. Detection and enumeration of E.coli O157:H7 in water samples by culture and molecular methods. Journal of Microbiological Methods 2012, 92:164-172.
20. Nocker A., Cheung C.Y., Camper A.K. Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cells. Journal of Microbiological Methods 2006, 67:310-320.
21. Peter F., Stephen D.W., Jinneman K. Diarrheagenic Escherichia coli. Bacteriological analytical manual 2011, FDA, Online. http://www.fda.gov/food/scienceresearch/laboratorymethods/bacteriologicalanalyticalmanualbam/ucm070080.htm.
22. Romero C., Pardo M., Grillo M., Diaz R., Blasco J., Lopez-Goñi I. Evaluation of PCR and indirect enzyme-linked immunosorbent assay on milk samples for diagnosis of brucellosis in dairy cattle. Journal of Clinical Microbiology 1995, 33:3198-3200.
23. Shannon K., Lee D.Y., Trevors J., Beaudette L. Application of real-time quantitative PCR for the detection of selected bacterial pathogens during municipal wastewater treatment. Science of the Total Environment 2007, 382:121-129.
24. Smith M. Survival of E.coli and Salmonella after chilling and freezing in liquid media. Journal of Food Science 2006, 60:509-512.
25. Wang L.J., Li P., Yang Y.J., Xu H., Aguilar Z.P., Xu H.Y., et al. Development of an IMS-PMA-PCR assay with internal amplification control for rapid and sensitive detection of viable Escherichia coli O157:H7 in milk. International Dairy Journal 2013, 10.1016/j.idairyj.2013.07.006.
26. Wright J.R., Sumner S.S., Hackney C.R., Pierson M.D., Zoecklein B.D. Efficacy of ultraviolet light for reducing Escherichia coli O157:H7 in unpasteurized apple cider. Journal of Food Protection 2000, 63:563-567.
27. Yang X., Badoni M., Gill C.O. Use of propidium monoazide and quantitative PCR for differentiation of viable Escherichia coli from E.coli killed by mild or pasteurizing heat treatments. Food Microbiology 2011, 28:1478-1482.
28. Yang Y.J., Wan C.X., Xu H.Y., Lai W.H., Xiong Y.,H., Xu F., et al. Development of a multiplexed PCR assay combined with propidium monoazide treatment for rapid and accurate detection and identification of three viable Salmonella enteric serovars. Food Control 2012, 28:456-462.
29. Yang Y.J., Xu F., Xu H.Y., Aguilar Z.P., Niu R.J., Yuan Y., et al. Magnetic nano-beads based separation combined with propidium monoazide treatment and multiplex PCR assay for simultaneous detection of viable Salmonella typhimurium, Escherichia coli O157:H7 and Listeria monocytogenes in food products. Food Microbiology 2013, 34:418-424.
30. Zhang Z.H., Wang L.J., Xu H.Y., Aguilar Z.P., Liu C.W., Gan B. Detection of non-emetic and emetic Bacillus cereus by propidium monoazide multiplex PCR (PMA-mPCR) with internal amplification control. Food Control 2013, 10.1016/j.foodcont.2013.07.035.
31. Zhu R.G., Li T.P., Jia Y.F., Song L.F. Quantitative study of viable Vibrio parahaemolyticus cells in raw seafood using propidium monoazide in combination with quantitative PCR. Journal of Microbiological Methods 2012, 90:262-266.