Detection of non-emetic and emetic Bacillus cereus by propidium monoazide multiplex PCR (PMA-mPCR) with internal amplification control

Food Control

Volumn 35, Issue 1, 2014, Pages 401-406

  Zhang, Zhihong ^a   Wang, Lijun ^a   Xu, Hengyi ^a   Aguilar, Zoraida P ^b   Liu, Chengwei ^c   Gan, Bei ^d   Xiong, Yonghua ^a   Lai, Weihua ^a   Xu, Feng ^a   Wei, Hua ^a  

^a NANCHANG UNIVERSITY   (China)

^b Zystein LLC   (United States)

^c Institute of Nutrition and Food Safety   (China)

^d Jiangxi Provincial Product Quality Supervision Testing College   (China)

Author keywords

Bacillus cereus; CesB gene; Detection; IAC; Multiplex PCR; Propidium monoazide

Indexed keywords

EID: 84883009256     PISSN: 09567135     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.foodcont.2013.07.035     Document Type: Article

References (36)

1. Àlvarez G., González M., Isabal S., Blanc V., Berríos R.L. Method to quantify live and dead cells in multi-species oral biofilm by real-time PCR with propidium monoazide. AMB Express 2013, 3(1):1-8.
2. Apetroaie-Constantin C., Shaheen R., Andrup L., Smidt L., Rita H., Salkinoja-Salonen M. Environment driven cereulide production by emetic strains of Bacillus cereus. International Journal of Food Microbiology 2008, 127(1):60-67.
3. Ceuppens S., Boon N., Rajkovic A., Heyndrickx M., Van de Wiele T., Uyttendaele M. Quantification methods for Bacillus cereus vegetative cells and spores in the gastrointestinal environment. Journal of Microbiological Methods 2010, 83(2):202-210.
4. Chang B., Sugiyama K., Taguri T., Amemura-Maekawa J., Kura F., Watanabe H. Specific detection of viable Legionella cells by combined use of photoactivated ethidium monoazide and PCR/real-time PCR. Applied and Environmental Microbiology 2009, 75(1):147-153.
5. Chiang Y.-C., Yang C.-Y., Li C., Ho Y.-C., Lin C.-K., Tsen H.-Y. Identification of Bacillus spp., Escherichia coli, Salmonella spp., Staphylococcus spp. and Vibrio spp. with 16S ribosomal DNA-based oligonucleotide array hybridization. International Journal of Food Microbiology 2006, 107(2):131-137.
6. Clayton C., Kleanthous H., Coates P., Morgan D., Tabaqchali S. Sensitive detection of Helicobacter pylori by using polymerase chain reaction. Journal of Clinical Microbiology 1992, 30(1):192-200.
7. De Clerck E., Van Mol K., Jannes G., Rossau R., De Vos P. Design of a 5' exonuclease-based real-time PCR assay for simultaneous detection of Bacillus licheniformis, members of the B.cereus group and B.fumarioli in gelatine. Letters in Applied Microbiology 2004, 39(1):109-115.
8. Dierick K., Van Coillie E., Swiecicka I., Meyfroidt G., Devlieger H., Meulemans A., et al. Fatal family outbreak of Bacillus cereus-associated food poisoning. Journal of Clinical Microbiology 2005, 43(8):4277-4279.
9. Diez-Valcarce M., Kovač K., Cook N., Rodríguez-Lázaro D., Hernández M. Construction and analytical application of internal amplification controls (IAC) for detection of food supply chain-relevant viruses by real-time PCR-based assays. Food Analytical Methods 2011, 4(3):437-445.
10. Drobniewski F.A. Bacillus cereus and related species. Clinical Microbiology Reviews 1993, 6(4):324-338.
11. Dzieciol M., Fricker M., Wagner M., Hein I., Ehling-Schulz M. Anovel diagnostic real-time PCR assay for quantification and differentiation of emetic and non-emetic Bacillus cereus. Food Control 2013, 32(2013):176-185.
12. Ehling-Schulz M., Fricker M., Grallert H., Rieck P., Wagner M., Scherer S. Cereulide synthetase gene cluster from emetic Bacillus cereus: structure and location on a mega virulence plasmid related to Bacillus anthracis toxin plasmid pXO1. BMC Microbiology 2006, 6(1):1-11.
13. Ehling-Schulz M., Fricker M., Scherer S. Bacillus cereus, the causative agent of an emetic type of food-borne illness. Molecular Nutrition & Food Research 2004, 48(7):479-487.
14. Ehling-Schulz M., Fricker M., Scherer S. Identification of emetic toxin producing Bacillus cereus strains by a novel molecular assay. FEMS Microbiology Letters 2006, 232(2):189-195.
15. Fricker M., Messelhäußer U., Busch U., Scherer S., Ehling-Schulz M. Diagnostic real-time PCR assays for the detection of emetic Bacillus cereus strains in foods and recent food-borne outbreaks. Applied and Environmental Microbiology 2007, 73(6):1892-1898.
16. Fricker M., Reissbrodt R., Ehling-Schulz M. Evaluation of standard and new chromogenic selective plating media for isolation and identification of Bacillus cereus. International Journal of Food Microbiology 2008, 121(1):27-34.
17. Granum P.E., Lund T. Bacillus cereus and its food poisoning toxins. FEMS Microbiology Letters 2006, 157(2):223-228.
18. Hoorfar J., Cook N., Malorny B., Wagner M., De Medici D., Abdulmawjood A., et al. Making internal amplification control mandatory for diagnostic PCR. Journal of Clinical Microbiology 2003, 41(12). 5835-5835.
19. Lee D., Kim K., Kwon K., Hong K. Amultiplex PCR assay for the detection and differentiation of enterotoxin-producing and emetic toxin-producing Bacillus cereus strains. Food Science and Biotechnology 2008, 17(4):761-765.
20. Lestari S.I., Han F., Wang F., Ge B. Prevalence and antimicrobial resistance of Salmonella serovars in conventional and organic chickens from Louisiana retail stores. Journal of Food Protection 2009, 72(6):1165-1172.
21. Lim J.S., Kim M.R., Kim W., Hong K.W. Detection and differentiation of non-emetic and emetic Bacillus cereus strains in food by real-time PCR. Journal of the Korean Society for Applied Biological Chemistry 2011, 54(1):105-111.
22. Nocker A., Cheung C.Y., Camper A.K. Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cells. Journal of Microbiological Methods 2006, 67(2):310-320.
23. Nocker A., Mazza A., Masson L., Camper A.K., Brousseau R. Selective detection of live bacteria combining propidium monoazide sample treatment with microarray technology. Journal of Microbiological Methods 2009, 76(3):253-261.
24. Nocker A., Sossa-Fernandez P., Burr M.D., Camper A.K. Use of propidium monoazide for live/dead distinction in microbial ecology. Applied and Environmental Microbiology 2007, 73(16):5111-5117.
25. Nogva H.K., Dromtorp S., Nissen H., Rudi K. Ethidium monoazide for DNA-based differentiation of viable and dead bacteria by 5'-nuclease PCR. BioTechniques 2003, 34(4):804-813.
26. Park Y.B., Kim J.B., Shin S.W., Kim J.C., Cho S.H., Lee B.K., et al. Prevalence, genetic diversity, and antibiotic susceptibility of Bacillus cereus strains isolated from rice and cereals collected in Korea. Journal of Food Protection 2009, 72(3):612-617.
27. Shiota M., Saitou K., Mizumoto H., Matsusaka M., Agata N., Nakayama M., et al. Rapid detoxification of cereulide in Bacillus cereus food poisoning. Pediatrics 2010, 125(4):e951-e955.
28. Wan C., Yang Y., Xu H., Aguilar Z.P., Liu C., Lai W., et al. Development of a propidium monoazide treatment combined with loop-mediated isothermal amplification (PMA-LAMP) assay for rapid detection of viable Listeria monocytogenes. International Journal of Food Science & Technology 2012, 47(2011):2460-2467.
29. Wang L., Li Y., Mustapha A. Detection of viable Escherichia coli O157:H7 by ethidium monoazide real-time PCR. Journal of Applied Microbiology 2009, 107(5):1719-1728.
30. Wang S., Levin R.E. Discrimination of viable Vibrio vulnificus cells from dead cells in real-time PCR. Journal of Microbiological Methods 2006, 64(1):1-8.
31. Wang X., Zhu C., Xu X., Zhou G. Real-time PCR with internal amplification control for the detection of Cronobacter spp. (Enterobacter sakazakii) in food samples. Food Control 2012, 25(2012):144-149.
32. Wehrle E., Moravek M., Dietrich R., Bürk C., Didier A., Märtlbauer E. Comparison of multiplex PCR, enzyme immunoassay and cell culture methods for the detection of enterotoxinogenic Bacillus cereus. Journal of Microbiological Methods 2009, 78(3):265-270.
33. Yáñez M.A., Nocker A., Soria-Soria E., Múrtula R., Martínez L., Catalán V. Quantification of viable Legionella cells using propidium monoazide combined with quantitative PCR. Journal of Microbiological Methods 2011, 85(2):124-130.
34. Yang I., Shih D.Y.-C., Wang J.-Y., Pan A.-M. Development of rapid real-time PCR and most-probable-number real-time PCR assays to quantify enterotoxigenic strains of the species in the Bacillus cereus group. Journal of Food Protection 2007, 70(12):2774-2781.
35. Yang Y., Wan C., Xu H., Lai W., Xiong Y., Xu F., et al. Development of a multiplexed PCR assay combined with propidium monoazide treatment for rapid and accurate detection and identification of three viable Salmonella enterica serovars. Food Control 2012, 28(2012):456-462.
36. Zhu R.G., Li T.P., Jia Y.F., Song L.F. Quantitative study of viable Vibrio parahaemolyticus cells in raw seafood using propidium monoazide in combination with quantitative PCR. Journal of Microbiological Methods 2012, 90(3):262-266.