Application of propidium monoazide-qPCR to evaluate the ultrasonic inactivation of Escherichia coli O157:H7 in fresh-cut vegetable wash water

Food Microbiology

Volumn 30, Issue 1, 2012, Pages 316-320

  Elizaquivel P ^a   Sanchez G ^b   Selma, M V ^c   Aznar, R ^a,b  

^a UNIVERSITY OF VALENCIA   (Spain)

^b INSTITUTO DE AGROQUÍMICA Y TECNOLOGÍA DE ALIMENTOS CSIC   (Spain)

^c RESEARCH GROUP ON QUALITY   (Spain)

Author keywords

Enterohemorrhagic E.coli; Fresh cut vegetables; Propidium monoazide; Ultrasound

Indexed keywords

AZIDE; DRUG DERIVATIVE; PROPIDIUM IODIDE; PROPIDIUM MONOAZIDE;

ARTICLE; BACTERIAL COUNT; CELL SURVIVAL; DISINFECTION; ESCHERICHIA COLI O157; FOOD CONTAMINATION; FOOD CONTROL; FOOD HANDLING; GROWTH, DEVELOPMENT AND AGING; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MICROBIOLOGY; PRODUCT SAFETY; REAL TIME POLYMERASE CHAIN REACTION; VEGETABLE;

AZIDES; CELL SURVIVAL; COLONY COUNT, MICROBIAL; CONSUMER PRODUCT SAFETY; DISINFECTION; ESCHERICHIA COLI O157; FOOD CONTAMINATION; FOOD HANDLING; FOOD MICROBIOLOGY; PROPIDIUM; REAL-TIME POLYMERASE CHAIN REACTION; VEGETABLES;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; LACTUCA;

EID: 84856054274     PISSN: 07400020     EISSN: 10959998     Source Type: Journal    
DOI: 10.1016/j.fm.2011.10.008     Document Type: Article

References (40)

1. Ackers M.L., Mahon B.E., Leahy E., Goode B., Damrow T., Hayes P.S., et al. An outbreak of Escherichia coli O157:H7 infections associated with leaf lettuce consumption. J.Infect. Dis. 1998, 177:1588-1593.
2. APHA, American Public Health Association Standard Methods for the Examination of Water and Wastewater 1998, American Public Health Association, Washington, D.C. twentieth ed.
3. Bigelow W.D., Esty J.R. The thermal death point in relation to typical thermophylic organisms. J.Infect. Dis. 1920, 27:602.
4. Doyle M.P., Erickson M.C. Summer meeting 2007-the problems with fresh produce: an overview. J.Appl. Microbiol. 2008, 105:317-330.
5. Drakopoulou S., Terzakis S., Fountoulakis M.S., Mantzavinos D., Manios T. Ultrasound-induced inactivation of gram-negative and gram-positive bacteria in secondary treated municipal wastewater. Ultrason. Sonochem. 2009, 16:629-634.
6. Elizaquível P., Aznar R. Comparison of four commercial DNA extraction kits for PCR detection of Listeria monocytogenes, Salmonella, Escherichia coli O157:H7, and Staphylococcus aureus in fresh, minimally processed vegetables. J.Food Prot. 2008, 71:2110-2114.
7. Elizaquível P., Gabaldón J.A., Aznar R. Quantification of Salmonella spp., Listeria monocytogenes and Escherichia coli O157:H7 in non-spiked food products and evaluation of real-time PCR as a diagnostic tool in routine food analysis. Food Control 2011, 22:158-164.
8. Geeraerd A.H., Valdramidis V.P., Van Impe J.F. GInaFiT, a freeware tool to assess non-log-linear microbial survivor curves. Int. J. Food Microbiol. 2005, 102:95-105.
9. Gil M.I., Selma M.V., López-Galvez F., Allende A. Fresh-cut product sanitation and wash water disinfection: problems and solutions. Int. J. Food Microbiol. 2009, 134:37-45.
10. Grant J., Wendelboe A.M., Wendel A., Jepson B., Torres P., Smelser C., et al. Spinach-associated Escherichia coli O157:H7 outbreak, Utah and New Mexico, 2006. Emerg. Infect. Dis. 2008, 14:1633-1636.
11. Hao J., Liu H., Liu R., Dalai W., Zhao R., Chen T., Li L. Efficacy of slightly acidic electrolyzed water (Saew) for reducing microbial contamination on fresh-cut cilantro. J.Food Saf. 2011, 31:28-34.
12. Higuchi R., Fockler C., Dollinger G., Watson R. Kinetic PCR analysis: real-time monitoring of DNA amplification reactions. Biotechnol. 1993, 11:1026-1030.
13. Hulsmans A., Joris K., Lambert N., Rediers H., Declerck P., Delaedt Y., et al. Evaluation of process parameters of ultrasonic treatment of bacterial suspensions in a pilot scale water disinfection system. Ultrason. Sonochem. 2010, 17:1004-1009.
14. Jinneman K.C., Yoshitomi K.J., Weagant S.D. Multiplex real-time PCR method to identify Shiga toxin genes stx1 and stx2 and Escherichia coli O157:H7/H- serotype. Appl. Environ. Microbiol. 2003, 69:6327-6333.
15. Johnston L.M., Jaykus L.A., Moll D., Anciso J., Mora B., Moe C.L. Afield study of the microbiological quality of fresh produce of domestic and Mexican origin. Int. J. Food Microbiol. 2006, 112:83-95.
16. Josefsen M.H., Lofstrom C., Hansen T.B., Christensen L.S., Olsen J.E., Hoorfar J. Rapid quantification of viable Campylobacter bacteria on chicken carcasses, using real-time PCR and propidium monoazide treatment, as a tool for quantitative risk assessment. Appl. Environ. Microbiol. 2010, 76:5097-5104.
17. Kim H.J., Feng H., Kushad M.M., Fan X. Effects of ultrasound, irradiation, and acidic electrolyzed water on germination of alfalfa and broccoli seeds and Escherichia coli O157:H7. J.Food Saf. 2006, 17:168-173.
18. Lynch M.F., Tauxe R.V., Hedberg C.W. The growing burden of foodborne outbreaks due to contaminated fresh produce: risks and opportunities. Epidemiol. Infect. 2009, 137:307-315.
19. MacKay I.M. Real-time PCR in the microbiology laboratory. Clin. Microbiol. Infect. 2004, 10:190-212.
20. Mafart P., Couvert O., Gaillard S., Leguerinel I. On calculating sterility in thermal preservation methods: application of the Weibull frequency distribution model. Int. J. Food Microbiol. 2002, 72:107-113.
21. Mason T.J., Joyce E., Phull S.S., Lorimer J.P. Potential uses of ultrasound in the biological decontamination of water. Ultrason. Sonochem. 2003, 10:319-323.
22. McKillip J.L., Drake M.A. Real-time nucleic acid-based detection methods for pathogenic bacteria in food. J.Food Prot. 2004, 67:823-832.
23. Nocker A., Camper A.K. Novel approaches toward preferential detection of viable cells using nucleic acid amplification techniques. FEMS Microbiol. Lett. 2009, 291:137-142.
24. Nocker A., Cheung C.Y., Camper A.K. Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cells. J.Microbiol. Methods 2006, 67:310-320.
25. Nocker A., Sossa K.E., Camper A.K. Molecular monitoring of disinfection efficacy using propidium monoazide in combination with quantitative PCR. J.Microbiol. Methods 2007, 70:252-260.
26. Nogva H.K., Dromtorp S.M., Nissen H., Rudi K. Ethidium monoazide for DNA-based differenciation of viable and dead bacteria by 5'-nuclease PCR. Biotech. 2003, 34:804-813.
27. Pan Y., Breidt F. Enumeration of viable Listeria monocytogenes celles by real-time PCR with propidium monoazide and ethidium monoazide in the presence of dead cells. Appl. Environ. Microbiol. 2007, 73:8028-8031.
28. Perelle S., Dilasser F., Grout J., Fach P. Screening food raw materials for the presence of the world's most frequent clinical cases of shiga toxin-encoding Escherichia coli O26, O103, O111, O145 and O157. Int. J. Food Microbiol. 2007, 113:284-288.
29. Ratkowsky D.A. Some examples of, and problems with, the use of nonlinear logistic regression in predictive food microbiology. Int J. Food Microbiol. 2002, 73:119-125.
30. Sagong H.G., Lee S.Y., Chang P.S., Heu S., Ryu S., Choi Y.J., et al. Combined effect of ultrasound and organic acids to reduce Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes on organic fresh lettuce. Int J. Food Microbiol. 2011, 145:287-292.
31. Selma M.V., Allende A., López-Gálvez F., Conesa M.A., Gil M.I. Disinfection potential of ozone, ultraviolet-C and their combination in wash water for the fresh-cut vegetable industry. Food Microbiol. 2008, 25:809-814.
32. Selma M.V., Allende A., López-Gálvez F., Conesa M.A., Gil M.I. Heterogeneous photocatalytic disinfection of wash waters from the fresh-cut vegetable industry. J.Food Prot. 2008, 71:286-292.
33. Seymour I.J., Burfoot D., Smith R.L., Cox L.A., Lockwood A. Ultrasound decontamination of minimally processed fruits and vegetables. Int. J. Food Sci. Technol. 2002, 37:547-557.
34. Soderstrom A., Osterberg P., Lindqvist A., Jonsson B., Lindberg A., Blide U.S., et al. Alarge Escherichia coli O157 outbreak in Sweden associated with locally produced lettuce. Foodborne Path. Dis. 2008, 5:339-349.
35. Uhlich G.A., Sinclair J.R., Warren N.G., Chmielecki W.A., Fratamico P. Characterization of Shiga toxin-producing Escherichia coli isolates associated with two multistate food-borne outbreaks that occurred in 2006. Appl. Environ. Microbiol. 2008, 74:1268-1272.
36. Vandekinderen I., Van Camp J., Devlieghere F., Ragaert P., Veramme K., Bernaert N., Denon Q., De Meulenaer B. Evaluation of the use of decontamination agents during fresh-cut leek processing and quantification of their effect on its total quality by means of a multidisciplinary approach. Innov. Food Sci. Emerg. Tech. 2009, 10:363-373.
37. Wendel A.M., Johnson D.H., Sharapov U., Grant J., Archer J.R., Monson T., et al. Multistate outbreak of Escherichia coli O157:H7 infection associated with consumption of packaged spinach, August-September 2006: the Wisconsin investigation. Clin. Infect. Dis. 2009, 48:1079-1086.
38. Yoshitomi K.J., Jinneman K.C., Weagant S.D. Detection of Shiga toxin genes stx1, stx2, and the+93 uidA mutation of E.coli O157:H7/H- using SYBR Green I in a real-time multiplex PCR. Mol. Cell. Probes 2006, 20:31-41.
39. Zhang S., Yu X. PMA in combination with quantitative PCR for the detection of inactivation efficacy by ultrasonic sonication. Fresenius Environ. Bull. 2010, 19:940-944.
40. Zhou B., Feng H., Luo Y. Ultrasound enhanced sanitizer efficacy in reduction of Escherichia coli O157:H7 population on spinach leaves. J.Food Sci. 2009, 74:M308-M313.