Development of an immunomagnetic separation-propidium monoazide-polymerase chain reaction assay with internal amplification control for rapid and sensitive detection of viable Escherichia coli O157: H7 in milk

International Dairy Journal

Volumn 34, Issue 2, 2014, Pages 280-286

  Wang, Lijun ^a   Li, Ping ^a   Yang, Youjun ^a   Xu, Hong ^b   Aguilar, Zoraida P ^b   Xu, Hengyi ^a   Yang, Lin ^a   Xu, Feng ^a   Lai, Weihua ^a   Xiong, Yonghua ^a   Wei, Hua ^a  

^a NANCHANG UNIVERSITY   (China)

^b OCEAN NANOTECH LLC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CONVENTIONAL METHODS; DETECTION LIMITS; ESCHERICHIA COLI O157; ESCHERICHIA COLI O157:H7; FALSE NEGATIVES; IMMUNOMAGNETIC SEPARATION; INTERNAL AMPLIFICATION; SENSITIVE DETECTION;

ASSAYS; ESCHERICHIA COLI;

POLYMERASE CHAIN REACTION;

ESCHERICHIA COLI;

EID: 84887821582     PISSN: 09586946     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.idairyj.2013.07.006     Document Type: Article

References (42)

1. Aguilar Z.P. Rapid small volume microelectrochemical DNA-hybridization detection of plasmodium falciparum. Analytical Chemistry 2006, 78:1122-1129.
2. Aguilar Z.P., Fritsch I. DNA-hybridization sensor for C.parvum hsp70 mRNA gene. Analytical Chemistry 2003, 75:3890-3897.
3. Al-Soud W.A., Rådström P. Purification and characterization of PCR-inhibitory components in blood cells. Journal of Clinical Microbiology 2001, 39:485-493.
4. Bai Y., Huang W.C., Yang S.T. Enzyme-linked immunosorbent assay of Escherichia coli O157:H7 in surface enhanced poly (methyl methacrylate) microchannels. Biotechnology and Bioengineering 2007, 98:328-339.
5. Bickley J., Short J.K., McDowell D.G., Parkes H.C. Polymerase chain reaction (PCR) detection of Listeria monocytogenes in diluted milk and reversal of PCR inhibition caused by calcium ions. Letters in Applied Microbiology 1996, 22:153-158.
6. Bioterrorism Agents/Diseases CDC-centers for disease control and prevention 2004, [online] Available. http://www.bt.cdc.gov/agent/agentlist-category.asp.
7. CDC Summary of outbreaks of Escherichia coli O157 and other Shiga toxin-producing E.coli reported to the CDC in 1999 1999, Center for Disease Control, Atlanta, GA, USA.
8. CDC Outbreaks caused by Shiga toxin-producing Escherichia coli O157. Summary of 2000 surveillance data 2001, Center for Disease Control, Atlanta, GA, USA.
9. Chen J., Tang J., Liu J., Cai Z., Bai X. Development and evaluation of a multiplex PCR for simultaneous detection of five foodborne pathogens. Journal of Applied Microbiology 2012, 112:823-830.
10. Chiang Y.C., Yang C.Y., Li C., Ho Y.C., Lin C.K., Tsen H.Y. Identification of Bacillus spp., Escherichia coli, Salmonella spp., Staphylococcus spp. and Vibrio spp. with 16S ribosomal DNA-based oligonucleotide array hybridization. International Journal of Food Microbiology 2006, 107:131-137.
11. Dinu L.D., Bach S. Induction of viable but nonculturable Escherichia coli O157:H7 in the phyllosphere of lettuce: a food safety risk factor. Applied and Environmental Microbiology 2011, 77:8295-8302.
12. Elizaquível P., Sánchez G., Aznar R. Quantitative detection of viable foodborne E.coli O157:H7, Listeria monocytogenes and Salmonella in fresh-cut vegetables combining propidium monoazide and real-time PCR. Food Control 2011, 25:704-708.
13. Farrokh C., Jordan K., Auvray F., Glass K., Oppegaard H., Raynaud S., et al. Review of Shiga-toxin-producing Escherichia coli (STEC) and their significance in dairy production. International Journal of Food Microbiology 2013, 162:190-212.
14. Fedio W.M., Jinneman K.C., Yoshitomi K.J., Zapata R., Wendakoon C.N., Browning P., et al. Detection of E.coli O157:H7 in raw ground beef by Pathatrix™ immunomagnetic-separation, real-time PCR and cultural methods. International Journal of Food Microbiology 2011, 148:87-92.
15. Feng P., Weagant S.D., Jinneman K. Diarrheagenic Escherichia coli. Bacteriological analytical manual 2011, (chap. 4A). http://www.fda.gov/Food/FoodScienceResearch/LaboratoryMethods/ucm070080.htm.
16. Fricker M., Messelhäußer U., Busch U., Scherer S., Ehling-Schulz M. Diagnostic real-time PCR assays for the detection of emetic Bacillus cereus strains in foods and recent food-borne outbreaks. Applied and Environmental Microbiology 2007, 73:1892-1898.
17. Fu Z., Rogelj S., Kieft T.L. Rapid detection of Escherichia coli O157:H7 by immunomagnetic separation and real-time PCR. International Journal of Food Microbiology 2005, 99:47-57.
18. Gillespie I.A., O'Brien S.J., Adak G.K., Cheasty T., Willshaw G. Foodborne general outbreaks of Shiga toxin-producing Escherichia coli O157 in England and Wales 1992-2002: where are the risks?. Epidemiology and Infection 2005, 133:803-808.
19. Hoorfar J., Wolffs P., Rådström P. Diagnostic PCR: validation and sample preparation are two sides of the same coin. APMIS 2008, 112:808-814.
20. Italia J.T., Rovira H.G., Masangkay J.S., Yoshikawa Y., Perez M.T.M., Reyes A.W.B. Izdvajanje bakterije Escherichia coli O157:H7 i njezin dokaz lančanom reakcijom polimerazom u crijevima filipinskih šišmiša. Veterinarski arhiv 2012, 82:283-294.
21. Kim H.J., Cho J.C. Simple and rapid detection of Listeria monocytogenes in fruit juice by real-time PCR without enrichment culture. Food Control 2010, 21:1419-1423.
22. Lantz P.G., Matsson M., Wadström T., Rådström P. Removal of PCR inhibitors from human faecal samples through the use of an aqueous two-phase system for sample preparation prior to PCR. Journal of Microbiological Methods 1997, 28:159-167.
23. Liu D. Molecular detection of foodborne pathogens 2009, CRC Press, Boca Raton, FL, USA.
24. Liu Y., Wang C., Tyrrell G., Hrudey S.E., Li X.F. Induction of Escherichia coli O157:H7 into the viable but non-culturable state by chloraminated water and river water, and subsequent resuscitation. Environmental Microbiology Reports 2009, 1:155-161.
25. Liu Y.J., Yao D.J., Chang H.Y., Liu C.M., Chen C. Magnetic bead-based DNA detection with multi-layers quantum dots labeling for rapid detection of Escherichia coli O157:H7. Biosensors and Bioelectronics 2008, 24:558-565.
26. Lovdal T., Hovda M.B., Bjorkblom B., Moller S.G. Propidium monoazide combined with real-time quantitative PCR underestimates heat-killed Listeria innocua. Journal of Microbiological Methods 2011, 85:164-169.
27. Martinon A., Cronin U.P., Quealy J., Stapleton A., Wilkinson M.G. Swab sample preparation and viable real-time PCR methodologies for the recovery of Escherichia coli, Staphylococcus aureus or Listeria monocytogenes from artificially contaminated food processing surfaces. Food Control 2012, 24:86-94.
28. Nocker A., Cheung C.Y., Camper A.K. Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cells. Journal of Microbiological Methods 2006, 67:310-320.
29. Oliver J.D. Viable but nonculturable bacteria in food environments. Foodborne pathogens: Microbiology and molecular Biology 2005, Caister Academic Press, Norfolk, UK, (chap. 6). P.M. Fratamico, A.K. Bhunia, J.L. Smith (Eds.).
30. Pankhurst Q.A., Connolly J., Jones S.K., Dobson J. Applications of magnetic nanoparticles in biomedicine. Journal of Physics D: Applied Physics 2003, 36:R167-R181.
31. Phillips C.A. The epidemiology, detection and control of Escherichia coli O157. Journal of the Science of Food and Agriculture 1999, 79:1367-1381.
32. Reilly A. Prevention and control of enterohaemorrhagic Escherichia coli (EHEC) infections: memorandum from a WHO meeting. WHO consultation on prevention and control of enterohaemorrhagic Escherichia coli (EHEC) infections. Bulletin of the World Health Organization 1998, 76:245-255.
33. Sarimehmetoglu B., Aksoy M.H., Ayaz N.D., Ayaz Y., Kuplulu O., Kaplan Y.Z. Detection of Escherichia coli O157:H7 in ground beef using immunomagnetic separation and multiplex PCR. Food Control 2009, 20:357-361.
34. Tram L.L.T., Cao C., Høgberg J., Wolff A., Bang D.D. Isolation and detection of Campylobacter jejuni from chicken fecal samples by immunomagnetic separation-PCR. Food Control 2011, 24:23-28.
35. Voitoux E., Lafarge V., Collette C., Lombard B. Applicability of the draft standard method for the detection of Escherichia coli O157 in dairy products. International Journal of Food Microbiology 2002, 77:213-221.
36. Wang L., Li Y., Mustapha A. Detection of viable Escherichia coli O157:H7 by ethidium monoazide real-time PCR. Journal of Applied Microbiology 2009, 107:1719-1728.
37. Xu H., Aguilar Z.P., Yang L., Kuang M., Duan H., Xiong Y., et al. Antibody conjugated magnetic iron oxide nanoparticles for cancer cell separation in fresh whole blood. Biomaterials 2011, 32:9758-9765.
38. Yang Y.J., Wan C.X., Xu H.Y., Lai W.H., Xiong Y.H., Xu F., et al. Development of a multiplexed PCR assay combined with propidium monoazide treatment for rapid and accurate detection and identification of three viable Salmonella enteric serovars. Food Control 2012, 28:456-462.
39. Yang Z.Y., Shim W.B., Kim K.Y., Chung D.H. Rapid detection of Enterotoxigenic Clostridium perfringens in meat samples using immunomagnetic separation polymerase chain reaction (IMS-PCR). Journal of Agricultural and Food Chemistry 2010, 58:7135-7140.
40. Yoshitomi K.J., Jinneman K.C., Zapata R., Weagant S.D., Fedio W.M. Detection and isolation of low levels of E.coli O157:H7 in cilantro by real-time PCR, immunomagnetic separation, and cultural methods with and without an acid treatment. Journal of Food Science 2012, 77:M481-M489.
41. Zhao X.H., He X.W., Li W.M., Liu Y., Yang L.S., Wang J.H. Development and evaluation of colloidal gold immunochromatographic strip for detection of Escherichia coli O157. African Journal of Microbiology Research 2010, 4:663-670.
42. Zhao X.H., Li Y.M., Wang L., You L.J., Xu Z.B., Li L., et al. Development and application of a loop-mediated isothermal amplification method on rapid detection Escherichia coli O157 strains from food samples. Molecular Biology Reports 2010, 37:2183-2188.