MyD88 or TRAM knockdown regulates interleukin (IL)-6, IL-8, and CXCL12 mRNA expression in human gingival and periodontal ligament fibroblasts

Journal of Periodontology

Volumn 84, Issue 9, 2013, Pages 1353-1360

  Morandini, Ana Carolina ^a   Souza, Pedro Paulo Chaves ^b   Ramos Jr , Erivan Schnaider ^c   Costa, Carlos Alberto Souza ^b   Santos, Carlos Ferreira ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b SÃO PAULO STATE UNIVERSITY UNESP   (Brazil)

^c FEDERAL UNIVERSITY OF RIO DE JANEIRO   (Brazil)

Author keywords

Cytokines; Fibroblasts; Lipopolysaccharides; Myeloid differentiation factor 88; Porphyromonas gingivalis

Indexed keywords

CXCL12 PROTEIN, HUMAN; IL6 PROTEIN, HUMAN; IL8 PROTEIN, HUMAN; INTERLEUKIN 6; INTERLEUKIN 8; LIGAND; LIPOPEPTIDE; LIPOPOLYSACCHARIDE; MESSENGER RNA; MYD88 PROTEIN, HUMAN; MYELOID DIFFERENTIATION FACTOR 88; PAM(3)CSK(4) PEPTIDE; PAM2CSK4 LIPOPEPTIDE; SIGNAL TRANSDUCING ADAPTOR PROTEIN; SMALL INTERFERING RNA; STROMAL CELL DERIVED FACTOR 1; TICAM2 PROTEIN, HUMAN; TOLL LIKE RECEPTOR 2;

ADOLESCENT; ADULT; ARTICLE; CELL SURVIVAL; CULTURE TECHNIQUE; CYTOLOGY; DRUG EFFECT; DRUG POTENTIATION; FEMALE; FIBROBLAST; GENE EXPRESSION REGULATION; GENE SILENCING; GENETICS; GINGIVA; HUMAN; IMMUNOLOGY; MALE; METHODOLOGY; PERIODONTAL LIGAMENT; PHYSIOLOGY; PORPHYROMONAS GINGIVALIS; UPREGULATION; YOUNG ADULT;

ADAPTOR PROTEINS, SIGNAL TRANSDUCING; ADOLESCENT; ADULT; CELL CULTURE TECHNIQUES; CELL SURVIVAL; CHEMOKINE CXCL12; FEMALE; FIBROBLASTS; GENE EXPRESSION REGULATION; GENE KNOCKDOWN TECHNIQUES; GINGIVA; HUMANS; INTERLEUKIN-6; INTERLEUKIN-8; LIGANDS; LIPOPEPTIDES; LIPOPOLYSACCHARIDES; MALE; MYELOID DIFFERENTIATION FACTOR 88; PERIODONTAL LIGAMENT; PORPHYROMONAS GINGIVALIS; RNA, MESSENGER; RNA, SMALL INTERFERING; TOLL-LIKE RECEPTOR 2; UP-REGULATION; YOUNG ADULT;

EID: 84883356104     PISSN: 00223492     EISSN: None     Source Type: Journal    
DOI: 10.1902/jop.2012.120496     Document Type: Article

References (43)

1. Akira S, Uematsu S, Takeuchi O. Pathogen recognition and innate immunity. Cell 2006;124:783-801.
2. Darveau RP, Hajishengallis G, Curtis MA. Porphyromonas gingivalis as a potential community activist for disease. J Dent Res 2012;91:816-820.
3. Hajishengallis G, Liang S, Payne MA, et al. Lowabundance biofilm species orchestrates inflammatory periodontal disease through the commensal microbiota and complement. Cell Host Microbe 2011; 10:497-506.
4. Wang PL, Azuma Y, Shinohara M, Ohura K. Toll-like receptor 4-mediated signal pathway induced by Porphyromonas gingivalis lipopolysaccharide in human gingival fibroblasts. Biochem Biophys Res Commun 2000;273:1161-1167.
5. O'Neill LA, Bowie AG. The family of five: TIR-domaincontaining adaptors in Toll-like receptor signalling. Nat Rev Immunol 2007;7:353-364.
6. Yamaji Y, Kubota T, Sasaguri K, et al. Inflammatory cytokine gene expression in human periodontal ligament fibroblasts stimulated with bacterial lipopolysaccharides. Infect Immun 1995;63:3576-3581.
7. Wang PL, Oido-Mori M, Fujii T, et al. Heterogeneous expression of Toll-like receptor 4 and downregulation of Toll-like receptor 4 expression on human gingival fibroblasts by Porphyromonas gingivalis lipopolysaccharide. Biochem Biophys Res Commun 2001;288: 863-867.
8. Uehara A, Takada H. Functional TLRs and NODs in human gingival fibroblasts. J Dent Res 2007;86:249-254.
9. Tang L, Zhou XD, Wang Q, et al. Expression of TRAF6 and pro-inflammatory cytokines through activation of TLR2, TLR4, NOD1, and NOD2 in human periodontal ligament fibroblasts. Arch Oral Biol 2011;56:1064-1072.
10. Morandini AC, Chaves Souza PP, Ramos-Junior ES, et al. Toll-like receptor 2 knockdown modulates interleukin (IL)-6 and IL-8 but not stromal derived factor-1 (SDF-1/CXCL12) in human periodontal ligament and gingival fibroblasts. J Periodontol 2013;84: 535-544.
11. Reife RA, Coats SR, Al-Qutub M, et al. Porphyromonas gingivalis lipopolysaccharide lipid A heterogeneity: Differential activities of tetra-and penta-acylated lipid A structures on E-selectin expression and TLR4 recognition. Cell Microbiol 2006;8:857-868.
12. Coats SR, Jones JW, Do CT, et al. Human Toll-like receptor 4 responses to P. gingivalis are regulated by lipid A 1-and 49-phosphatase activities. Cell Microbiol 2009;11:1587-1599.
13. Burns E, Eliyahu T, Uematsu S, Akira S, Nussbaum G. TLR2-dependent inflammatory response to Porphyromonas gingivalis is MyD88 independent, whereas MyD88 is required to clear infection. J Immunol 2010;184:1455-1462.
14. Ogawa T, Asai Y, Hashimoto M, et al. Cell activation by Porphyromonas gingivalis lipid A molecule through Toll-like receptor 4-and myeloid differentiation factor 88-dependent signaling pathway. Int Immunol 2002; 14:1325-1332.
15. Darveau RP, Pham TT, Lemley K, et al. Porphyromonas gingivalis lipopolysaccharide contains multiple lipid A species that functionally interact with both tolllike receptors 2 and 4. Infect Immun 2004;72:5041-5051.
16. Lapidot T, Dar A, Kollet O. How do stem cells find their way home? Blood 2005;106:1901-1910.
17. Lau TT, Wang DA. Stromal cell-derived factor-1 (SDF-1): Homing factor for engineered regenerative medicine. Expert Opin Biol Ther 2011;11:189-197.
18. Papanicolaou DA, Wilder RL, Manolagas SC, Chrousos GP. The pathophysiologic roles of interleukin-6 in human disease. Ann Intern Med 1998;128:127-137.
19. Morandini AC, Sipert CR, Gasparoto TH, et al. Differential production of macrophage inflammatory protein-1alpha, stromal-derived factor-1, and IL-6 by human cultured periodontal ligament and gingival fibroblasts challenged with lipopolysaccharide from P. gingivalis. J Periodontol 2010;81:310-317.
20. Baggiolini M, Walz A, Kunkel SL. Neutrophil-activating peptide-1/interleukin 8, a novel cytokine that activates neutrophils. J Clin Invest 1989;84:1045-1049.
21. Morandini AC, Sipert CR, Ramos-Junior ES, Brozoski DT, Santos CF. Periodontal ligament and gingival fibroblasts participate in the production of TGF-b, interleukin (IL)-8 and IL-10. Braz Oral Res 2011;25: 157-162.
22. Hatakeyama J, Tamai R, Sugiyama A, Akashi S, Sugawara S, Takada H. Contrasting responses of human gingival and periodontal ligament fibroblasts to bacterial cell-surface components through the CD14/Toll-like receptor system. Oral Microbiol Immunol 2003;18:14-23.
23. Peled A, Grabovsky V, Habler L, et al. The chemokine SDF-1 stimulates integrin-mediated arrest of CD34(+) cells on vascular endothelium under shear flow. J Clin Invest 1999;104:1199-1211.
24. Scimone ML, Felbinger TW, Mazo IB, Stein JV, Von Andrian UH, Weninger W. CXCL12 mediates CCR7-independent homing of central memory cells, but not naive T cells, in peripheral lymph nodes. J Exp Med 2004;199:1113-1120.
25. Ceradini DJ, Kulkarni AR, Callaghan MJ, et al. Progenitor cell trafficking is regulated by hypoxic gradients through HIF-1 induction of SDF-1. Nat Med 2004;10: 858-864.
26. Havens AM, Chiu E, Taba M, et al. Stromal-derived factor-1alpha (CXCL12) levels increase in periodontal disease. J Periodontol 2008;79:845-853.
27. Asakawa T, Chosa N, Yoshimura Y, et al. Fibroblast growth factor 2 inhibits the expression of stromal cellderived factor 1a in periodontal ligament cells derived from human permanent teeth in vitro. Int J Mol Med 2012;29:569-573.
28. Tamai R, Sakuta T, Matsushita K, et al. Human gingival CD14(+) fibroblasts primed with gamma interferon increase production of interleukin-8 in response to lipopolysaccharide through up-regulation of membrane CD14 and MyD88 mRNA expression. Infect Immun 2002;70:1272-1278.
29. Zhu L, Wu Y, Wei H, et al. Up-regulation of IL-23 p19 expression in human periodontal ligament fibroblasts by IL-1b via concurrent activation of the NF-kB and MAPKs/AP-1 pathways. Cytokine 2012;60:171-178.
30. Pablos JL, Santiago B, Galindo M, et al. Synoviocytederived CXCL12 is displayed on endothelium and induces angiogenesis in rheumatoid arthritis. J Immunol 2003;170:2147-2152.
31. Sipert CR, Moraes IG, Bernardinelli N, et al. Heat-killed Enterococcus faecalis alters nitric oxide and CXCL12 production but not CXCL8 and CCL3 production by cultured human dental pulp fibroblasts. J Endod 2010; 36:91-94.
32. Brown J, Wang H, Hajishengallis GN, Martin M. TLRsignaling networks: An integration of adaptor molecules, kinases, and cross-talk. J Dent Res 2011;90: 417-427.
33. Choi YJ, Im E, Chung HK, Pothoulakis C, Rhee SH. TRIF mediates Toll-like receptor 5-induced signaling in intestinal epithelial cells. J Biol Chem 2010;285: 37570-37578.
34. Sacre SM, Lundberg AM, Andreakos E, Taylor C, Feldmann M, Foxwell BM. Selective use of TRAM in lipopolysaccharide (LPS) and lipoteichoic acid (LTA) induced NF-kappaB activation and cytokine production in primary human cells: TRAM is an adaptor for LPS and LTA signaling. J Immunol 2007;178:2148-2154.
35. Ohnishi H, Tochio H, Kato Z, et al. TRAM is involved in IL-18 signaling and functions as a sorting adaptor for MyD88. PLoS One 2012;7:e38423.
36. Bin LH, Xu LG, Shu HB. TIRP, a novel Toll/interleukin-1 receptor (TIR) domain-containing adapter protein involved in TIR signaling. J Biol Chem 2003;278:24526-24532.
37. Dunne A, Ejdeback M, Ludidi PL, O'Neill LA, Gay NJ. Structural complementarity of Toll/interleukin-1 receptor domains in Toll-like receptors and the adaptors Mal and MyD88. J Biol Chem 2003;278:41443-41451.
38. Oshiumi H, Sasai M, Shida K, Fujita T, Matsumoto M, Seya T. TIR-containing adapter molecule (TICAM)-2, a bridging adapter recruiting to toll-like receptor 4 TICAM-1 that induces interferon-beta. J Biol Chem 2003;278:49751-49762.
39. Sheedy FJ, O'Neill LA. The Troll in Toll: Mal and Tram as bridges for TLR2 and TLR4 signaling. J Leukoc Biol 2007;82:196-203.
40. Delano MJ, Kelly-Scumpia KM, Thayer TC, et al. Neutrophil mobilization from the bone marrow during polymicrobial sepsis is dependent on CXCL12 signaling. J Immunol 2011;187:911-918.
41. Xing Q, de Vos P, Faas MM, Ye Q, Ren Y. LPS promotes pre-osteoclast activity by up-regulating CXCR4 via TLR-4. J Dent Res 2011;90:157-162.
42. Bello-Irizarry SN, Wang J, Olsen K, Gigliotti F, Wright TW. The alveolar epithelial cell chemokine response to pneumocystis requires the adaptor molecule MyD88 and the interleukin-1 receptor but not Toll-like receptor 2 or 4. Infect Immun 2012;80:3912-3920.
43. Medzhitov R, Preston-Hurlburt P, Kopp E, et al. MyD88 is an adaptor protein in the hToll/IL-1 receptor family signaling pathways. Mol Cell 1998;2:253-258.