Optimization of pullulanase production in Escherichia coli by regulation of process conditions and supplement with natural osmolytes

Bioresource Technology

Volumn 146, Issue , 2013, Pages 379-385

  Duan, Xuguo ^a   Chen, Jian ^a   Wu, Jing ^a  

^a JIANGNAN UNIVERSITY   (China)

Author keywords

Betaine; Escherichia coli; Pullulanase; Soluble expression

Indexed keywords

ESCHERICHIA COLI;

EFFECTS OF TEMPERATURE; FEEDING STRATEGIES; INDUSTRIAL PRODUCTION; PROCESS CONDITION; PULLULANASE; RECOMBINANT ESCHERICHIA COLI; SEMISYNTHETIC MEDIUM; SOLUBLE EXPRESSION;

BETAINES;

BETAINE; GLUTAMIC ACID; ISOPROPYL THIOGALACTOSIDE; PROLINE; PULLULANASE;

CONCENTRATION (COMPOSITION); ENZYME ACTIVITY; FEEDING BEHAVIOR; GROWTH RATE; MICROBIAL COMMUNITY; OPTIMIZATION; OSMOSIS;

ARTICLE; BACTERIAL CELL; BACTERIUM CULTURE; CELL CULTURE; CELL GROWTH; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ENZYME ANALYSIS; ENZYME SYNTHESIS; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN EXPRESSION; TEMPERATURE;

ESCHERICHIA COLI;

BETAINE; ESCHERICHIA COLI; PULLULANASE; SOLUBLE EXPRESSION;

BETAINE; BIOMASS; BIOREACTORS; CULTURE MEDIA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYMES; ESCHERICHIA COLI; GLUTAMIC ACID; GLYCEROL; GLYCOSIDE HYDROLASES; INDUSTRIAL MICROBIOLOGY; ISOPROPYL THIOGALACTOSIDE; PROLINE; SOLUBILITY; TEMPERATURE; TIME FACTORS;

EID: 84882693673     PISSN: 09608524     EISSN: 18732976     Source Type: Journal    
DOI: 10.1016/j.biortech.2013.07.074     Document Type: Article

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