Cloning, expression, characterization, and biocatalytic investigation of a novel Bacilli thermostable type i pullulanase from Bacillus sp. CICIM 263

Journal of Agricultural and Food Chemistry

Volumn 60, Issue 44, 2012, Pages 11164-11172

  Li, Youran ^a   Zhang, Liang ^a   Niu, Dandan ^a   Wang, Zhengxiang ^a   Shi, Guiyang ^a  

^a JIANGNAN UNIVERSITY   (China)

Author keywords

Bacillus sp.; gene cloning and expression; thermostable type I pullulanase

Indexed keywords

BACILLUS SP; BACILLUS SUBTILIS; BASE PAIRS; BIO-ETHANOL PRODUCTION; FERMENTABLE SUGARS; GENE CLONING AND EXPRESSIONS; GENOMIC DNA; HYDROLYTIC ACTIVITIES; MALTOTRIOSE; OPEN READING FRAME; OPTIMAL ACTIVITY; PULLULANASE; PULLULANS;

AMINO ACIDS; BACTERIOLOGY; ENZYMES; ESCHERICHIA COLI; GENETIC ENGINEERING; INDUSTRIAL APPLICATIONS;

GENE ENCODING;

GLYCOSIDASE; PRIMER DNA; PULLULANASE; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; BACILLUS; BIOCATALYSIS; ELECTROSPRAY MASS SPECTROMETRY; ENZYME STABILITY; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; LIQUID CHROMATOGRAPHY; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; TANDEM MASS SPECTROMETRY;

AMINO ACID SEQUENCE; BACILLUS; BASE SEQUENCE; BIOCATALYSIS; CHROMATOGRAPHY, LIQUID; CLONING, MOLECULAR; DNA PRIMERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME STABILITY; GLYCOSIDE HYDROLASES; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TANDEM MASS SPECTROMETRY;

BACILLI (CLASS); BACILLUS (BACTERIUM); BACILLUS SP.; BACILLUS SUBTILIS; ESCHERICHIA COLI;

EID: 84868599824     PISSN: 00218561     EISSN: 15205118     Source Type: Journal    
DOI: 10.1021/jf303109u     Document Type: Article

References (47)

1. Kuriki, T.; Park, J.-H.; Okada, S.; Imanaka, T. Purification and characterization of thermostable pullulanase from Bacillus stearothermophilus and molecular cloning and expression of the gene in Bacillus subtilis Appl. Environ. Microbiol. 1988, 54, 2881-2883
2. Henrissat, B.; Bairoch, A. New families in the classification of glycosyl hydrolases based on amino acid sequence similarities Biochem. J. 1993, 293 (Pt 3) 781-788
3. Spreinat, A.; Antranikian, G. Purification and properties of a thermostable pullulanase from Clostridium thermosulfurogenes EM1 which hydrolyses both α-1,6 and α-1,4-glycosidic linkages Appl. Microbiol. Biotechnol. 1990, 33, 511-518
4. Saha, B. C.; Zeikus, J. G. Novel highly thermostable pullulanase from thermophiles Trends Biotechnol. 1989, 7, 234-239
5. Kim, C.-H.; Choi, H.-I.; Lee, D.-S. Pullulanases of alkaline and broad pH range from a newly isolated alkalophilic Bacillus sp. S-1 and a Micrococcus sp. Y-1 J. Ind. Microbiol. Biotechnol. 1993, 12, 48-57
6. Sakano, Y.; Higuchi, M.; Kobayashi, T. Pullulan 4-glucanohydrolase from Aspergillus niger Arch. Biochem. Biophys. 1972, 153, 180-187
7. Bertoldo, C.; Duffner, F.; Jorgensen, P. L.; Antranikian, G. Pullulanase Type I from Fervidobacterium pennavorans Ven5: Cloning, sequencing, and expression of the gene and biochemical characterization of the recombinant enzyme Appl. Environ. Microbiol. 1999, 65, 2084-2091
8. Jensen, K. K.; Hulett, F. M. Protoplast transformation of Bacillus licheniformis MC14 J. Gen. Microbiol. 1989, 135, 2283-2287
9. Shuichi, K.; Naokazu, N.; Shin-Ichiro, T.; Daisaburo, F.; Yoshiyuki, S. Purification and properties of Bacillus acidopullulyticus Pullulanase (biological chemistry) Agric. Biol. Chem. 1988, 52, 2293-2298
10. Shobha, M. S.; Tharanathan, R. N. Nonspecific activity of Bacillus acidopullulyticus pullulanase on debranching of guar galactomannan J. Agric. Food Chem. 2008, 56, 10858-10864
11. Nair, S. U.; Singhal, R. S.; Kamat, M. Y. Induction of pullulanase production in Bacillus cereus FDTA-13 Bioresour. Technol. 2007, 98, 856-859
12. Bertoldo, C.; Armbrecht, M.; Becker, F.; Schäfer, T.; Antranikian, G.; Liebl, W. Cloning, sequencing, and characterization of a heat- and alkali-stable Type I pullulanase from Anaerobranca gottschalkii Appl. Environ. Microbiol. 2004, 70, 3407-3416
13. Kang, J.; Park, K.-M.; Choi, K.-H.; Park, C.-S.; Kim, G.-E.; Kim, D.; Cha, J. Molecular cloning and biochemical characterization of a heat-stable type I pullulanase from Thermotoga neapolitana Enzyme Microb. Technol. 2011, 48, 260-266
14. Spizizen, J. Transformation of biochemically deficient strains of Bacillus subtilis by deoxyribonucleate Proc. Natl. Acad. Sci. U.S.A. 1958, 44, 1072-1078
15. Aiba, S.; Kitai, K.; Imanaka, T. Cloning and expression of thermostable α-amylase gene from Bacillus stearothermophilus in Bacillus stearothermophilus and Bacillus subtilis Appl. Environ. Microbiol. 1983, 46, 1059-1065
16. Paul, N, H. Purification of His-Tag fusion proteins from Escherichia coli Trends Biochem. Sci. 1995, 20, 285-286
17. Rüdiger, A.; Jorgensen, P. L.; Antranikian, G. Isolation and characterization of a heat-stable pullulanase from the hyperthermophilic archaeon Pyrococcus woesei after cloning and expression of its gene in Escherichia coli Appl. Environ. Microbiol. 1995, 61, 567-575
18. Jeon, J. H.; Kim, S.-J.; Lee, H. S.; Cha, S.-S.; Lee, J. H.; Yoon, S.-H.; Koo, B.-S.; Lee, C.-M.; Choi, S. H.; Lee, S. H.; Kang, S. G.; Lee, J.-H. Novel metagenome-derived carboxylesterase that hydrolyzes β-lactam antibiotics Appl. Environ. Microbiol. 2011, 77, 7830-7836
19. Collins, F. S.; Weissman, S. M. Directional cloning of DNA fragments at a large distance from an initial probe: A circularization method Proc. Natl. Acad. Sci. U.S.A. 1984, 81, 6812-6816
20. Niu, D. D.; Wang, Z. X. Development of a pair of bifunctional expression vectors for Escherichia coli and Bacillus licheniformis J. Ind. Microbiol. Biotechnol. 2007, 34, 357-362
21. Gruber, A. R.; Lorenz, R.; Bernhart, S. H.; Neuböck, R.; Hofacker, I. L. The Vienna RNA Websuite Nucleic Acids Res. 2008, 36, W70-W74
22. Marchler-Bauer, A.; Lu, S.; Anderson, J. B.; Chitsaz, F.; Derbyshire, M. K.; DeWeese-Scott, C.; Fong, J. H.; Geer, L. Y.; Geer, R. C.; Gonzales, N. R.; Gwadz, M.; Hurwitz, D. I.; Jackson, J. D.; Ke, Z.; Lanczycki, C. J.; Lu, F.; Marchler, G. H.; Mullokandov, M.; Omelchenko, M. V.; Robertson, C. L.; Song, J. S.; Thanki, N.; Yamashita, R. A.; Zhang, D.; Zhang, N.; Zheng, C.; Bryant, S. H. CDD: A conserved domain database for the functional annotation of proteins Nucleic Acids Res. 2011, 39, D225-D229
23. Katsuya, Y.; Mezaki, Y.; Kubota, M.; Matsuura, Y. Three-dimensional structure of Pseudomonas isoamylase at 2.2 A resolution J. Mol. Biol. 1998, 281, 885-897
24. Wiatrowski, H. A.; van Denderen, B. J. W.; Berkey, C. D.; Kemp, B. E.; Stapleton, D.; Carlson, M. Mutations in the Gal83 glycogen-binding domain activate the Snf1/Gal83 kinase pathway by a glycogen-independent mechanism Mol. Cell. Biol. 2004, 24, 352-361
25. Polekhina, G.; Gupta, A.; Michell, B. J.; van Denderen, B.; Murthy, S.; Feil, S. C.; Jennings, I. G.; Campbell, D. J.; Witters, L. A.; Parker, M. W.; Kemp, B. E.; Stapleton, D. AMPK beta subunit targets metabolic stress sensing to glycogen Curr. Biol. 2003, 13, 867-871
26. Hudson, E. R.; Pan, D. A.; James, J.; Lucocq, J. M.; Hawley, S. A.; Green, K. A.; Baba, O.; Terashima, T.; Hardie, D. G. A novel domain in AMP-activated protein kinase causes glycogen storage bodies similar to those seen in hereditary cardiac arrhythmias Curr. Biol. 2003, 13, 861-866
27. Gomes, I.; Gomes, J.; Steiner, W. Highly thermostable amylase and pullulanase of the extreme thermophilic eubacterium Rhodothermus marinus: Production and partial characterization Bioresour. Technol. 2003, 90, 207-214
28. Norman, B. E. A novel debranching enzyme for application in the glucose syrup industry Starch/Staerke 1982, 34, 340-346
29. Rodríguez-Sanoja, R.; Oviedo, N.; Sánchez, S. Microbial starch-binding domain Curr. Opin. Microbiol. 2005, 8, 260-267
30. Birte, S. Regional distant sequence homology between amylases, α-glucosidases and transglucanosylases FEBS Lett. 1988, 230, 72-76
31. Janse, B. J. H.; Steyn, A. J. C.; Pretorius, I. S. Regional sequence homologies in starch-degrading enzymes Curr. Genetics 1993, 24, 400-407
32. Hatada, Y.; Igarashi, K.; Ozaki, K.; Ara, K.; Hitomi, J.; Kobayashi, T.; Kawai, S.; Watabe, T.; Ito, S. Amino acid sequence and molecular structure of an alkaline amylopullulanase from Bacillus that hydrolyzes α-1,4 and α-1,6 linkages in polysaccharides at different active sites J. Biol. Chem. 1996, 271, 24075-24083
33. Lévȩque, E.; Janeček, Š.; Haye, B.; Belarbi, A. Thermophilic archaeal amylolytic enzymes Enzyme Microb. Technol. 2000, 26, 3-14
34. Nakajima, R.; Imanaka, T.; Aiba, S. Comparison of amino acid sequences of eleven different α-amylases Appl. Microbiol. Biotechnol. 1986, 23, 355-360
35. Mathupala, S. P.; Lowe, S. E.; Podkovyrov, S. M.; Zeikus, J. G. Sequencing of the amylopullulanase (apu) gene of Thermoanaerobacter ethanolicus 39E, and identification of the active site by site-directed mutagenesis J. Biol. Chem. 1993, 268, 16332-16344
36. Yamashita, M.; Matsumoto, D.; Murooka, Y. Amino acid residues specific for the catalytic action towards α-1,6-glucosidic linkages in Klebsiella pullulanase J. Ferment. Bioeng. 1997, 84, 283-290
37. Amemura, A.; Chakraborty, R.; Fujita, M.; Noumi, T.; Futai, M. Cloning and nucleotide sequence of the isoamylase gene from Pseudomonas amyloderamosa SB-15 J. Biol. Chem. 1988, 263, 9271-9275
38. Suzuki, Y.; Hatagaki, K.; Oda, H. A hyperthermostable pullulanase produced by an extreme thermophile, Bacillus flavocaldarius KP 1228, and evidence for the proline theory of increasing protein thermostability Appl. Microbiol. Biotechnol. 1991, 34, 707-714
39. Mikami, B.; Iwamoto, H.; Malle, D.; Yoon, H.-J.; Demirkan-Sarikaya, E.; Mezaki, Y.; Katsuya, Y. Crystal structure of pullulanase: Evidence for parallel binding of oligosaccharides in the active site J. Mol. Biol. 2006, 359, 690-707
40. Albertson, G. D.; McHale, R. H.; Gibbs, M. D.; Bergquist, P. L. Cloning and sequence of a type I pullulanase from an extremely thermophilic anaerobic bacterium, Caldicellulosiruptor saccharolyticus Biochim. Biophys. Acta, Gene Struct. Expression 1997, 1354, 35-39
41. D'Elia, J. N.; Salyers, A. A. Contribution of a neopullulanase, a pullulanase, and an alpha-glucosidase to growth of Bacteroides thetaiotaomicron on starch J. Bacteriol. 1996, 178, 7173-7179
42. Bibel, M.; Brettl, C.; Gosslar, U.; Kriegshäuser, G.; Liebl, W. Isolation and analysis of genes for amylolytic enzymes of the hyperthermophilic bacterium Thermotoga maritima FEMS Microbiol. Lett. 1998, 158, 9-15
43. Kriegshäuser, G.; Liebl, W. Pullulanase from the hyperthermophilic bacterium Thermotoga maritima: Purification by β-cyclodextrin affinity chromatography J. Chromatogr., B: Biomed. Sci. Appl. 2000, 737, 245-251
44. Kusano, S.; Shiraishi, T.; Takahashi, S.-I.; Fujimoto, D.; Sakano, Y. Immobilization of Bacillus acidopullulyticus pullulanase and properties of the immobilized pullulanases J. Ferment. Bioeng. 1989, 68, 233-237
45. Ben Messaoud, E.; Ben Ammar, Y.; Mellouli, L.; Bejar, S. Thermostable pullulanase type I from new isolated Bacillus thermoleovorans US105: Cloning, sequencing and expression of the gene in E. coli Enzyme Microb. Technol. 2002, 31, 827-832
46. Zouari Ayadi, D.; Ben Ali, M.; Jemli, S.; Ben Mabrouk, S.; Mezghani, M.; Ben Messaoud, E.; Bejar, S. Heterologous expression, secretion and characterization of the Geobacillus thermoleovorans US105 type I pullulanase Appl. Microbiol. Biotechnol. 2008, 78, 473-481
47. Ishiwa, H.; Tsuchida, N. New shuttle vectors for Escherichia coli and Bacillus subtilis. I. Construction and characterization of plasmid pHY460 with twelve unique cloning sites Gene 1984, 32, 129-134