Molecular cloning and biochemical characterization of a heat-stable type I pullulanase from Thermotoga neapolitana

Enzyme and Microbial Technology

Volumn 48, Issue 3, 2011, Pages 260-266

  Kang, Jinho ^a   Park, Kyung Min ^a   Choi, Kyoung Hwa ^a   Park, Cheon Seok ^b   Kim, Go Eun ^c   Kim, Doman ^c   Cha, Jaeho ^a  

^a Pusan National University   (South Korea)

^b Kyung Hee University   (South Korea)

^c Chonnam National University   (South Korea)

Author keywords

Hyperthermophiles; Thermotoga neapolitana; Transglycosylation; Type I pullulanase

Indexed keywords

AESCULIN; AMYLOLYTIC ENZYMES; ANAEROBIC BACTERIUM; BIOCHEMICAL CHARACTERIZATION; E. COLI; HYDROLYTIC ACTIVITIES; HYPERTHERMOPHILES; HYPERTHERMOPHILIC; ISOAMYLASE; MALTOTRIOSE; MOLECULAR CLONING; PULLULANASE; PULLULANS; RECOMBINANT ENZYMES; SIGNAL PEPTIDE; SUBSTRATE SPECIFICITY; THERMOTOGA MARITIMA; THERMOTOGANEAPOLITANA; TRANS GLYCOSYLATION ACTIVITY; TRANSGLYCOSYLATION; TYPE I PULLULANASE;

AMINO ACIDS; BACTERIA; CHROMATOGRAPHIC ANALYSIS; CLONING; ENCODING (SYMBOLS); ENZYME ACTIVITY; ENZYMES; ESCHERICHIA COLI; THIN LAYER CHROMATOGRAPHY;

GENE ENCODING;

AMYLOPECTIN; AMYLOSE; ESCULIN; GLYCOGEN; HYDROLASE; ISOAMYLASE; MALTOTRIOSE; PULLULAN; PULLULANASE; SIGNAL PEPTIDE; STARCH;

ARTICLE; CHEMICAL ANALYSIS; CHEMICAL STRUCTURE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME GLYCOSYLATION; ENZYME SPECIFICITY; ESCHERICHIA COLI; GENE OVEREXPRESSION; GENE SEQUENCE; HALF LIFE TIME; MOLECULAR CLONING; NONHUMAN; PH MEASUREMENT; PROMOTER REGION; THERMOTOGA MARITIMA; THERMOTOGA NEAPOLITANA;

AMINO ACID SEQUENCE; CLONING, MOLECULAR; ENZYME STABILITY; ESCHERICHIA COLI; GLUCANS; GLYCOSIDE HYDROLASES; GLYCOSYLATION; HOT TEMPERATURE; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA; SUBSTRATE SPECIFICITY; THERMOTOGA NEAPOLITANA;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; THERMOTOGA MARITIMA; THERMOTOGA NEAPOLITANA;

EID: 79551472575     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2010.11.006     Document Type: Article

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