Cigarette smoke-induced morphological transformation of bhas 42 cells In Vitro

Alternatives to Laboratory Animals

Volumn 41, Issue 2, 2013, Pages 181-189

  Weisensee, Dirk ^a,d   Poth, Albrecht ^b   Roemer, Ewald ^c   Conroy, Lynda L ^a   Schlage, Walter K ^a  

^a Philip Morris Research Laboratories GmbH   (Germany)

^b Harlan   (Switzerland)

^c PHILIP MORRIS PRODUCTS S A   (Switzerland)

^d CellSystems GmbH   (Germany)

Author keywords

Bhas 42; Cell transformation; Cigarette smoke; In vitro; Total particulate matter; Tumour promotion

Indexed keywords

CIGARETTE SMOKE;

ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; BHAS 42 CELL; CARCINOGENESIS; CARCINOGENIC ACTIVITY; CELL ASSAY; CELL STRAIN 3T3; CELL TRANSFORMATION; FIBROBLAST; IN VITRO STUDY; MOUSE; NONHUMAN; PARTICULATE MATTER; PRIORITY JOURNAL; REPRODUCIBILITY;

NICOTIANA TABACUM;

EID: 84881105135     PISSN: 02611929     EISSN: None     Source Type: Journal    
DOI: 10.1177/026119291304100207     Document Type: Article

References (50)

1. IARC/NCI/EPA Working Group (1985). Cellular and molecular mechanisms of cell transformation and standardization of transformation assays of established cell lines for the prediction of carcinogenic chemicals: Overview and recommended protocols. Cancer Research 45, 2395-2399.
2. Breheny, D., Oke, O. & Faux, S.P. (2011). The use of in vitro systems to assess cancer mechanisms and the carcinogenic potential of chemicals. ATLA 39, 233-255.
3. Schechtman, L.M. (2012). Rodent cell transformation assaysa brief historical perspective. Mutation Research 744, 3-7.
4. Landolph, J.R. (1985). Chemical transformation in C3H 10T1/2 CL8 mouse embryo fibroblasts: Historical background, assessment of the transformation assay, and evolution and optimization of the transformation assay protocol. IARC Scientific Publications 67, 185-203.
5. Reznikoff, C.A., Bertram, J.S., Brankow, D.W. & Heidelberger, C. (1973). Quantitative and qualitative studies of chemical transformation of cloned C3H mouse embryo cells sensitive to postconfluence inhibition of cell division. Cancer Research 33, 3239-3249.
6. Frazelle, J.H., Abernethy, D.J. & Boreiko, C.J. (1983). Weak promotion of C3H/10T1/2 cell transformation by repeated treatments with formaldehyde. Cancer Research 43, 3236-3239.
7. Frazelle, J.H., Abernethy, D.J. & Boreiko, C.J. (1984). Enhanced sensitivity of the C3H/10T1/2 cell transformation system to alkylating and chemotherapeutic agents by treatment with 12-O-Tetradecanoylphorbol-13-Acetate. Environmental Mutagenesis 6, 81-89.
8. Mondal, S., Heidelberger, C. & Brankow, D.W. (1976). Two-stage chemical oncogenesis in cultures of C3H/10T1/2 cells. Cancer Research 36, 2254-2260.
9. Kerckaert, G.A., Brauninger, R., Leboeuf, R.A. & Isfort, R.J. (1996). Use of the Syrian hamster embryo cell transformation assay for carcinogenicity prediction of chemicals currently being tested by the National Toxicology Program in rodent bioassays. Environmental Health Perspectives 104, Suppl. 5, 1075-1084.
10. LeBoeuf, R.A., Kerckaert, K.A., Aardema, M.J. & Isfort, R.J. (1999). Use of Syrian hamster embryo and BALB/c 3T3 cell transformation for assessing the carcinogenic potential of chemicals. IARC Scientific Publications 146, 409-425.
11. Combes, R., Balls, M., Curren, R., Fischbach, M., Fusenig, N., Kirkland, D., Lasne, C., Landolph, J., LeBoeuf, R., Marquardt, H., McCormick, J., Müller, L., Rivedal, E., Sabbioni, E., Tanaka, N., Vasseur, P. & Yamasaki, H. (1999). Cell transformation assays as predictors of human carcinogenicity. The report and recommendations of ECVAM Workshop 39. ATLA 27, 745-767.
12. Yamasaki, H., Ashby, J., Bignami, M., Jongen, W., Linnainmaa, K., Newbold, R.F., Nguyen-Ba, G., Parodi, S., Rivedal, E., Schiffmann, D., Simon, J.W.I.M. & Vasseur, P. (1996). Nongenotoxic carcinogens: Development of detection methods based on mechanisms: A European project. Mutation Research 353, 47-63.
13. Vasseur, P. & Lasne, C. (2011). OECD Detailed Review Paper (DRP) number 31 on "Cell Transformation Assays for Detection of Chemical Carcinogens": Main results and conclusions. Mutation Research 744, 8-11.
14. Corvi, R., Aardema, M.J., Gribaldo, L., Hayashi, M., Hoffmann, S., Schechtman, L. & Vanparys, P. (2011). ECVAM prevalidation study on in vitro cell transformation assays: General outline and conclusions of the study. Mutation Research 744, 12-19.
15. IARC (1986). IARC Monographs on the Evaluation of the Carcinogenic Risk of Chemicals to Humans. Tobacco Smoking, pp. 127-198. Lyon, France: International Agency for Research on Cancer.
16. Aarsaether, N., Lillehaug, J.R., Rivedal, E. & Sanner, T. (1987). Cell transformation and promoter activity of insulation oils in the Syrian hamster cell and in the C3H/10T1/2 mouse embryo fibroblast test systems. Journal of Toxicology & Environmental Health 20, 173-185.
17. Nelson, R.L. & Garry, V.F. (1983). Colony transformation in C3H 10T1/2 cells: Stepwise development of neoplastic change in vitro. In Vitro 19, 551-558.
18. Mauthe, R.J., Gibson, D.P., Bunch, R.T. & Custer, L. (2001). The Syrian hamster embryo (SHE) cell transformation assay: Review of the methods and results. Toxicologic Pathology 29, Suppl., 138-146.
19. Sakai, A., Iwase, Y., Nakamura, Y., Sasaki, K., Tanaka, N. & Umeda, M. (2002). Use of a cell transformation assay with established cell lines, and a metabolic cooperation assay with V79 cells for the detection of tumour promoters: A review. ATLA 30, 33-59.
20. Muramatsu, D., Sasaki, K., Kuroda, S., Hayashi, K., Tanaka, N. & Sakai, A. (2009). Comparison of sensitivity to arsenic compounds between a Bhas 42 cell transformation assay and a BALB/c 3T3 cell transformation assay. Mutation Research 675, 66-70.
21. Benigni, R. & Zito, R. (2004). The second National Toxicology Program comparative exercise on the prediction of rodent carcinogenicity: Definitive results. Mutation Research 566, 49-63.
22. Dunkel, V.C., Pienta, R.J., Sivak, A. & Traul, K.A. (1981). Comparative neoplastic transformation responses of Balb/3T3 cells, Syrian hamster embryo cells, and Rauscher murine leukemia virus-infected Fischer 344 rat embryo cells to chemical compounds. Journal of the National Cancer Institute 67, 1303-1312.
23. Aardema, M.J., Isfort, R.J., Thompson, E.D. & Leboeuf, R.A. (1996). The low pH Syrian hamster embryo (SHE) cell transformation assay: A revitalized role in carcinogen prediction. Mutation Research 356, 5-9.
24. Matthews, E.J., Spalding, J.W. & Tennant, R.W. (1993). Transformation of BALB/c-3T3 cells: IV. Rank-ordered potency of 24 chemical responses detected in a sensitive new assay procedure. Environmental Health Perspectives 101, Suppl. 2, 319-345.
25. Smith, C.J., Perfetti, T.A., Garg, R. & Hansch, C. (2006). Utility of the mouse dermal promotion assay in comparing the tumorigenic potential of cigarette mainstream smoke. Food & Chemical Toxicology 44, 1699-1706.
26. Walaszek, Z., Hanausek, M. & Slaga, T.J. (2007). The role of skin painting in predicting lung cancer. International Journal of Toxicology 26, 345-351.
27. Werley, M.S., Freelin, S.A., Wrenn, S.E., Gerstenberg, B., Roemer, E., Schramke, H., Van Miert, E., Vanscheeuwijck, P., Weber, S. & Coggins, C.R. Smoke chemistry, in vitro and in vivo toxicology evaluations of the electrically heated cigarette smoking system series K. Regulatory Toxi cology & Pharmacology 52, 122-139.
28. Roemer, E., Ottmueller, T.H., Zenzen, V., Wittke, S., Radtke, F., Blanco, I. & Carchman, R.A. (2009). Cytotoxicity, mutagenicity, and tumorigenicity of mainstream smoke from three reference cigarettes machine-smoked to the same yields of total particulate matter per cigarette. Food & Chemical Toxicology 47, 1810-1818.
29. Roemer, E., Ottmueller, T.H., Urban, H.J. & Baillet-Mignard, C. (2010). SKH-1 mouse skin painting: A short-Term assay to evaluate the tumorigenic activity of cigarette smoke condensate. Toxicology Letters 192, 155-161.
30. Benedict, W.F., Rucker, N., Faust, J. & Kouri, R.E. (1975). Malignant transformation of mouse cells by cigarette smoke condensate. Cancer Research 35, 857-860.
31. Langenfeld, J., Lonardo, F., Kiyokawa, H., Passalaris, T., Ahn, M.J., Rusch, V. & Dmitrovsky, E. (1996). Inhibited transformation of immortalized human bronchial epithelial cells by retinoic acid is linked to cyclin E down-regulation. Oncogene 13, 1983-1990.
32. Breheny, D., Zhang, H. & Massey, E.D. (2005). Application of a two-stage Syrian hamster embryo cell transformation assay to cigarette smoke particulate matter. Mutation Research 572, 45-57.
33. Schlage, W.K., Buelles, H., Friedrichs, D. & Kurkowsky, B. (1999). Two-stage transformation assay for cigarette smoke condensates using murine C3H-10T1/2 fibroblasts. Toxicology in Vitro 13, 823-828.
34. Rivedal, E. & Sanner, T. (1980). Potentiating effect of cigarette smoke extract on morphological transformation of hamster embryo cells by benzo[a]pyrene. Cancer Letters 10, 193-198.
35. Sasaki, K., Mizusawa, H., Ishidate, M. & Tanaka, N. (1990). Transformation of ras transfected BALB 3T3 clone (Bhas 42) by promoters: Application for screening and specificity of promoters. Toxicology in Vitro 4, 657-659.
36. Ohmori, K., Umeda, M., Tanaka, N., Takagi, H., Yoshimura, I., Sasaki, K., Asasda, S., Sakai, A., Araki, H., Asakura, M., Baba, H., Fushiwaki, Y., Hamada, S., Kitou, N., Nakamura, T., Nakamura, Y., Oishi, H., Sasaki, S., Shimada, S., Tsuchiya, T., Uno, Y., Washizuka, M., Yajima, S., Yamamoto, Y., Yamamura, E. & Yatsushiro, T. (2005). An interlaboratory collaborative study by the Non-Genotoxic Carcinogen Study Group in Japan, on a cell transformation assay for tumour promoters using Bhas 42 cells. ATLA 33, 619-639.
37. Ohmori, K., Sasaki, K., Asada, S., Tanaka, N. & Umeda, M. (2004). An assay method for the prediction of tumor promoting potential of chemicals by the use of Bhas 42 cells. Mutation Research 557, 191-202.
38. Asada, S., Sasaki, K., Tanaka, N., Takeda, K., Hayashi, M. & Umeda, M. (2005). Detection of initiating as well as promoting activity of chemicals by a novel cell transformation assay using v-Ha-rastransfected BALB/c 3T3 cells (Bhas 42 cells). Mutation Research 588, 7-21.
39. Sakai, A., Sasaki, K., Muramatsu, D., Arai, S., Endou, N., Kuroda, S., Hayashi, K., Lim, Y.M., Yamazaki, S., Umeda, M. & Tanaka, N. (2010). A Bhas 42 cell transformation assay on 98 chemicals: The characteristics and performance for the prediction of chemical carcinogenicity. Mutation Research 702, 100-122.
40. Sakai, A., Sasaki, K., Hayashi, K., Muramatsu, D., Arai, S., Endou, N., Kuroda, S., Poth, A., Bohnenberger, S., Kunkelmann, T., Asakura, M., Hirose, H., Ishii, N., Mizuhashi, F., Kasamoto, S., Nagai, M., Pant, K., Bruce, S.W., Sly, J.E., Yamazaki, S., Umeda, M. & Tanaka, N. (2011). An international validation study of a Bhas 42 cell transformation assay for the prediction of chemical carcinogenicity. Mutation Research 725, 57-77.
41. Muramatsu, D., Sasaki, K., Kuroda, S., Hayashi, K., Tanaka, N. & Sakai, A. (2009). Comparison of sensitivity to arsenic compounds between a Bhas 42 cell transformation assay and a BALB/c 3T3 cell transformation assay. Mutation Research 675, 66-70.
42. University of Kentucky (2013). Reference Cigarette Program. Lexington, KY, USA: The University of Kentucky Printing Services. Available at: http://www.ca.uky.edu/refcig/(Accessed 05.04.13
43. ISO (2000). ISO 3308, Routine Analytical Cigarettesmoking MachineDefinitions and Standard Conditions, 25pp. Geneva, Switzerland: International Organisation for Standardisation.
44. Aaronson, S.A. & Todaro, G.J. (1968). Development of 3T3-like lines from Balb-c mouse embryo cultures: Transformation susceptibility to SV40. Journal of Cell Physiology 72, 141-148.
45. Kakunaga, T. (1973). A quantitative system for assay of malignant transformation by chemical carcinogens using a clone derived from BALB-3T3. International Journal of Cancer 12, 463-473.
46. Sasaki, K., Mizusawa, H. & Ishidate, M. (1988). Isolation and characterization of ras-Transfected BALB/3T3 clone showing morphological transformation by 12-O-Tetradecanoyl-phorbol-13-Acetate. Japanese Journal of Cancer Research 79, 921-930.
47. ISO (1994). ISO 5725-2, Accuracy (Trueness and Precision) of Measurement Methods and ResultsPart 2: A Basic Method for the Determination of Repeatability and Reproducibility of a Standard Measurement Method, 42pp. Geneva, Switzerland: International Organisation for Standardisation.
48. Tuomela, M., Stanescu, I. & Krohn, K. (2005). Validation overview of bio-Analytical methods. Gene Therapy 12, S131-S138.
49. Schramke, H., Meisgen, T.J., Tewes, F.J., Gomm, W. & Roemer, E. (2006). The mouse lymphoma thymidine kinase assay for the assessment and comparison of the mutagenic activity of cigarette mainstream smoke particulate phase. Toxicology 227, 193-210.
50. Combes, R.D. (2012). Cell transformation assays: Are we barking up the wrong tree? ATLA 40, 115-130.