A fluorescence-based assay for the apurinic/apyrimidinic-site cleavage activity of human tyrosyl-DNA phosphodiesterase 1

Analytical Biochemistry

Volumn 440, Issue 1, 2013, Pages 1-5

  Thomson, Graeme J ^a   Hamilton, Nicola S ^a   Hopkins, Gemma V ^a   Waddell, Ian D ^a   Watson, Amanda J ^a   Ogilvie, Donald J ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

Assay; DNA repair; High throughput screen; Inhibitor; Tdp1; Topoisomerase

Indexed keywords

AMINO ACIDS; ASSAYS; ESTERS;

DNA REPAIR; HIGH-THROUGHPUT SCREENS; INHIBITOR; TDP1; TOPOISOMERASES;

DNA;

APURINIC ACID; PHOSPHOLIPASE D; TYROSYL DNA PHOSPHODIESTERASE 1; TYROSYL DNA PHOSPHODIESTERASE 2; UNCLASSIFIED DRUG;

APYRIMIDINIC SITE; ARTICLE; CHEMICAL STRUCTURE; CONTROLLED STUDY; DNA REPAIR; ENZYME INHIBITION; ESCHERICHIA COLI; FLUORESCENCE; FLUORESCENCE POLARIZATION; HIGH THROUGHPUT SCREENING; MICHAELIS MENTEN KINETICS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN TARGETING; REACTION TIME;

EID: 84879487318     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2013.05.003     Document Type: Article

References (26)

1. J.C. Wang, DNA topoisomerases, Annu. Rev. Biochem. 65 (1996) 635-692. (Pubitemid 26250622)
2. Y.Y. Pommier, Drugging topoisomerases: lessons and challenges, ACS Chem. Biol. 8 (2013) 82-95.
3. M.C. Rideout, A.C. Raymond, A.B. Burgin Jr., Design and synthesis of fluorescent substrates for human tyrosyl-DNA phosphodiesterase 1, Nucleic Acids Res. 32 (2004) 4657-4664. (Pubitemid 39317742)
4. S.W. Yang, A.B. Burgin Jr., B.N. Huizenga, C.A. Robertson, K.C. Yao, H.A. Nash, A eukaryotic enzyme that can disjoin dead-end covalent complexes between DNA and type I topoisomerases, Proc. Natl. Acad. Sci. U.S.A. 93 (1996) 11534-11539. (Pubitemid 26347156)
5. J.J. Pouliot, K.C. Yao, C.A. Robertson, H.A. Nash, Yeast gene for a Tyr-DNA phosphodiesterase that repairs topoisomerase I complexes, Science 286 (1999) 552-555.
6. H. Interthal, H.J. Chen, T.E. Kehl-Fie, J. Zotzmann, J.B. Leppard, J.J. Champoux, SCAN1 mutant Tdp1 accumulates the enzyme-DNA intermediate and causes camptothecin hypersensitivity, EMBO J. 24 (2005) 2224-2233. (Pubitemid 40961806)
7. J. Murai, S.N. Huang, B. Brata Das, T.S. Dexheimer, S. Takeda, Y. Pommier, Tyrosyl-DNA phosphodiesterase 1 (TDP1) repairs DNA damage induced by topoisomerases I and II and base alkylation in vertebrate cells, J. Biol. Chem. 287 (2012) 12848-12857.
8. H.U. Barthelmes, M. Habermeyer, M.O. Christensen, C. Mielke, H. Interthal, J.J. Pouliot, F. Boege, D. Marko, TDP1 overexpression in human cells counteracts DNA damage mediated by topoisomerases I and II, J. Biol. Chem. 279 (2004) 55618-55625. (Pubitemid 40066566)
9. C. Liu, S. Zhou, S. Begum, D. Sidransky, W.H. Westra, M. Brock, J.A. Califano, Increased expression and activity of repair genes TDP1 and XPF in non-small cell lung cancer, Lung Cancer 55 (2007) 303-311. (Pubitemid 46329184)
10. Z. Liao, L. Thibaut, A. Jobson, Y. Pommier, Inhibition of human tyrosyl-DNA phosphodiesterase by aminoglycoside antibiotics and ribosome inhibitors, Mol. Pharmacol. 70 (2006) 366-372.
11. S. Antony, C. Marchand, A.G. Stephen, L. Thibaut, K.K. Agama, R.J. Fisher, Y. Pommier, Novel high-Throughput electrochemiluminescent assay for identification of human tyrosyl-DNA phosphodiesterase (Tdp1) inhibitors and characterization of furamidine (NSC 305831) as an inhibitor of Tdp1, Nucleic Acids Res. 35 (2007) 4474-4484. (Pubitemid 47244598)
12. C. Marchand, W.A. Lea, A. Jadhav, T.S. Dexheimer, C.P. Austin, J. Inglese, Y. Pommier, A. Simeonov, Identification of phosphotyrosine mimetic inhibitors of human tyrosyl-DNA phosphodiesterase I by a novel AlphaScreen highthroughput assay, Mol. Cancer Ther. 8 (2009) 240-248.
13. M. Takagi, J. Ueda, J.H. Hwang, J. Hashimoto, M. Izumikawa, H. Murakami, Y. Sekido, K. Shin-ya, Tyrosyl-DNA phosphodiesterase 1 inhibitor from an anamorphic fungus, J. Nat. Prod. 75 (2012) 764-767.
14. M. Conda-Sheridan, P.V. Reddy, A. Morrell, B.T. Cobb, C. Marchand, K. Agama, A. Chergui, A. Renaud, A.G. Stephen, L.K. Bindu, Y. Pommier, M. Cushman, Synthesis and biological evaluation of indenoisoquinolines that inhibit both tyrosyl-DNA phosphodiesterase I (Tdp1) and topoisomerase I (Top1), J. Med. Chem. 56 (2013) 182-200.
15. V.R. Sirivolu, S.K.V. Vernekar, C. Marchand, A. Naumova, A. Chergui, A. Renaud, A.G. Stephen, F. Chen, Y.Y. Sham, Y. Pommier, Z. Wang, 5-Arylidenethioxothiazolidinones as inhibitors of tyrosyl-DNA phosphodiesterase I, J. Med. Chem. 55 (2012) 8671-8684.
16. H. Interthal, H.J. Chen, J.J. Champoux, Human Tdp1 cleaves a broad spectrum of substrates, including phosphoamide linkages, J. Biol. Chem. 280 (2005) 36518-36528. (Pubitemid 41633926)
17. N.A. Lebedeva, N.I. Rechkunova, O.I. Lavrik, AP-site cleavage activity of tyrosyl-DNA phosphodiesterase 1, FEBS Lett. 585 (2011) 683-686.
18. N.A. Lebedeva, N.I. Rechkunova, S.F. El-Khamisy, O.I. Lavrik, Tyrosyl-DNA phosphodiesterase 1 initiates repair of apurinic/apyrimidinic sites, Biochimie 94 (2012) 1749-1753.
19. G. Thomson, A. Watson, K. Caldecott, O. Denneny, P. Depledge, N. Hamilton, G. Hopkins, A. Jordan, C. Morrow, A. Raoof, I. Waddell, D. Ogilvie, Generation of assays and antibodies to facilitate the study of human 50-Tyrosyl DNA phosphodiesterase, Anal. Biochem. 436 (2013) 145-150.
20. T.J. Cheng, P.G. Rey, T. Poon, C.C. Kan, Kinetic studies of human tyrosyl-DNA phosphodiesterase, an enzyme in the topoisomerase I DNA repair pathway, Eur. J. Biochem. 269 (2002) 3697-3704. (Pubitemid 34989118)
21. T.S. Dexheimer, A.G. Stephen, M.J. Fivash, R.J. Fisher, Y. Pommier, The DNA binding and 30-end preferential activity of human tyrosyl-DNA phosphodiesterase, Nucleic Acids Res. 38 (2010) 2444-2452.
22. A. Simeonov, A. Kulkarni, D. Dorjsuren, A. Jadhav, M. Shen, D.R. McNeill, C.P. Austin, D.M. Wilson 3rd, Identification and characterization of inhibitors of human apurinic/apyrimidinic endonuclease APE1, PLoS One 4 (2009) e5740.
23. A. Simeonov, A. Jadhav, C.J. Thomas, Y. Wang, R. Huang, N.T. Southall, P. Shinn, J. Smith, C.P. Austin, D.S. Auld, J. Inglese, Fluorescence spectroscopic profiling of compound libraries, J. Med. Chem. 51 (2008) 2363-2371. (Pubitemid 351628498)
24. A.C. Raymond, B.L. Staker, A.B. Burgin Jr., Substrate specificity of tyrosyl-DNA phosphodiesterase I (Tdp1), J. Biol. Chem. 280 (2005) 22029-22035. (Pubitemid 40827857)
25. J.H. Zhang, T.D. Chung, K.R. Oldenburg, A simple statistical parameter for use in evaluation and validation of high throughput screening assays, J. Biomol. Screen. 4 (1999) 67-73. (Pubitemid 29278954)
26. A. Raoof, P. Depledge, N.S. Hamilton, J.R. Hitchin, G.V. Hopkins, A.M. Jordan, L.A. Maguire, A.E. McGonagle, D.P. Mould, M. Rushbrooke, H.F. Small, K.M. Smith, G.J. Thomson, F. Turlais, I.D. Waddell, B. Waszkowycz, M.J. Watson, D.J. Ogilvie, Toxoflavins and deazaflavins as the first reported small molecule inhibitors of tyrosyl-DNA phosphodiesterase II, J. Med. Chem. (2013) (submitted).