Heterologous expression and characterization of bacterial 2-C-methyl-d-erythritol-4-phosphate pathway in Saccharomyces cerevisiae

Applied Microbiology and Biotechnology

Volumn 97, Issue 13, 2013, Pages 5753-5769

  Carlsen, Simon ^a,b   Ajikumar, Parayil Kumaran ^b,d   Formenti, Luca Riccardo ^a   Zhou, Kang ^b,c   Phon, Too Heng ^c   Nielsen, Michael Lynge ^a,e   Lantz, Anna Eliasson ^a   Kielland Brandt, Morten C ^a   Stephanopoulos, Gregory ^b  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^b Massachusetts Institute of Technology Cambridge   (United States)

^c SINGAPORE MIT ALLIANCE   (Singapore)

^d Manus Biosynthesis   (United States)

^e NOVOZYMES A S   (Denmark)

Author keywords

Heterologous expression; Iron sulfur cluster proteins; MEP pathway; Metabolic engineering; S. cerevisiae; Terpenoids

Indexed keywords

HETEROLOGOUS EXPRESSION; IRON-SULFUR CLUSTERS; MEP PATHWAYS; S.CEREVISIAE; TERPENOIDS;

ENCODING (SYMBOLS); ESCHERICHIA COLI; EXPERIMENTS; GENES; IRON; MACHINERY; METABOLIC ENGINEERING; METABOLITES; PROTEINS; SULFUR;

YEAST;

2 C METHYL DEXTRO ERYTHRITOL 4 PHOSPHATE; ERYTHRITOL; GLUCOSE; IRON SULFUR PROTEIN; ISOMERASE; ISOPROPYLMALATE ISOMERASE; MEVALONIC ACID; UNCLASSIFIED DRUG;

ANOXIC CONDITIONS; BIOENGINEERING; COLIFORM BACTERIUM; GENOME; IRON; PROTEIN; SECONDARY METABOLITE; SULFUR; TERPENE; YEAST;

ARTICLE; AUXOTROPHY; BACTERIAL GENE; BACTERIAL GROWTH; CONTROLLED STUDY; CYTOSOL; ESCHERICHIA COLI; GENE EXPRESSION; MASS SPECTROMETRY; NONHUMAN; SACCHAROMYCES CEREVISIAE; SYNTHESIS; ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY;

BIOSYNTHETIC PATHWAYS; CHROMATOGRAPHY, LIQUID; DNA, BACTERIAL; ERYTHRITOL; ESCHERICHIA COLI; GENE EXPRESSION; IRON-SULFUR PROTEINS; MASS SPECTROMETRY; METABOLIC ENGINEERING; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; SEQUENCE ANALYSIS, DNA; SUGAR PHOSPHATES;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; SACCHAROMYCES CEREVISIAE;

EID: 84879416874     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-013-4877-y     Document Type: Article

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