Xylose isomerase overexpression along with engineering of the pentose phosphate pathway and evolutionary engineering enable rapid xylose utilization and ethanol production by Saccharomyces cerevisiae

Metabolic Engineering

Volumn 14, Issue 6, 2012, Pages 611-622

  Zhou, Hang ^a   Cheng, Jing sheng ^a   Wang, Benjamin L ^a   Fink, Gerald R ^b   Stephanopoulos, Gregory ^a  

^a Massachusetts Institute of Technology Cambridge   (United States)

^b WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH   (United States)

Author keywords

Ethanol production; Evolutionary engineering; Inverse metabolic engineering; Saccharomyces cerevisiae; Xylose isomerase; Xylose utilization

Indexed keywords

ANAEROBIC GROWTH; COMPLEMENTATION; CONSUMPTION RATES; ETHANOL CONVERSION; ETHANOL PRODUCTION; EVOLUTIONARY ENGINEERING; EXPRESSION LEVELS; INVERSE METABOLIC ENGINEERING; LIGNOCELLULOSIC FEEDSTOCKS; METABOLIC ENGINEERING APPROACH; NON-OXIDATIVE; OVER-EXPRESSION; PENTOSE PHOSPHATE PATHWAY; PERFORMANCE METRICS; PICHIA STIPITIS; S.CEREVISIAE; SEQUENTIAL BATCH; THREE-STAGE PROCESS; XYLOSE ISOMERASE; XYLULOSE; YEAST SACCHAROMYCES CEREVISIAE;

ETHANOL; GENES; METABOLIC ENGINEERING;

YEAST;

ALCOHOL PRODUCTION; ARTICLE; CHEMOSTAT; ENZYME ENGINEERING; FUNGAL GENE; FUNGAL STRAIN; GENE OVEREXPRESSION; GROWTH RATE; METABOLIC ENGINEERING; NONHUMAN; PENTOSE PHOSPHATE CYCLE; PICHIA STIPITIS; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; XYLOSE ISOMERASE GENE; XYLULOSE KINASE GENE;

ALDOSE-KETOSE ISOMERASES; DIRECTED MOLECULAR EVOLUTION; ETHANOL; GENETIC ENHANCEMENT; PENTOSE PHOSPHATE PATHWAY; PHOSPHOTRANSFERASES (ALCOHOL GROUP ACCEPTOR); PICHIA; PIROMYCES; PROTEIN ENGINEERING; SACCHAROMYCES CEREVISIAE; UP-REGULATION; XYLOSE;

PICHIA STIPITIS; PIROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 84869043924     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2012.07.011     Document Type: Article

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