Purification and antibacterial activity of recombinant warnericin RK expressed in Escherichia coli

Applied Microbiology and Biotechnology

Volumn 97, Issue 12, 2013, Pages 5401-5412

  Verdon, Julien ^a   Girardin, Nicolas ^a   Marchand, Adrienne ^a   Héchard, Yann ^a   Berjeaud, Jean Marc ^a  

^a UNIVERSITÉ DE POITIERS   (France)

Author keywords

Antimicrobial peptide; Circular dichroism; Fusion peptide; Heterologous expression; Legionella; Peptide purification

Indexed keywords

ANTIMICROBIAL PEPTIDE; FUSION PEPTIDES; HETEROLOGOUS EXPRESSION; LEGIONELLA; MEMBRANE-ACTIVE PEPTIDES; PURIFICATION PROCEDURES; SOLID-STATE NMR SPECTROSCOPY; STRUCTURAL MODIFICATIONS;

AFFINITY CHROMATOGRAPHY; DICHROISM; ESCHERICHIA COLI; ISOTOPES; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PURIFICATION;

PEPTIDES;

ANTIINFECTIVE AGENT; COMPLEMENTARY DNA; DNA FRAGMENT; NITROGEN 15; PEPTIDE; POLYHISTIDINE TAG; UNCLASSIFIED DRUG; WARNERICIN RK;

ANTIMICROBIAL ACTIVITY; COLIFORM BACTERIUM; GENE EXPRESSION; NUCLEAR MAGNETIC RESONANCE; PEPTIDE; PURIFICATION;

AFFINITY CHROMATOGRAPHY; ANTIBACTERIAL ACTIVITY; ARTICLE; BACTERIAL CELL; BACTERICIDAL ACTIVITY; CELL INCLUSION; CONTROLLED STUDY; DNA SEQUENCE; DRUG PURIFICATION; ESCHERICHIA COLI; EXPRESSION VECTOR; GROWTH INHIBITION; LEGIONELLA; MASS SPECTROMETRY; MINIMUM INHIBITORY CONCENTRATION; MOLECULAR WEIGHT; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SOLID STATE; SOLUBILIZATION; STAPHYLOCOCCUS WARNERI;

BACTERIOCINS; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC VECTORS; LEGIONELLA; RECOMBINANT PROTEINS;

ESCHERICHIA COLI; LEGIONELLA; STAPHYLOCOCCUS WARNERI;

EID: 84878734229     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-012-4417-1     Document Type: Article

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