Cloning, expression, and pharmacological activity of BmK AS, an active peptide from scorpion Buthus martensii Karsch

Biotechnology Letters

Volumn 30, Issue 1, 2008, Pages 23-29

  Shao, Jian Hua ^a   Wang, Yue Qiu ^a   Wu, Xiao Yan ^a   Jiang, Rui ^a   Zhang, Rong ^a   Wu, Chun Fu ^a   Zhang, Jing Hai ^a  

^a SHENYANG PHARMACEUTICAL UNIVERSITY   (China)

Author keywords

Analgesic activity; Antimicrobial activity; BmK AS; Functional expression; Homology model

Indexed keywords

ANALGESIC ACTIVITY; ANTIMICROBIAL ACTIVITY; BUTHUS MARTENSII KARSCH (BMK); FUNCTIONAL EXPRESSION; HOMOLOGY MODELS;

BACTERIA; CLONING; ESCHERICHIA COLI; GENE EXPRESSION; PHARMACODYNAMICS;

PEPTIDES;

ANALGESIC AGENT; ANTIINFECTIVE AGENT; BMK AS POLYPEPTIDE; PEPTIDE; RECOMBINANT PROTEIN; SCORPION VENOM; UNCLASSIFIED DRUG;

ANIMAL; ARTICLE; BACTERIUM; CHEMISTRY; DRUG EFFECT; ESCHERICHIA COLI; GENETICS; METABOLISM; MOLECULAR CLONING; PAIN THRESHOLD; SCORPION;

ANALGESICS; ANIMALS; ANTI-INFECTIVE AGENTS; BACTERIA; CLONING, MOLECULAR; ESCHERICHIA COLI; PAIN THRESHOLD; PEPTIDES; RECOMBINANT PROTEINS; SCORPION VENOMS; SCORPIONS;

ESCHERICHIA COLI; MESOBUTHUS MARTENSII; NEGIBACTERIA; POSIBACTERIA; SCORPIONES;

EID: 36649000008     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-007-9499-y     Document Type: Article

References (24)

1. An NL, Zhang ZQ, Wang S (1999) Coexpression of thioredoxin increased the solubility of foreign protein. Chin J Virol 15:131-135
2. Bignami GS (1993) A rapid and sensitive hemolysis neutralization assay for polytoxin. Toxicon 31:817-820
3. Chen B, Ji YH (2002) Antihyperalgesia effect of BmK AS, a scorpion toxin, in rat by intraplantar injection. Brain Res 952:322-326
4. + currents in primary afferent neurons. Peptides 27:2182-2192
5. Cociancich S, Goy M, Bontems F (1993) Purifcation and characterization of a scorpion defensin, a 4 kDa antibacterial peptide presenting structural similarities with insect defensins and scorpion toxins. Biochem Biophys Res Commun 194:17-22
6. Fennessy MR, Lee JR (1975) Methods in Narcotics Research. In: Ehrenpreis S, Neidle A (eds) Marcel Dekker, New York, pp 76-79
7. Goudet C, Chi CW, Tytgat J (2002) An overview of toxins and genes from the venom of the Asian scorpion Buthus martensi Karsch. Toxicon 40:1239-1258
8. Guex N, Peitsch MC (1997) SWISS-MODEL and the Swiss-PdbViewer: an environment for comparative protein modeling. Electrophoresis 18:2714-2723
9. Hancock REW (1997) Peptide antibiotics. Lancet 349:418-422
10. 3H] noradrenaline release from rat hippocampal slices. Biomed Res 3:257-260
11. Ji YH, Li YJ, Zhang JW (1999) Covalent structures of BmK AS and BmK AS-1, two novel bioactive polypeptides purified from Chinese scorpion Buthus martensi Karsch. Toxicon 37:519-536
12. Ji YH, Yan L, Xu K (1997b) BmK AS, amino acid sequence of a novel activator of ryanodine receptor on skeletal muscle. Chin Sci Bull 42:952-956
13. Kane JF, Hartley DL (1988) Formation of recombinant protein inclusion bodies in E. coli. Trends Biotechnol 6:95-101
14. Laurence ES, Damarys L, Goyffon M (1996) Characterization of novel cysteine-rich antimicrobial peptides from scorpion blood. J Biol Chem 271:29537-29544
15. Lee W, Jablonsky MJ, Watt DD (1994) Proton nuclear magnetic resonance and distance geometry/simulated annealing studies on the variant-1 neurotoxin from the new world scorpion Centruroides sculpturatus Ewing. Biochemistry 33:2468-2475
16. Leea K, Song YS, Kima K (2004) Antibiotic activity and structural analysis of the scorpion-derived antimicrobial peptide IsCT and its analogs. Biochem Biophys Res Commun 323:712-719
17. Li YJ, Liu Y, Ji YH (1999) Characteristics of BmK AS to Rat Brain and Cockroach Nerve Cord Synaptosomes. Acta biochimica biophysica sinica 31:604-606
18. Li YJ, Liu Y, Ji YH (2000) BmK AS: new scorpion neurotoxin binds to distinct receptor sites of mammal and insect voltage-gated sodium channels. J Neurosci Res 61:541-548
19. Maitz MF, Poon RW, Liu XY, Pham MT, Chu PK (2005) Bioactiving of titanium following sodium plasma imonersion ion implantation and deposition. Biomaterials 26:5465-74
20. O'Czllagpan JP, Holtzman SG (1975) Quantification of the analgesic activity of narcotic antagonists by a modified hot-plate procedure. J Pharmacol Exp Ther 192:497-505
21. +-channels. Eur J Biochem 264:287-300
22. Rodríguez RC, García BI, Ambrosio CD (2004) Antimicrobial peptide induction in the haemolymph of the Mexican scorpion Centruroides limpidus limpidusin response to septic injury. Cell Mol Life Sci 61:1507-1509
23. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
24. Yasukawa T, Kanei-Ishii C, Maekawa T (1995) Increase of solubility of foreign proteins in Escherichia coli by coproduction of the bacterial thioredoxin. J Biol Chem 270:25328-25331