Cost-effective expression and purification of antimicrobial and host defense peptides in Escherichia coli

Peptides

Volumn 31, Issue 11, 2010, Pages 1957-1965

  Bommarius, B ^a   Jenssen, H ^b,c   Elliott, M ^b   Kindrachuk, J ^b   Pasupuleti, Mukesh ^b   Gieren, H ^d   Jaeger, K E ^d   Hancock, R E W ^b   Kalman, D ^a  

^a EMORY UNIVERSITY   (United States)

^b UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^c ROSKILDE UNIVERSITY   (Denmark)

^d HEINRICH HEINE UNIVERSITY   (Germany)

Author keywords

Antimicrobial peptide; Bacterial expression system; Host defense peptide

Indexed keywords

ANTIMICROBIAL CATIONIC PEPTIDE; ANTIMICROBIAL PEPTIDE E5; ANTIMICROBIAL PEPTIDE E6; ANTIMICROBIAL PEPTIDE HHC10; ANTIMICROBIAL PEPTIDE IDR 1; ANTIMICROBIAL PEPTIDE MX 226; CATHELICIDIN ANTIMICROBIAL PEPTIDE LL 37; POLYPEPTIDE ANTIBIOTIC AGENT; SUMO PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANTIMICROBIAL ACTIVITY; ARTICLE; BACTERIAL STRAIN; DRUG STRUCTURE; ESCHERICHIA COLI; LARGE SCALE PRODUCTION; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; METHODOLOGY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION;

AMINO ACID SEQUENCE; ANTI-BACTERIAL AGENTS; ANTI-INFECTIVE AGENTS; ANTIMICROBIAL CATIONIC PEPTIDES; CATHELICIDINS; CLONING, MOLECULAR; COST-BENEFIT ANALYSIS; ESCHERICHIA COLI; IMMUNOLOGIC FACTORS; PEPTIDES; RECOMBINANT FUSION PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 77957840139     PISSN: 01969781     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.peptides.2010.08.008     Document Type: Article

References (46)

1. D. Andes, W. Craig, A. Nielsen, and H.H. Kristensen In vivo pharmacodynamic characterization of a novel plectasin antibiotic, NZ2114, in a murine infection model Antimicrob Agents Chemother 53 2009 3003 3009
2. B. Bommarius, and D. Kalman Antimicrobial and host defense peptides for therapeutic use against multidrug-resistant pathogens: new hope on the horizon IDrugs 12 2009 376 380
3. A.F. Bradbury, and D.G. Smyth Peptide amidation Trends Biochem Sci 16 1991 112 115
4. T.R. Butt, S.C. Edavettal, J.P. Hall, and M.R. Mattern SUMO fusion technology for difficult-to-express proteins Protein Expr Purif 43 2005 1 9
5. R. Carballar-Lejarazu, M.H. Rodriguez, F. de la Cruz Hernandez-Hernandez, J. Ramos-Castaneda, L.D. Possani, and M. Zurita-Ortega Recombinant scorpine: a multifunctional antimicrobial peptide with activity against different pathogens Cell Mol Life Sci 65 2008 3081 3092
6. M. Carretero, M.J. Escamez, M. Garcia, B. Duarte, A. Holguin, and L. Retamosa In vitro and in vivo wound healing-promoting activities of human cathelicidin LL-37 J Invest Dermatol 128 2008 223 236
7. X. Chen, F. Zhu, Y. Cao, and S. Qiao Novel expression vector for secretion of cecropin AD in Bacillus subtilis with enhanced antimicrobial activity Antimicrob Agents Chemother 53 2009 3683 3689
8. Y.Q. Chen, S.Q. Zhang, B.C. Li, W. Qiu, B. Jiao, and J. Zhang Expression of a cytotoxic cationic antibacterial peptide in Escherichia coli using two fusion partners Protein Expr Purif 57 2008 303 311
9. A. Cherkasov, K. Hilpert, H. Jenssen, C.D. Fjell, M. Waldbrook, and S.C. Mullaly Use of artificial intelligence in the design of small peptide antibiotics effective against a broad spectrum of highly antibiotic-resistant superbugs ACS Chem Biol 4 2009 65 74
10. T.A. Czyzyk, Y. Ning, M.S. Hsu, B. Peng, R.E. Mains, and B.A. Eipper Deletion of peptide amidation enzymatic activity leads to edema and embryonic lethality in the mouse Dev Biol 287 2005 301 313
11. M. Gilliet, and R. Lande Antimicrobial peptides and self-DNA in autoimmune skin inflammation Curr Opin Immunol 20 2008 401 407
12. P. Hamill, K. Brown, H. Jenssen, and R.E. Hancock Novel anti-infectives: is host defence the answer? Curr Opin Biotechnol 19 2008 628 636
13. R.E. Hancock, and H.G. Sahl Antimicrobial and host-defense peptides as new anti-infective therapeutic strategies Nat Biotechnol 24 2006 1551 1557
14. C. Haught, G.D. Davis, R. Subramanian, K.W. Jackson, and R.G. Harrison Recombinant production and purification of novel antisense antimicrobial peptide in Escherichia coli Biotechnol Bioeng 57 1998 55 61
15. K. Hilpert, R. Volkmer-Engert, T. Walter, and R.E. Hancock High-throughput generation of small antibacterial peptides with improved activity Nat Biotechnol 23 2005 1008 1012
16. I. Hong, Y.S. Kim, and S.G. Choi Simple purification of human antimicrobial peptide dermcidin (MDCD-1L) by intein-mediated expression in E. coli J Microbiol Biotechnol 20 2010 350 355
17. K.H. Hsu, C. Pei, J.Y. Yeh, C.H. Shih, Y.C. Chung, and L.T. Hung Production of bioactive human alpha-defensin 5 in Pichia pastoris J Gen Appl Microbiol 55 2009 395 401
18. H. Jenssen, C.D. Fjell, A. Cherkasov, and R.E. Hancock QSAR modeling and computer-aided design of antimicrobial peptides J Pept Sci 14 2008 110 114
19. H. Jenssen, P. Hamill, and R.E. Hancock Peptide antimicrobial agents Clin Microbiol Rev 19 2006 491 511
20. H. Jenssen, and R.E. Hancock Therapeutic potential of HDPs as immunomodulatory agents Methods Mol Biol 618 2010 329 347
21. H. Jenssen, T. Lejon, K. Hilpert, C.D. Fjell, A. Cherkasov, and R.E. Hancock Evaluating different descriptors for model design of antimicrobial peptides with enhanced activity toward P. aeruginosa Chem Biol Drug Des 70 2007 134 142
22. T. Jin, M. Bokarewa, T. Foster, J. Mitchell, J. Higgins, and A. Tarkowski Staphylococcus aureus resists human defensins by production of staphylokinase, a novel bacterial evasion mechanism J Immunol 172 2004 1169 1176
23. J.M. Kim, S.A. Jang, B.J. Yu, B.H. Sung, J.H. Cho, and S.C. Kim High-level expression of an antimicrobial peptide histonin as a natural form by multimerization and furin-mediated cleavage Appl Microbiol Biotechnol 78 2008 123 130
24. D.J. Korz, U. Rinas, K. Hellmuth, E.A. Sanders, and W.D. Deckwer Simple fed-batch technique for high cell density cultivation of Escherichia coli J Biotechnol 39 1995 59 65
25. J.F. Li, J. Zhang, R. Song, J.X. Zhang, Y. Shen, and S.Q. Zhang Production of a cytotoxic cationic antibacterial peptide in Escherichia coli using SUMO fusion partner Appl Microbiol Biotechnol 84 2009 383 388
26. Y. Li Carrier proteins for fusion expression of antimicrobial peptides in Escherichia coli Biotechnol Appl Biochem 54 2009 1 9
27. M.P. Malakhov, M.R. Mattern, O.A. Malakhova, M. Drinker, S.D. Weeks, and T.R. Butt SUMO fusions and SUMO-specific protease for efficient expression and purification of proteins J Struct Funct Genomics 5 2004 75 86
28. N. Mookherjee, and R.E. Hancock Cationic host defence peptides: innate immune regulatory peptides as a novel approach for treating infections Cell Mol Life Sci 64 2007 922 933
29. N. Mookherjee, H.L. Wilson, S. Doria, Y. Popowych, R. Falsafi, and J.J. Yu Bovine and human cathelicidin cationic host defense peptides similarly suppress transcriptional responses to bacterial lipopolysaccharide J Leukoc Biol 80 2006 1563 1574
30. P.H. Mygind, R.L. Fischer, K.M. Schnorr, M.T. Hansen, C.P. Sonksen, and S. Ludvigsen Plectasin is a peptide antibiotic with therapeutic potential from a saprophytic fungus Nature 437 2005 975 980
31. K.L. Piers, M.H. Brown, and R.E. Hancock Recombinant DNA procedures for producing small antimicrobial cationic peptides in bacteria Gene 134 1993 7 13
32. M. Pranting, A. Negrea, M. Rhen, and D.I. Andersson Mechanism and fitness costs of PR-39 resistance in Salmonella enterica serovar Typhimurium LT2 Antimicrob Agents Chemother 52 2008 2734 2741
33. A. Pripp, T. Isaksson, L. Stepaniak, T. Sorhaug, and Y. Ardo Quantitative structure activity relationship modelling of peptides and proteins as a tool in food science Trends Food Sci Technol 16 2005 484 494
34. D. Reverter, and C.D. Lima A basis for SUMO protease specificity provided by analysis of human Senp2 and a Senp2-SUMO complex Structure 12 2004 1519 1531
35. H. Schagger, and G. von Jagow Tricine-sodium dodecyl sulfate- polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa Anal Biochem 166 1987 368 379
36. M.G. Scott, E. Dullaghan, N. Mookherjee, N. Glavas, M. Waldbrook, and A. Thompson An anti-infective peptide that selectively modulates the innate immune response Nat Biotechnol 25 2007 465 472
37. B. Spellberg, R. Guidos, D. Gilbert, J. Bradley, H.W. Boucher, and W.M. Scheld The epidemic of antibiotic-resistant infections: a call to action for the medical community from the Infectious Diseases Society of America Clin Infect Dis 46 2008 155 164
38. P. Tadas, C. Sanders, and T.R. Butt SUMO fusion technology for enhanced protein production in prokaryotic and eukaryotic expression systems. SUMO protocols 2009 Humana Press New York pp. 303-17
39. V.I. Tishkov, A.G. Galkin, V.V. Fedorchuk, P.A. Savitsky, A.M. Rojkova, and H. Gieren Pilot scale production and isolation of recombinant NAD+- and NADP+-specific formate dehydrogenases Biotechnol Bioeng 64 1999 187 193
40. I. Wiegand, K. Hilpert, and R.E. Hancock Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances Nat Protoc 3 2008 163 175
41. M.R. Yeaman, and N.Y. Yount Mechanisms of antimicrobial peptide action and resistance Pharmacol Rev 55 2003 27 55
42. J. Yu, N. Mookherjee, K. Wee, D.M. Bowdish, J. Pistolic, and Y. Li Host defense peptide LL-37, in synergy with inflammatory mediator IL-1beta, augments immune responses by multiple pathways J Immunol 179 2007 7684 7691
43. M. Zasloff Antimicrobial peptides of multicellular organisms Nature 415 2002 389 395
44. L. Zhang, T. Falla, M. Wu, S. Fidai, J. Burian, and W. Kay Determinants of recombinant production of antimicrobial cationic peptides and creation of peptide variants in bacteria Biochem Biophys Res Commun 247 1998 674 680
45. Q.F. Zhou, X.G. Luo, L. Ye, and T. Xi High-level production of a novel antimicrobial peptide perinerin in Escherichia coli by fusion expression Curr Microbiol 54 2007 366 370
46. M. Zorko, B. Japelj, I. Hafner-Bratkovic, and R. Jerala Expression, purification and structural studies of a short antimicrobial peptide Biochim Biophys Acta 1788 2009 314 323