A novel protocol for the production of recombinant LL-37 expressed as a thioredoxin fusion protein

Protein Expression and Purification

Volumn 81, Issue 2, 2012, Pages 201-210

  Li, Yifeng ^a  

^a UNIVERSITY OF TEXAS HEALTH SCIENCE CENTER AT SAN ANTONIO   (United States)

Author keywords

Antimicrobial peptide; LL 37; Oligomerization; Thioredoxin fusion protein; Thrombin cleavage

Indexed keywords

ANTIINFECTIVE AGENT; CATHELICIDIN; CATHELICIDIN ANTIMICROBIAL PEPTIDE; HYBRID PROTEIN; THIOREDOXIN; THROMBIN;

AMINO ACID SEQUENCE; ARTICLE; BIOSYNTHESIS; CHEMISTRY; ENZYME ACTIVE SITE; ESCHERICHIA COLI; GEL CHROMATOGRAPHY; GENE VECTOR; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; MICROBIOLOGICAL EXAMINATION; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; SOLUBILITY;

AMINO ACID SEQUENCE; ANTI-BACTERIAL AGENTS; BASE SEQUENCE; CATALYTIC DOMAIN; CATHELICIDINS; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENETIC VECTORS; MICROBIAL SENSITIVITY TESTS; MOLECULAR SEQUENCE DATA; PLASMIDS; RECOMBINANT FUSION PROTEINS; SOLUBILITY; THIOREDOXINS; THROMBIN;

ANIMALIA; ESCHERICHIA COLI;

EID: 82155186247     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2011.10.011     Document Type: Article

References (28)

1. O.E. Sørensen, P. Follin, A.H. Johnsen, J. Calafat, G.S. Tjabringa, P.S. Hiemstra, and N. Borregaard Human cathelicidin, hCAP-18, is processed to the antimicrobial peptide LL-37 by extracellular cleavage with proteinase 3 Blood 97 2001 3951 3959
2. U.H. Dürr, U.S. Sudheendra, and A. Ramamoorthy LL-37, the only human member of the cathelicidin family of antimicrobial peptides Biochim. Biophys. Acta 1758 2006 1408 1425 (Pubitemid 44436081)
3. W.K. Wu, G. Wang, S.B. Coffelt, A.M. Betancourt, C.W. Lee, D. Fan, K. Wu, J. Yu, J.J. Sung, and C.H. Cho Emerging roles of the host defense peptide LL-37 in human cancer and its potential therapeutic applications Int. J. Cancer 127 2010 1741 1747
4. A. Nijnik, and R.E. Hancock The roles of cathelicidin LL-37 in immune defences and novel clinical applications Curr. Opin. Hematol. 16 2009 41 47
5. P. Méndez-Samperio The human cathelicidin hCAP18/LL-37: a multifunctional peptide involved in mycobacterial infections Peptides 31 2010 1791 1798
6. R. Bucki, K. Leszczyńska, A. Namiot, and W. Sokołowski Cathelicidin LL-37, A multitask antimicrobial peptide Arch. Immunol. Ther. Exp. (Warsz) 58 2011 15 25
7. J.A. Hensel, D. Chanda, S. Kumar, A. Sawant, W.E. Grizzle, G.P. Siegal, and S. Ponnazhagan LL-37 as a therapeutic target for late stage prostate cancer Prostate 71 2011 659 670
8. T. Leung, K. Ching, A. Kong, G. Wong, J. Chan, K. Hon, Circulating LL-37 is a biomarker for eczema severity in children, J. Eur. Acad. Dermatol. Venereol. (in press), doi: 10.1111/j.1468-3083.2011.04083.x.
9. A.B. Ingham, and R.J. Moore Recombinant production of antimicrobial peptides in heterologous microbial systems Biotechnol. Appl. Biochem. 47 2007 1 9 (Pubitemid 46762266)
10. Y. Li, and Z. Chen RAPD: a database of recombinantly-produced antimicrobial peptides FEMS Microbiol. Lett. 289 2008 126 129
11. Y. Li Recombinant production of antimicrobial peptides in Escherichia coli: A review Protein Expr. Purif. 80 2011 260 267
12. Y. Li Carrier proteins for fusion expression of antimicrobial peptides in Escherichia coli Biotechnol. Appl. Biochem. 54 2009 1 9
13. Y. Feng, Y. Yang, N. Huang, Q. Wu, and B. Wang E. coli-based production of recombinant FALL-39 Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 20 2003 634 637
14. J.Y. Moon, K.A. Henzler-Wildman, and A. Ramamoorthy Expression and purification of a recombinant LL-37 from Escherichia coli Biochim. Biophys. Acta 1758 2006 1351 1358 (Pubitemid 44436070)
15. Y.H. Yang, G.G. Zheng, G. Li, X.J. Zhang, Z.Y. Cao, Q. Rao, and K.F. Wu Expression of bioactive recombinant GSLL-39, a variant of human antimicrobial peptide LL-37, in Escherichia coli Protein Expr. Purif. 37 2004 229 235 (Pubitemid 39027265)
16. Y. Li, X. Li, and G. Wang Cloning, expression, isotope labeling, and purification of human antimicrobial peptide LL-37 in Escherichia coli for NMR studies Protein Expr. Purif. 47 2006 498 505 (Pubitemid 43744452)
17. Y. Li, X. Li, H. Li, O. Lockridge, and G. Wang A novel method for purifying recombinant human host defense cathelicidin LL-37 by utilizing its inherent property of aggregation Protein Expr. Purif. 54 2007 157 165 (Pubitemid 46636313)
18. Y. Li, Expression Systems and Database for Biological, Structural and Bioinformatic Studies of Antimicrobial Peptides. Ph.D. Dissertation, University of Nebraska Medical Center, Omaha, NE. (UMI No. 3314590), 2008.
19. J. Krahulec, M. Hyršová, S. Pepeliaev, J. Jílková, Z. Cerný, and J. Machálková High level expression and purification of antimicrobial human cathelicidin LL-37 in Escherichia coli Appl. Microbiol. Biotechnol. 88 2010 167 175
20. R. Ramos, L. Domingues, and M. Gama Escherichia coli expression and purification of LL37 fused to a family III carbohydrate-binding module from Clostridium thermocellum Protein Expr. Purif. 71 2010 1 7
21. B. Bommarius, H. Jenssen, M. Elliott, J. Kindrachuk, M. Pasupuleti, H. Gieren, K.E. Jaeger, R.E. Hancock, and D. Kalman Cost-effective expression and purification of antimicrobial and host defense peptides in Escherichia coli Peptides 31 2010 1957 1965
22. J. Johansson, G.H. Gudmundsson, M.E. Rottenberg, K.D. Berndt, and B. Agerberth Conformation-dependent antibacterial activity of the naturally occurring human peptide LL-37 J. Biol. Chem. 273 1998 3718 3724 (Pubitemid 28109800)
23. R.A. Dorschner, V.K. Pestonjamasp, S. Tamakuwala, T. Ohtake, J. Rudisill, V. Nizet, B. Agerberth, G.H. Gudmundsson, and R.L. Gallo Cutaneous injury induces the release of cathelicidin anti-microbial peptides active against group A Streptococcus J. Invest. Dermatol. 117 2001 91 97 (Pubitemid 32652444)
24. K.L. Piers, M.H. Brown, and R.E. Hancock Recombinant DNA procedures for producing small antimicrobial cationic peptides in bacteria Gene 134 1993 7 13
25. G.M. Gibbs, B.E. Davidson, and A.J. Hillier Novel expression system for large-scale production and purification of recombinant class IIa bacteriocins and its application to piscicolin 126 Appl. Environ. Microbiol. 70 2004 3292 3297 (Pubitemid 38745870)
26. L. Beaulieu, D. Tolkatchev, J.F. Jetté, D. Groleau, and M. Subirade Production of active pediocin PA-1 in Escherichia coli using a thioredoxin gene fusion expression approach: cloning, expression, purification, and characterization Can. J. Microbiol. 53 2007 1246 1258
27. X.J. Feng, J.H. Wang, A.S. Shan, D. Teng, Y.L. Yang, Y. Yao, G.P. Yang, Y.C. Shao, S. Liu, and F. Zhang Fusion expression of bovine lactoferricin in Escherichia coli Protein Expr. Purif. 47 2006 110 117
28. Q.F. Zhou, X.G. Luo, L. Ye, and T. Xi High-level production of a novel antimicrobial peptide perinerin in Escherichia coli by fusion expression Curr. Microbiol. 54 2007 366 370 (Pubitemid 46733871)