Highly efficient production of the Alzheimer's γ-Secretase integral membrane protease complex by a multi-gene stable integration approach

Biotechnology and Bioengineering

Volumn 110, Issue 7, 2013, Pages 1995-2005

  Alattia, Jean René ^a   Matasci, Mattia ^a   Dimitrov, Mitko ^a   Aeschbach, Lorène ^a   Balasubramanian, Sowmya ^a   Hacker, David L ^a   Wurm, Florian M ^a   Fraering, Patrick C ^a  

^a EPFL   (Switzerland)

Author keywords

secretase; Alzheimer's disease; Membrane embedded multi protein complexes; Protein expression and purification; Transposon mediated multigene stable integration into genomic DNA

Indexed keywords

ALZHEIMER'S DISEASE; GENOMIC DNA; MULTI-PROTEIN COMPLEX; PROTEIN EXPRESSIONS; SECRETASES;

DRUG PRODUCTS; GENES; INTEGRATION; TRANSCRIPTION;

PROTEINS;

GAMMA SECRETASE; PROTEASOME;

ALZHEIMER DISEASE; ANIMAL CELL; ARTICLE; CATALYSIS; CHO CELL; CONTROLLED STUDY; CRYSTALLIZATION; DHFR GENE; EXPRESSION VECTOR; GENE; GENE DOSAGE; GENE TRANSFER; MOLECULAR BIOLOGY; NONHUMAN; PAC GENE; PROTEIN DEGRADATION; PROTEIN SYNTHESIS; TRANSPOSON;

AMYLOID PRECURSOR PROTEIN SECRETASES; ANIMALS; BIOTECHNOLOGY; CHO CELLS; CRICETULUS; DNA TRANSPOSABLE ELEMENTS; GENETIC VECTORS; METABOLIC ENGINEERING; MULTIPROTEIN COMPLEXES; RECOMBINANT PROTEINS; RECOMBINATION, GENETIC;

MAMMALIA;

EID: 84878382150     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24851     Document Type: Article

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