Detection of viable but nonculturable Escherichia coli O157:H7 using propidium monoazide treatments and qPCR

Canadian Journal of Microbiology

Volumn 59, Issue 3, 2013, Pages 157-163

  Xiao, Xing Long ^a   Tian, Cong ^a   Yu, Yi Gang ^a   Wu, Hui ^a  

^a SOUTH CHINA UNIVERSITY OF TECHNOLOGY   (China)

Author keywords

Detection; Escherichia coli O157:H7; Propidium monoazide; qPCR; Viable but not culturable (VBNC)

Indexed keywords

PROPIDIUM IODIDE;

ACCURACY ASSESSMENT; DISEASE TREATMENT; ENVIRONMENTAL FACTOR; MICROCOSM; NUMERICAL MODEL; NUTRITIONAL STATUS; POLYMERASE CHAIN REACTION; SALINITY; SENSITIVITY ANALYSIS;

ARTICLE; BACTERIAL SURVIVAL; BACTERIAL VIABILITY; BACTERIUM DETECTION; COLONY FORMING UNIT; CROSS LINKING; ENVIRONMENTAL FACTOR; ESCHERICHIA COLI O157; IN VITRO STUDY; LIMIT OF DETECTION; LIMIT OF QUANTITATION; LOW TEMPERATURE; MICROCOSM; NONHUMAN; NUTRITIONAL STATUS; PRIORITY JOURNAL; YEAST;

AZIDES; BACTERIAL LOAD; CULTURE MEDIA; DNA, BACTERIAL; ESCHERICHIA COLI O157; PHENANTHRIDINES; PROPIDIUM; REAL-TIME POLYMERASE CHAIN REACTION;

ESCHERICHIA COLI; LURIA;

EID: 84875647775     PISSN: 00084166     EISSN: 14803275     Source Type: Journal    
DOI: 10.1139/cjm-2012-0577     Document Type: Article

References (36)

1. Alan, B., and James, D.O. 2010. Survival of spinach-associated Helicobacter pylori in the viable but nonculturable state. Food Control, 21: 1150-1154. doi:10.1016/j. foodcont.2010.01.010.
2. Andreas, N., Priscilla, S.F., Mark, D.B., and Anne, K.C. 2007. Use of propidium monoazide for live/dead distinction in microbial ecology. Appl. Environ. Microbiol. 8: 5111-5117. PMID:17586667.
3. Arana, I., Muela, A., Orruno, M., Seco, C., Garaizabal, I., and Barcina, I. 2010. Effect of temperature and starvation upon survival strategies of Pseudomonas fluorescens CHA0: comparison with Escherichia coli. FEMS Microbiol. Ecol. 74: 500-509. doi:10.1111/j.1574-6941.2010.00979.x. PMID:20955194.
4. Bae, S., and Wuertz, S. 2009. Rapid decay of host-specific fecal Bacteroidales cells in seawater as measured by quantitative PCR with propidium monoazide. Water Res. 43(19): 4850-4859. doi:10.1016/j.watres.2009.06.053. PMID: 19656546.
5. Carol, A.P. 1999. The epidemiology, detection and control of Escherichia coli O157. J. Sci. Food Agric. 79: 1367-1381. doi:10.1002/(SICI)1097-0010(199908)79: 11<1367::AID-JSFA374>3.0.CO;2-S.
6. Chalmers, R.M., Aird, H., and Bolton, F.J. 2000. Waterborne Escherichia coli O157: H7. J. Appl. Microbiol. 88: 124S-132S. doi:10.1111/j.1365-2672.2000.tb05340.x.
7. Duodu, S., and Colquhoun, D. 2010. Monitoring the survival of fish-pathogenic Francisella in water microcosms. FEMS Microbiol. Ecol. 74(3): 534-541. doi:10. 1111/j.1574-6941.2010.00973.x. PMID:20977492.
8. Elizaquivel, P., Sanchez, G., Selma, M.M., and Aznar, R. 2012. Application of propidium monoazide-qPCR to evaluate the ultrasonic inactivation of Escherichia coli O157:H7 in fresh-cut vegetable wash water. Food Microbiol. 30: 316-320. doi:10.1016/j.fm.2011.10.008. PMID:22265318.
9. Enrica, O., Giulia, A., Giorgio, B., and Mauro, M. 2009. A new platform for real-time PCR detection of Salmonella spp., Listeria monocytogenes and Escherichia coli O157 in milk. Food Microbiol. 26: 615-622. doi:10.1016/j.fm.2009.04. 008. PMID:19527837.
10. Garcia-Armisen, T., and Servais, P. 2004. Enumeration of viable E. coli in rivers and wastewaters by fluorescent in situ hybridization. J. Microbiol. Methods, 58(2): 269-279. doi:10.1016/j.mimet.2004.04.014. PMID:15234525.
11. Higgins, M.J., Chen, Y.-C., Murthy, S.N., Hendrickson, D., Farrel, J., and Schafer, P. 2007. Reactivation and growth of non-culturable indicator bacteria in anaerobically digested biosolids after centrifuge dewatering. Water Res. 41: 665-673. doi:10.1016/j.watres.2006.09.017. PMID:17107701.
12. Kogure, K., Simidu, U., and Taga, N. 1978. A tentative direct microscopic method for counting living marine bacteria. Can. J. Microbiol. 25: 415-420. doi:10. 1139/m79-063. PMID:378340.
13. Li, B., and Chen, J.Q. 2012. Real-time PCR methodology for selective detection of viable Escherichia coli O157:H7 by targeting Z3276 as a genetic marker. Appl. Environ. Microbiol. 78: 5297-5304. doi:10.1128/AEM.00794-12. PMID: 22635992.
14. Liu, Y., Gilchrist, A., Zhang, J., and Li, X.F. 2008. Detection of viable but nonculturable Escherichia coli O157:H7 bacteria in drinking water and river water. Appl. Environ. Microbiol. 74: 1502-1507. doi:10.1128/AEM.02125-07. PMID: 18203853.
15. Liu, Y., Wang, C., Fung, C., and Li, X.-F. 2010. Quantification of viable but nonculturable Escherichia coli O157:H7 by targeting the rpoS mRNA. Anal. Chem. 82(7): 2612-2615. doi:10.1021/ac1003272. PMID:20230052.
16. Marouani-Gadri, N., Firmesse, O., Chassaing, D., Sandris-Nielsen, D., Arneborg, N., and Carpentier, B. 2010. Potential of Escherichia coli O157:H7 to persist and form viable but non-culturable cells on a food-contact surface subjected to cycles of soiling and chemical treatment. Int. J. Food Microbiol. 144(1): 96-103. doi:10.1016/j.ijfoodmicro.2010.09.002. PMID:20888655.
17. Mizunoe, Y., Wai, S.N., Takade, A., and Yoshida, S. 1999. Restoration of culturability of starvation-stressed and low-temperature-stressed Escherichia coli O157 cells by using H2O2-degrading compounds. Arch. Microbiol. 172: 63-67. doi:10.1007/s002030050741. PMID:10398754.
18. Nocker, A., and Camper, A.K. 2006. Selective removal of DNA from dead cells of mixed bacterial communities by use of ethidium monoazide. Appl. Environ. Microbiol, 72: 1997-2004. doi:10.1128/AEM.72.3.1997-2004.2006. PMID: 16517648.
19. Nocker, A., Cheung, C.Y., and Camper, A.K. 2006. Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cell. J. Microbiol. Methods, 67: 310-320. doi:10.1016/j.mimet.2006.04.015. PMID:16753236.
20. Nogva, H., Dromtorp, S., Nissen, H., and Rudi, K. 2003. Ethidium monoazide for DNA-based differentiation of viable and dead bacteria by 5=-nuclease PCR. BioTechniques, 34: 804-813. PMID:12703305.
21. Oliver, J.D. 2005. The viable but nonculturable state in bacteria. J. Microbiol. 43: 93-100. PMID:15765062.
22. Oliver, J.D. 2010. Recent findings on the viable but nonculturable state in pathogenic bacteria. FEMS Microbiol. Rev. 34: 415-425. doi:10.1111/j.1574-6976.2009. 00200.x. PMID:20059548.
23. Pawlowski, D.R., Metzger, D.J., Raslawsky, A., Howlett, A., Siebert, G., Karalus, R.J., et al. 2011. Entry of Yersinia pestis into the viable but nonculturable state in a low-temperature tap water microcosm. PLoS One, 6: e17585. doi:10.1371/journal.pone.0017585. PMID:21436885.
24. Phillip, B.G., and Betty, H.O. 2009. Development of a quantitative PCR method to differentiate between viable and nonviable bacteria in environmental water samples. Appl. Microbiol. Biotechnol. 82: 587-596. doi:10.1007/s00253-008-1846-y. PMID:19153730.
25. Raymond, L., Kepner, J.R., and James, R.P. 1994. Use of fluorochromes for direct enumeration of total bacteria in environmental samples: past and present. Microbiol. Mol. Biol. Rev. 58: 603-615.
26. Reissbrodt, R., Rienaecker, I., Romanova, J.M., Freestone, P.P.E., Haigh, R.D., Lyte, M., et al. 2002. Resuscitation of Salmonella enterica serovar Typhimurium and enterohemorrhagic Escherichia coli from the viable but nonculturable state by heat stable enterobacterial autoinducer. Appl. Environ. Microbiol. 68(10): 4788-4794. doi:10.1128/AEM.68.10.4788-4794.2002. PMID:12324321.
27. Rigsbee, W., Simpson, L.M., and Oliver, J.D. 1997. Detection of the viable but nonculturable state in Escherichia coli O157:H7. Journal of Food Safety, 16(4): 255-262. doi:10.1111/j.1745-4565.1997.tb00166.x.
28. Rowan, N.J. 2004. Viable but nonculturable forms of food and waterborne bacteria: QuoVadis? Trends Food Sci. Technol. 15: 462-467. doi:10.1016/j.tifs.2004. 02.009.
29. Rudi, K., Naterstad, K., Dromtorp, S.M., and Holo, H. 2009. Detection of viable and dead Listeria monocytogenes on gouda-like cheeses by real-time PCR. Lett. Appl. Microbiol. 40: 301-306. doi:10.1111/j.1472-765X.2005.01672.x. PMID: 15752222.
30. Sheridan, G.E.C., Masters, C.I., Shallcross, J.A., and Mackey, B.M. 1998. Detection of mRNA by reverse transcription-PCR as an indicator of viability in Escherichia coli Cells. Appl. Environ. Microbiol. 64: 1313-1318. PMID:9546166.
31. Sun, F., Chen, J., Zhong, L., Zhang, X.-H., Wang, R., Guo, Q., and Dong, Y. 2008. Characterization and virulence retention of viable but nonculturable Vibrio harveyi. FEMS Microbiol. Ecol. 64: 37-44. doi:10.1111/j.1574-6941.2008.00442.x. PMID:18248440.
32. Varma, M., Field, R., Stinson, M., Rukovets, B., Wymer, L., and Haugland, R. 2009. Quantitative real-time PCR analysis of total and propidium monoazideresistant fecal indicator bacteria in wastewater. Water Res. 43: 4790-4801. doi:10.1016/j.watres.2009.05.031. PMID:19540546.
33. Vesper, S., McKinstry, C., Hartmann, C., Neace, M., Yoder, S., and Vesper, A. 2008. Quantifying fungal viability in air and water samples using quantitative PCR after treatment with propidium monoazide (PMA). J. Microbiol. Methods, 72(2): 180-184. doi:10.1016/j.mimet.2007.11.017. PMID:18160156.
34. Wang, Y., Claeys, L., van der Ha, D., Verstraete, W., and Boon, N. 2010. Effects of chemically and electrochemically dosed chlorine on Escherichia coli and Legionella beliardensis assessed by flow cytometry. Appl. Microbiol. Biotechnol. 87(1): 331-341. doi:10.1007/s00253-010-2526-2. PMID:20352423.
35. Xu, H.-S., Roberts, N., Singleton, F.L., Attwell, R.W., Grimes, D.J., and Colwell, R.R. 1982. Survival and viability of non-culturable Escherichia coli and Vibrio cholerae in the estuarine and marine environment. Microb. Ecol. 8(4): 313-323. doi:10.1007/BF02010671.
36. Yang, X., Badoni, M., and Gill, C.O. 2011. Use of propidium monoazide and quantitative PCR for differentiation of viable Escherichia coli from E. coli killed by mild or pasteurizing heat treatments. Food Microbiol. 28(8): 1478-1482. doi:10.1016/j.fm.2011.08.013. PMID:21925032.