Development of a quantitative PCR method to differentiate between viable and nonviable bacteria in environmental water samples

Applied Microbiology and Biotechnology

Volumn 82, Issue 3, 2009, Pages 587-596

  Gedalanga, Phillip B ^a   Olson, Betty H ^b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b University of California at Irvine   (United States)

Author keywords

Cell viability; Environmental and wastewaters; Escherichia coli; Ethidium monoazide bromide; QPCR

Indexed keywords

ACTIVATED SLUDGE EFFLUENTS; BACTERIAL CULTURES; CELL VIABILITY; E. COLI; ENVIRONMENTAL AND WASTEWATERS; ENVIRONMENTAL SAMPLES; ENVIRONMENTAL WATER SAMPLES; ENVIRONMENTAL WATERS; ETHIDIUM MONOAZIDE BROMIDE; LOW CONCENTRATIONS; OPTIMUM CONDITIONS; PRIMARY CLARIFIERS; PURE CULTURES; QPCR; QUANTITATIVE PCR; QUANTITATIVE POLYMERASE CHAIN REACTIONS; SAMPLE MATRICES; TRICKLING FILTERS; TURBIDITY VALUES; VIABLE BUT NON-CULTURABLE;

AMPLIFICATION; ASSAYS; BACTERIOLOGY; CLARIFICATION; EFFLUENT TREATMENT; EFFLUENTS; ESCHERICHIA COLI; GENETIC ENGINEERING; PUMPS; TURBIDITY; WASTEWATER; WASTEWATER TREATMENT; WATER RECYCLING;

CELL CULTURE;

ACTIVATED SLUDGE; BACTERIUM; BIOASSAY; FILTER; GENE EXPRESSION; ORGANIC NITROGEN COMPOUND; POLYMERASE CHAIN REACTION; TURBIDITY; VIABILITY; WASTEWATER; WATER TREATMENT;

AZIDES; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FRESH WATER; MICROBIAL VIABILITY; POLYMERASE CHAIN REACTION; SEWAGE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 60549096842     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-008-1846-y     Document Type: Article

References (31)

1. J Bernhardt J Weibezahn C Scharf M Hecker 2003 Bacillus subtilis during feast and famine: visualization of the overall regulation of protein synthesis during glucose starvation by proteome analysis Genome Res 13 2 224 237
2. Boone AM (2007) Rapid detection techniques useful for primary indicators of activated sludge treatment optimization. Master's Thesis-Spring 2007. John Hopkins University
3. RE Cantwell R Hofmann 2008 Inactivation of indigenous coliform bacteria in unfiltered surface water by ultraviolet light Water Res 42 2729 2735
4. C Condon CL Squires 1995 Control of rRNA transcription in Escherichia coli Microbiol Rev 59 4 623 645
5. E Dupray MP Caprais A Derrien P Fach 1997 Salmonella DNA persistence in natural seawaters using PCR analysis J Appl Microbiol 82 4 507 510
6. G Flekna S Polonca M Wagner FJM Smulders SS Mozina I Hein 2007 Insufficient differentiation of live and dead Campylobacter jejuni and Listeria monocytogenes cells by ethidium monoazide (EMA) compromises EMA/real-time PCR Res Microbiol 158 405 412
7. RA Guy A Kapoor J Holicka D Shepherd PA Horgen 2006 A rapid molecular-based assay for direct quantification of viable bacteria in slaughterhouses J Food Protection 69 6 1265 1272
8. JW He SC Jiang 2005 Quantification of enterococci and human adenoviruses in environmental samples by real-time PCR Appl Environ Microbiol 71 5 2250 2255
9. L He J Lu W Shi 2007 Variability of fecal indicator bacteria in flowing and ponded waters in southern California: implications for bacterial TMDL development and implementation Water Res 41 3132 3140
10. HC Jeng AJ England HB Bradford 2005 Indicator organisms associated with stormwater suspended particles and estuarine sediment J Environ Sci Health 40 779 791
11. KL Josephson CP Gerba IL Pepper 1993 Polymerase chain reaction detection of nonviable bacterial pathogens Appl Environ Microbiol 59 10 3513 3515
12. 90 values for modeling faecal indicators in recreational waters Water Res 39 655 667
13. MW LeChevallier TM Evans RJ Seidler 1981 Effect of turbidity on chlorination efficiency and bacterial persistence in drinking water Appl Environ Microbiol 42 1 159 167
14. JL Lee RE Levin 2006 Use of ethidium bromide monoazide for quantification of viable and dead mixed bacterial flora from fish fillets by polymerase chain reaction J Microbiol Methods 67 456 462
15. CI Masters CA Miles BM Mackey 1994 Effect of stress treatments on the detection of Listeria monocytogenes and enterotoxigenic Escherichia coli by the polymerase chain reaction J Appl Bacteriol 77 1 73 79
16. K Matsuda H Tsuji T Asahara Y Kado K Nomoto 2007 Sensitive quantitative detection of commensal bacteria by rRNA-targeted reverse transcription-PCR Appl Environ Microbiol 73 1 32 39
17. P McGee DJ Bolton JJ Sheridan B Earley G Kelly N Leonard 2002 Survival of Escherichia coli O157:H7 in farm water: its role as a vector in the transmission of the organism within herds J Appl Microbiol 93 4 706 713
18. RG Mirpuri WL Jones GA McFeters HF Ridgway 1997 Physiological stress in batch cultures of Pseudomonas putida 54G during toluene degradation J Ind Microbiol Biotechnol 18 6 406 413
19. AN Nocker AK Camper 2006 Selective removal of DNA from dead cells of mixed bacterial communities by use of ethidium monoazide Appl Environ Microbiol 72 3 1997 2004
20. AN Nocker CY Cheung AK Camper 2006 Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cells J Microbiol Methods 67 310 320
21. HK Nogva SM Dromtorp H Nissen K Rudi 2003 Ethidium monoazide for DNA-based differentiation of viable and dead bacteria by 5′-nuclease PCR BioTechniques 34 804 813
22. DM Norton CA Batt 1999 Detection of viable Listeria monocytogenes with a 5′ nuclease PCR assay Appl Environ Microbiol 65 5 2122 2127
23. HF Ridgway BH Olson 1981 Chlorine resistance patterns of bacteria from two drinking water distribution systems Appl Environ Microbiol 44 4 972 987
24. K Rudi B Moen SM Dromtorp AL Holck 2005 Use of ethidium monoazide and PCR in combination for quantification of viable and dead cells in complex samples Appl Environ Microbiol 71 2 1018 1024
25. K Rudi K Naterstad SM Dromtorp H Holo 2005 Detection of viable and dead Listeria monocytogenes on gouda-like cheeses by real-time PCR Lett Appl Microbiol 40 301 306
26. GEC Sheridan CI Masters JA Shallcross BM Mackey 1998 Detection of mRNA by reverse transcription-PCR as an indicator of viability in Escherichia coli cells Appl Environ Microbiol 64 4 1313 1318
27. MH Stewart BH Olson l992 Physiological studies of chloramine resistance developed by Klebsiella pneumoniae under low nutrient growth conditions Appl Environ Microbiol 58 9 2918 2927
28. Stewart MH, Olson B (1996) Bacterial resistance to potable water disinfectants. In: Hurst CJ (ed) Modeling disease transmission and its prevention by disinfection. Cambridge University Press, pp 140-192
29. S Wang RE Levin 2005 Discrimination of viable Vibrio vulnificus cells from dead cells in real-time PCR J Microbiol Methods 64 1 8
30. S Yaron KR Matthews 2002 A reverse transcriptase-polymerase chain reaction assay for detection of viable Escherichia coli O157:H7: investigation of specific target genes J Appl Microbiol 92 633 640
31. Z Yu WW Mohn 1999 Killing two birds with one stone: simultaneous extraction of DNA and RNA from activated sludge biomass Can J Microbiol 45 269 272