A new platform for Real-Time PCR detection of Salmonella spp., Listeria monocytogenes and Escherichia coli O157 in milk

Food Microbiology

Volumn 26, Issue 6, 2009, Pages 615-622

  Omiccioli, Enrica ^a,b   Amagliani, Giulia ^a   Brandi, Giorgio ^a   Magnani, Mauro ^a  

^a UNIVERSITY OF URBINO   (Italy)

^b Diatheva srl ^*  (Italy)

Author keywords

Food borne pathogens; HRM analysis; Multiple platform; Multiplex Real Time PCR; Simultaneous detection; Simultaneous growth medium

Indexed keywords

BACTERIAL DNA;

ANIMAL; ARTICLE; BACTERIAL COUNT; ESCHERICHIA COLI O157; FOOD CONTAMINATION; HUMAN; ISOLATION AND PURIFICATION; LISTERIA MONOCYTOGENES; METHODOLOGY; MICROBIOLOGY; MILK; POLYMERASE CHAIN REACTION; SALMONELLA; SENSITIVITY AND SPECIFICITY; SPECIES DIFFERENCE; TIME;

ANIMALS; COLONY COUNT, MICROBIAL; DNA, BACTERIAL; ESCHERICHIA COLI O157; FOOD CONTAMINATION; HUMANS; LISTERIA MONOCYTOGENES; MILK; POLYMERASE CHAIN REACTION; SALMONELLA; SENSITIVITY AND SPECIFICITY; SPECIES SPECIFICITY; TIME FACTORS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; LISTERIA MONOCYTOGENES; SALMONELLA;

EID: 67349286958     PISSN: 07400020     EISSN: 10959998     Source Type: Journal    
DOI: 10.1016/j.fm.2009.04.008     Document Type: Article

References (28)

1. Amagliani G., Omiccioli E., del Campo A., Bruce I.J., Brandi G., and Magnani M. Development of a magnetic capture hybridization-PCR assay for Listeria monocytogenes direct detection in milk samples. J. Appl. Microbiol. 100 2 (2006) 375-383
2. Anonymous. Microbiology of Food and Animal Feeding Stuffs - Polymerase Chain Reaction (PCR) for the Detection of Food-borne Pathogens - General Requirements and Definitions ISO 22174:2005 (2005), International Organization for Standardization, Geneva
3. Anonymous. Microbiology of Food and Animal Feeding Stuffs - Preparation of Test Samples, Initial Suspension and Decimal Dilutions for Microbiological Examination - Part 5: Specific Rules for the Preparation of Milk and Milk Products (2008), DIN EN ISO pp. 6887-6895
4. Aslam M., Hogan J., and Smith K.L. Development of a PCR-based assay to detect Shiga toxin-producing Escherichia coli, Listeria monocytogenes, and Salmonella in milk. Food Microbiol. 20 (2003) 345-350
5. Bohaychuk V.M., Gensler G.E., McFall M.E., King R.K., and Render D.G. A real-time PCR assay for the detection of Salmonella in a wide variety of food-animal matrices. J. Food Prot. 70 5 (2007) 1080-1087
6. Casabianca A., Orlandi C., Fraternale A., and Magnani M. A new one-step RT-PCR method for virus quantification in murine AIDS. J. Virol. Methods 110 (2003) 81-90
7. CDC. Outbreak of listeriosis associated with homemade Mexican-style cheese-North Carolina, October 2000-January 2001. Morb. Mortal. Wkly. Rep. 50 (2001) 560-562
8. CDC. Multistate outbreak of Salmonella serotype typhimurium infections associated with drinking unpasteurized milk-Illinois, Indiana, Ohio, and Tennessee, 2002-2003. Morb. Mortal. Wkly. Rep. 52 (2003) 613-615
9. EC. Council Directive 93/43/EEC of 14 June 1993 on the hygiene of foodstuffs. O. J. Eur. Comm. L 175 (1993) 1-11 19.07.1993
10. EEC, 2004. Regulation (EC) No. 853/2004 of the European parliament and of the Council of 29 April 2004 laying down specific hygiene rules for food of animal origin.
11. Elizaquível P., and Aznar R. A multiplex RTi-PCR reaction for simultaneous detection of Escherichia coli O157: h7, Salmonella spp. and Staphylococcus aureus on fresh, minimally processed vegetables. Food Microbiol. 25 5 (2008) 705-713
12. Elnifro E.M., Ashshi A.M., Cooper R.J., and Klapper P.E. Multiplex PCR: optimization and application in diagnostic virology. Clin. Microbiol. Rev. 13 (2000) 559-570
13. Fujikawa H., and Shimojima Y. Estimation of viable Salmonella cell numbers in meat and meat product using real-time PCR. J. Food Hyg. Soc. Jpn. 49 4 (2008) 261-265
14. Holicka J., Guy R.A., Kapoor A., Shepherd D., and Horgen P.A. A rapid (one day), sensitive real-time polymerase chain reaction assay for detecting Escherichia coli O157:H7 in ground beef. Can. J. Microbiol. 52 10 (2006) 992-998
15. Hyochin K., and Bhunia A.K. SEL, a multipathogen selective enrichment broth for simultaneous growth of Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes. Appl. Environ. Microbiol. 74 15 (2008) 4853-4866
16. Jinneman K.C., and Hill W.E. Listeria monocytogenes lineage group classification by MAMA-PCR of the listeriolysin gene. Curr. Microbiol. 43 (2001) 129-133
17. Kawasaki S., Horikoshi N., Okada Y., Takeshita K., Sameshima T., and Kawamoto S. Multiplex PCR for simultaneous detection of Salmonella spp., Listeria monocytogenes and Escherichia coli O157:H7 in meat samples. J. Food Prot. 68 (2005) 551-556
18. Koshkin A., Singh S.K., Nielsen P., Rajwanshi V.K., Kumar R., Meldgaard M., Olsen C.E., and Wengel J. LNA (Locked Nucleic Acids): synthesis of the adenine, cytosine, guanine, 5-methylcytosine, thymine and uracil bicyclonucleoside monomers, oligomerisation, and unprecedented nucleic acid recognition. Tetrahedron 54 (1998) 3607-3630
19. Latorra D., Arar K., and Hurley J.M. Design considerations and effects on LNA in PCR primers. Mol. Cell. Probes 17 (2003) 253-259
20. Malorny B., Hoorfar J., Bunge C., and Helmuth R. Multicentre validation of the analytical accuracy of Salmonella PCR: towards an international standard. Appl. Environ. Microbiol. 69 (2003) 290-296
21. Mazurek J., Salehi E., Propes D., Holt J., Bannerman T., Nicholson L.M., Bundesen M., Duffy R., and Moolenaar R.L. A multistate outbreak of Salmonella enterica serotype typhimurium infection linked to raw milk consumption-Ohio. J. Food Prot. 67 (2004) 2165-2170
22. Nordstrom J.L., Vickery M.C.L., Blackstone G.M., Murray S.L., and DePaola A. Development of a multiplex real-time PCR assay with an internal amplification control for the detection of total and pathogenic Vibrio parahaemolyticus bacteria in Oysters. Appl. Environ. Microbiol. 73 18 (2007) 5840-5847
23. Oliver S.P., Jayarao B.M., and Almeida R.A. Foodborne pathogens in milk and the dairy farm environment: food safety and public health implications. Foodborne Pathog. Dis. 2 (2005) 115-129
24. Omiccioli, E., Amagliani, Brandi, G., Bruce, I.J., Magnani, M. Simultaneous direct detection of Salmonella spp., Listeria monocytogenes, and Escherichia coli O157 in milk samples by magnetic extraction and multiplex PCR. J. Rapid Methods Automat. Microbiol., in press.
25. Proctor M.E., and Davis J.P. Escherichia coli O157:H7 infections in Wisconsin 1992-1999. Wis. Med. J. 99 (2000) 32-37
26. Reed B.A., and Grivetti L.E. Controlling on-farm inventories of bulk-tank raw milk-An opportunity to protect public health. J. Dairy Sci. 83 (2000) 2988-2991
27. Rossmanith P., Krassnig M., Wagner M., and Hein I. Detection of Listeria monocytogenes in food using a combined enrichment/real-time PCR method targeting the prfA gene. Res. Microbiol. 157 8 (2006) 763-771
28. Settanni L., and Corsetti A. The use of multiplex PCR to detect and differentiate food- and beverage-associated microorganisms: a review. J. Microbiol. Methods 69 (2007) 1-22