Influence of T-2 and HT-2 Toxin on the Blood-Brain Barrier In Vitro: New Experimental Hints for Neurotoxic Effects

PLoS ONE

Volumn 8, Issue 3, 2013, Pages

  Weidner, Maria ^a   Hüwel, Sabine ^a   Ebert, Franziska ^a   Schwerdtle, Tanja ^a   Galla, Hans Joachim ^a   Humpf, Hans Ulrich ^a  

^a UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

HT 2 TOXIN; OCCLUDIN; T 2 TOXIN;

ACTIVE TRANSPORT; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BARRIER INTEGRITY; BLOOD BRAIN BARRIER; CELL ASSAY; CELL VIABILITY; CONTROLLED STUDY; CYTOTOXICITY; ELECTRIC RESISTANCE; EXPERIMENTAL STUDY; IMMUNOCYTOCHEMISTRY; IN VITRO STUDY; MEMBRANE PERMEABILITY; NEUROTOXICITY; NONHUMAN; SWINE; TIGHT JUNCTION; TOXIN ANALYSIS; TOXIN STRUCTURE; TRANSENDOTHELIAL ELECTRICAL RESISTANCE;

ANIMALS; BLOOD-BRAIN BARRIER; CAPILLARIES; CELL DEATH; CELL MEMBRANE PERMEABILITY; CELL SURVIVAL; ELECTRIC IMPEDANCE; ENDOTHELIAL CELLS; IMMUNOHISTOCHEMISTRY; NEUROTOXINS; OCCLUDIN; SUCROSE; SUS SCROFA; T-2 TOXIN; TIME FACTORS;

EID: 84875447279     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0060484     Document Type: Article

References (36)

1. Foroud NA, Eudes F, (2009) Trichothecenes in Cereal Grains. Int J Mol Sci 10: 147-173.
2. Desjardins AE, Hohn TM, McCormick SP, (1993) Trichothecene biosynthesis in Fusarium species: chemistry, genetics, and significance. Microbiol Rev 57: 595-604.
3. Li Y, Wang Z, Beier RC, Shen J, Smet DD, De Saeger S, et al. (2011) T-2 Toxin, a trichothecene mycotoxin: Review of toxicity, metabolism, and analytical methods. J Agric Food Chem 59: 3441-3453.
4. Schothorst RC, van Egmond HP, (2004) Report from SCOOP task 3.2.10 "collection of occurrence data of Fusarium toxins in food and assessment of dietary intake by the population of EU member states". Toxicol Lett 153: 133-143.
5. Schollenberger M, Müller HM, Rüfle M, Suchy S, Plank S, et al. (2006) Natural Occurrence of 16 Fusarium Toxins in Grains and Feedstuffs of Plant Origin from Germany. Mycopathologia 161: 43-52.
6. Edwards SG, (2009) Fusarium mycotoxin content of UK organic and conventional oats. Food Addit Contam Part A 26: 1063-1069.
7. van der Fels-Klerx HJ, Stratakou I, (2010) T-2 toxin and HT-2 toxin in grain and grain-based commodities in Europe occurrence, factors affecting occurrence, co-occurrence and toxicological effects. World Mycotoxin J 3: 349-367.
8. Ohta M, Ishii K, Ueno Y, (1977) Metabolism of trichothecene mycotoxins. I. Microsomal deacetylation of T-2 toxin in animal tissues. J Biochem 82: 1591-1598.
9. EFSA (2011) Scientific Opinion on the risks for animal and public health related to the presence of T-2 and HT-2 toxin in food and feed. EFSA Journal 12.
10. Königs M, Mulac D, Schwerdt G, Gekle M, Humpf HU, (2009) Metabolism and cytotoxic effects of T-2 toxin and its metabolites on human cells in primary culture. Toxicology 258: 106-115.
11. Weidner M, Welsch T, Hübner F, Schwerdt G, Gekle M, et al. (2012) Identification and apoptotic potential of T-2 toxin metabolites in human cells. J Agric Food Chem 60: 5676-5684.
12. Sudakin D, (2003) Trichothecenes in the environment: relevance to human health. Toxicol Lett 143: 97-107.
13. Bouaziz C, Sharaf El Dein O, El Golli E, Abid-Essefi S, Brenner C, et al. (2008) Different apoptotic pathways induced by zearalenone, T-2 toxin and ochratoxin A in human hepatoma cells. Toxicology 254: 19-28.
14. Chaudhari M, Jayaraj R, Bhaskar ASB, Lakshmana Rao PV, (2009) Oxidative stress induction by T-2 toxin causes DNA damage and triggers apoptosis via caspase pathway in human cervical cancer cells. Toxicology 262: 153-161.
15. Fairhurst S, Marrs TC, Parker HC, Scawin JW, Swanston DW, (1987) Acute toxicity of T-2 toxin in rats, mice, guinea-pigs, and pigeons. Toxicology 43: 31-49.
16. Li M, Cuff CF, Pestka JJ, (2006) T-2 toxin impairment of enteric reovirus clearance in the mouse associated with suppressed immunoglobulin and IFN-gamma responses. Toxicol Appl Pharmacol 214: 318-325.
17. Chaudhary M, Rao PV, (2010) Brain oxidative stress after dermal and subcutaneous exposure of T-2 toxin in mice. Food Chem Toxicol 48: 3436-3442.
18. Wang J, Fitzpatrick DW, Wilson JR, (1998) Effects of the trichothecene mycotoxin T-2 toxin on neurotransmitters and metabolites in discrete areas of the rat brain. Food Chem Toxicol 36: 947-953.
19. Sirkka U, Nieminen SA, Ylitalo P, (1992) Acute neurobehavioral toxicity of trichothecene T-2 toxin in the rat. Pharmacology & Toxicology 70: 111-114.
20. Ravindran J, Agrawal M, Gupta N, Lakshmana Rao PV, (2011) Alteration of blood brain barrier permeability by T-2 toxin: Role of MMP-9 and inflammatory cytokines. Toxicology 280: 44-52.
21. Doi K, Uetsuka K, (2011) Mechanisms of mycotoxin-induced neurotoxicity through oxidative stress-associated pathways. Int J Mol Sci 12: 5213-5237.
22. Abbott NJ, Patabendige AA, Dolman DE, Yusof SR, Begley DJ, (2010) Structure and function of the blood-brain barrier. Neurobiol Dis 37: 13-25.
23. Abbott NJ, Ronnback L, Hansson E, (2006) Astrocyte-endothelial interactions at the blood-brain barrier. Nat Rev Neurosci 7: 41-53.
24. Weidner M, Lenczyk M, Schwerdt G, Gekle M, Humpf HU (2013) Neurotoxic potential and cellular uptake of T-2 toxin in human astrocytes in primary culture. Chem Res Toxicol doi:10.1021/tx3004664.
25. Wu Q, Engemann A, Cramer B, Welsch T, Yuan Z, et al. (2012) Intestinal metabolism of T-2 toxin in the pig cecum model. Mycotoxin Research 28: 191-198.
26. Franke H, Galla HJ, Beuckmann CT, (2000) Primary cultures of brain microvessel endothelial cells: a valid and flexible model to study drug transport through the blood-brain barrier in vitro. Brain Res Brain Res Protoc 5: 248-256.
27. Crone C, Olesen SP, (1982) Electrical resistance of brain microvascular endothelium. Brain Res 241: 49-55.
28. Hoheisel D, Nitz T, Franke H, Wegener J, Hakvoort A, et al. (1998) Hydrocortisone reinforces the blood-brain properties in a serum free cell culture system. Biochem Biophys Res Commun 247: 312-315.
29. Mulac D, Huwel S, Galla HJ, Humpf HU, (2012) Permeability of ergot alkaloids across the blood-brain barrier in vitro and influence on the barrier integrity. Mol Nutr Food Res 56: 475-485.
30. Beyer M, Ferse I, Humpf HU, (2009) Large-scale production of selected type A trichothecenes: the use of HT-2 toxin and T-2 triol as precursors for the synthesis of d3-T-2 and d3-HT-2 toxin. Mycotoxin Res 25: 41-52.
31. Kroll S, El-Gindi J, Thanabalasundaram G, Panpumthong P, Schrot S, et al. (2009) Control of the blood-brain barrier by glucocorticoids and the cells of the neurovascular unit. Ann NY Acad Sci 1165: 228-239.
32. Rempe R, Cramer S, Huwel S, Galla HJ, (2011) Transport of Poly(n-butylcyano-acrylate) nanoparticles across the blood-brain barrier in vitro and their influence on barrier integrity. Biochem Biophys Res Commun 406: 64-69.
33. Lischper M, Beuck S, Thanabalasundaram G, Pieper C, Galla H-J, (2010) Metalloproteinase mediated occludin cleavage in the cerebral microcapillary endothelium under pathological conditions. Brain Research 1326: 114-127.
34. Franke H, Galla HJ, Beuckmann CT, (1999) An improved low-permeability in vitro-model of the blood-brain barrier: transport studies on retinoids, sucrose, haloperidol, caffeine and mannitol. Brain Res 818: 65-71.
35. DeLean A, Munson PJ, Rodbard D, (1978) Simultaneous analysis of families of sigmoidal curves: application to bioassay, radioligand assay, and physiological dose-response curves. Am J Physiol 235: E97-102.
36. Lohmann C, Huwel S, Galla HJ, (2002) Predicting blood-brain barrier permeability of drugs: evaluation of different in vitro assays. J Drug Target 10: 263-276.