메뉴 건너뛰기




Volumn 14, Issue 2, 2013, Pages 3456-3466

Validation of reference genes for the determination of platelet transcript level in healthy individuals and in patients with the history of myocardial infarction

Author keywords

Normalization; Platelet; Reference gene; RT qPCR; Transcript level

Indexed keywords

BETA ACTIN; COFILIN 1; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; GUANINE NUCLEOTIDE BINDING PROTEIN ALPHA SUBUNIT; HEPATOMA DERIVED GROWTH FACTOR;

EID: 84875089327     PISSN: 16616596     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/ijms14023456     Document Type: Article
Times cited : (22)

References (33)
  • 1
    • 0033775615 scopus 로고    scopus 로고
    • Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays
    • Bustin, S.A. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J. Mol. Endocrinol. 2000, 25, 169-193.
    • (2000) J. Mol. Endocrinol. , vol.25 , pp. 169-193
    • Bustin, S.A.1
  • 2
    • 3142615272 scopus 로고    scopus 로고
    • Validation of housekeeping genes for normalizing RNA expression in real-time PCR
    • Dheda, K.; Huggett, J.F.; Bustin, S.A.; Johnson, M.A.; Rook, G.; Zumla, A. Validation of housekeeping genes for normalizing RNA expression in real-time PCR. Biotechniques 2004, 37, 112-114, 116, 118-119.
    • (2004) Biotechniques , vol.37
    • Dheda, K.1    Huggett, J.F.2    Bustin, S.A.3    Johnson, M.A.4    Rook, G.5    Zumla, A.6
  • 4
    • 0037129827 scopus 로고    scopus 로고
    • Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
    • RESEARCH0034
    • Vandesompele, J.; de Preter, K.; Pattyn, F.; Poppe, B.; van Roy, N.; de Paepe, A.; Speleman, F. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 2002, 3, RESEARCH0034.
    • (2002) Genome Biol. , vol.3
    • Vandesompele, J.1    de Preter, K.2    Pattyn, F.3    Poppe, B.4    van Roy, N.5    de Paepe, A.6    Speleman, F.7
  • 5
    • 33847643776 scopus 로고    scopus 로고
    • Quantification of mRNA using real-time RT-PCR
    • Nolan, T.; Hands, R.E.; Bustin, S.A. Quantification of mRNA using real-time RT-PCR. Nat. Protoc. 2006, 1, 1559-1582.
    • (2006) Nat. Protoc. , vol.1 , pp. 1559-1582
    • Nolan, T.1    Hands, R.E.2    Bustin, S.A.3
  • 6
    • 10244249152 scopus 로고    scopus 로고
    • Platelets synthesize large amounts of active plasminogen activator inhibitor 1
    • Brogren, H.; Karlsson, L.; Andersson, M.; Wang, L.; Erlinge, D.; Jern, S. Platelets synthesize large amounts of active plasminogen activator inhibitor 1. Blood 2004, 104, 3943-3948.
    • (2004) Blood , vol.104 , pp. 3943-3948
    • Brogren, H.1    Karlsson, L.2    Andersson, M.3    Wang, L.4    Erlinge, D.5    Jern, S.6
  • 8
    • 9244257903 scopus 로고    scopus 로고
    • Gene expression analysis in platelets from a single donor: Evaluation of a PCR-based amplification technique
    • Rox, J.M.; Bugert, P.; Muller, J.; Schorr, A.; Hanfland, P.; Madlener, K.; Kluter, H.; Potzsch, B. Gene expression analysis in platelets from a single donor: Evaluation of a PCR-based amplification technique. Clin. Chem. 2004, 50, 2271-2278.
    • (2004) Clin. Chem. , vol.50 , pp. 2271-2278
    • Rox, J.M.1    Bugert, P.2    Muller, J.3    Schorr, A.4    Hanfland, P.5    Madlener, K.6    Kluter, H.7    Potzsch, B.8
  • 9
    • 42649146229 scopus 로고    scopus 로고
    • Gene expression profiling for the identification of G-protein coupled receptors in human platelets
    • Amisten, S.; Braun, O.O.; Bengtsson, A.; Erlinge, D. Gene expression profiling for the identification of G-protein coupled receptors in human platelets. Thromb. Res. 2008, 122, 47-57.
    • (2008) Thromb. Res. , vol.122 , pp. 47-57
    • Amisten, S.1    Braun, O.O.2    Bengtsson, A.3    Erlinge, D.4
  • 10
    • 84555191630 scopus 로고    scopus 로고
    • A rapid and efficient platelet purification protocol for platelet gene expression studies
    • Amisten, S. A rapid and efficient platelet purification protocol for platelet gene expression studies. Methods Mol. Biol. 2012, 788, 155-172.
    • (2012) Methods Mol. Biol. , vol.788 , pp. 155-172
    • Amisten, S.1
  • 11
    • 34548573167 scopus 로고    scopus 로고
    • JAK2-V617F mutation analysis of granulocytes and platelets from patients with chronic myeloproliferative disorders: Advantage of studying platelets
    • Toyama, K.; Karasawa, M.; Yamane, A.; Irisawa, H.; Yokohama, A.; Saitoh, T.; Handa, H.; Matsushima, T.; Sawamura, M.; Miyawaki, S.; et al. JAK2-V617F mutation analysis of granulocytes and platelets from patients with chronic myeloproliferative disorders: Advantage of studying platelets. Br. J. Haematol. 2007, 139, 64-69.
    • (2007) Br. J. Haematol. , vol.139 , pp. 64-69
    • Toyama, K.1    Karasawa, M.2    Yamane, A.3    Irisawa, H.4    Yokohama, A.5    Saitoh, T.6    Handa, H.7    Matsushima, T.8    Sawamura, M.9    Miyawaki, S.10
  • 12
    • 3442876969 scopus 로고    scopus 로고
    • Normalization of real-time quantitative reverse transcription-PCR data: A model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets
    • Andersen, C.L.; Jensen, J.L.; Orntoft, T.F. Normalization of real-time quantitative reverse transcription-PCR data: A model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res. 2004, 64, 5245-5250.
    • (2004) Cancer Res. , vol.64 , pp. 5245-5250
    • Andersen, C.L.1    Jensen, J.L.2    Orntoft, T.F.3
  • 13
    • 33750295976 scopus 로고    scopus 로고
    • Selection of housekeeping genes for gene expression studies in human reticulocytes using real-time PCR
    • Silver, N.; Best, S.; Jiang, J.; Thein, S.L. Selection of housekeeping genes for gene expression studies in human reticulocytes using real-time PCR. BMC Mol. Biol. 2006, 7, 33.
    • (2006) BMC Mol. Biol. , vol.7 , pp. 33
    • Silver, N.1    Best, S.2    Jiang, J.3    Thein, S.L.4
  • 14
    • 1842483281 scopus 로고    scopus 로고
    • Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper-Excel-based tool using pair-wise correlations
    • Pfaffl, M.W.; Tichopad, A.; Prgomet, C.; Neuvians, T.P. Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper-Excel-based tool using pair-wise correlations. Biotechnol. Lett. 2004, 26, 509-515.
    • (2004) Biotechnol. Lett. , vol.26 , pp. 509-515
    • Pfaffl, M.W.1    Tichopad, A.2    Prgomet, C.3    Neuvians, T.P.4
  • 15
    • 84865653135 scopus 로고    scopus 로고
    • Evaluation of new reference genes in papaya for accurate transcript normalization under different experimental conditions
    • Zhu, X.; Li, X.; Chen, W.; Chen, J.; Lu, W.; Chen, L.; Fu, D. Evaluation of new reference genes in papaya for accurate transcript normalization under different experimental conditions. PLoS One 2012, 7, e44405.
    • (2012) PLoS One , vol.7
    • Zhu, X.1    Li, X.2    Chen, W.3    Chen, J.4    Lu, W.5    Chen, L.6    Fu, D.7
  • 17
    • 77049118791 scopus 로고    scopus 로고
    • Validation of internal reference genes for quantitative real-time PCR in a non-model organism, the yellow-necked mouse, Apodemus flavicollis
    • Axtner, J.; Sommer, S. Validation of internal reference genes for quantitative real-time PCR in a non-model organism, the yellow-necked mouse, Apodemus flavicollis. BMC Res. Notes 2009, 2, 264.
    • (2009) BMC Res. Notes , vol.2 , pp. 264
    • Axtner, J.1    Sommer, S.2
  • 18
    • 79957445919 scopus 로고    scopus 로고
    • Selection of reference genes for normalization of quantitative real-time PCR in organ culture of the rat and rabbit intervertebral disc
    • Seol, D.; Choe, H.; Zheng, H.; Jang, K.; Ramakrishnan, P.S.; Lim, T.H.; Martin, J.A. Selection of reference genes for normalization of quantitative real-time PCR in organ culture of the rat and rabbit intervertebral disc. BMC Res. Notes 2011, 4, 162.
    • (2011) BMC Res. Notes , vol.4 , pp. 162
    • Seol, D.1    Choe, H.2    Zheng, H.3    Jang, K.4    Ramakrishnan, P.S.5    Lim, T.H.6    Martin, J.A.7
  • 19
    • 84860322720 scopus 로고    scopus 로고
    • Selection of reference genes for quantitative real-time RT-PCR analysis in citrus
    • Yan, J.; Yuan, F.; Long, G.; Qin, L.; Deng, Z. Selection of reference genes for quantitative real-time RT-PCR analysis in citrus. Mol. Biol. Rep. 2012, 39, 1831-1838.
    • (2012) Mol. Biol. Rep. , vol.39 , pp. 1831-1838
    • Yan, J.1    Yuan, F.2    Long, G.3    Qin, L.4    Deng, Z.5
  • 22
    • 33645293441 scopus 로고    scopus 로고
    • SPUD: A quantitative PCR assay for the detection of inhibitors in nucleic acid preparations
    • Nolan, T.; Hands, R.E.; Ogunkolade, W.; Bustin, S.A. SPUD: A quantitative PCR assay for the detection of inhibitors in nucleic acid preparations. Anal. Biochem. 2006, 351, 308-310.
    • (2006) Anal. Biochem. , vol.351 , pp. 308-310
    • Nolan, T.1    Hands, R.E.2    Ogunkolade, W.3    Bustin, S.A.4
  • 23
    • 42649094850 scopus 로고    scopus 로고
    • Use of functional highly purified human platelets for the identification of new proteins of the IPP signaling pathway
    • Birschmann, I.; Mietner, S.; Dittrich, M.; Pfrang, J.; Dandekar, T.; Walter, U. Use of functional highly purified human platelets for the identification of new proteins of the IPP signaling pathway. Thromb. Res. 2008, 122, 59-68.
    • (2008) Thromb. Res. , vol.122 , pp. 59-68
    • Birschmann, I.1    Mietner, S.2    Dittrich, M.3    Pfrang, J.4    Dandekar, T.5    Walter, U.6
  • 24
    • 44349158988 scopus 로고    scopus 로고
    • How many human genes can be defined as housekeeping with current expression data?
    • Zhu, J.; He, F.; Song, S.; Wang, J.; Yu, J. How many human genes can be defined as housekeeping with current expression data? BMC Genomics 2008, 9, 172.
    • (2008) BMC Genomics , vol.9 , pp. 172
    • Zhu, J.1    He, F.2    Song, S.3    Wang, J.4    Yu, J.5
  • 26
    • 17344392308 scopus 로고    scopus 로고
    • A new mathematical model for relative quantification in real-time RT-PCR
    • Pfaffl, M.W. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 2001, 29, e45.
    • (2001) Nucleic Acids Res. , vol.29
    • Pfaffl, M.W.1
  • 28
    • 4644368801 scopus 로고    scopus 로고
    • Peptidylpropyl isomerase B (PPIB): A suitable reference gene for mRNA quantification in peripheral whole blood
    • Pachot, A.; Blond, J.L.; Mougin, B.; Miossec, P. Peptidylpropyl isomerase B (PPIB): A suitable reference gene for mRNA quantification in peripheral whole blood. J. Biotechnol. 2004, 114, 121-124.
    • (2004) J. Biotechnol. , vol.114 , pp. 121-124
    • Pachot, A.1    Blond, J.L.2    Mougin, B.3    Miossec, P.4
  • 29
    • 76449109526 scopus 로고    scopus 로고
    • Validation of a primer optimisation matrix to improve the performance of reverse transcription-Quantitative real-time PCR assays
    • Mikeska, T.; Dobrovic, A. Validation of a primer optimisation matrix to improve the performance of reverse transcription-Quantitative real-time PCR assays. BMC Res. Notes 2009, 2, 112.
    • (2009) BMC Res. Notes , vol.2 , pp. 112
    • Mikeska, T.1    Dobrovic, A.2
  • 31
    • 78651458417 scopus 로고    scopus 로고
    • Available online: (accessed on 5 February 2013)
    • Universal ProbeLibrary System. Available online: https://www.roche-applied-science.com/sis/ rtpcr/upl/index.jsp (accessed on 5 February 2013).
    • Universal ProbeLibrary System.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.