-
1
-
-
0033775615
-
Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays
-
Bustin, S.A. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J. Mol. Endocrinol. 2000, 25, 169-193.
-
(2000)
J. Mol. Endocrinol.
, vol.25
, pp. 169-193
-
-
Bustin, S.A.1
-
2
-
-
3142615272
-
Validation of housekeeping genes for normalizing RNA expression in real-time PCR
-
Dheda, K.; Huggett, J.F.; Bustin, S.A.; Johnson, M.A.; Rook, G.; Zumla, A. Validation of housekeeping genes for normalizing RNA expression in real-time PCR. Biotechniques 2004, 37, 112-114, 116, 118-119.
-
(2004)
Biotechniques
, vol.37
-
-
Dheda, K.1
Huggett, J.F.2
Bustin, S.A.3
Johnson, M.A.4
Rook, G.5
Zumla, A.6
-
3
-
-
41149171125
-
Evidence based selection of housekeeping genes
-
De Jonge, H.J.; Fehrmann, R.S.; de Bont, E.S.; Hofstra, R.M.; Gerbens, F.; Kamps, W.A.; de Vries, E.G.; van der Zee, A.G.; te Meerman, G.J.; ter Elst, A. Evidence based selection of housekeeping genes. PLoS One 2007, 2, e898.
-
(2007)
PLoS One
, vol.2
-
-
De Jonge, H.J.1
Fehrmann, R.S.2
de Bont, E.S.3
Hofstra, R.M.4
Gerbens, F.5
Kamps, W.A.6
de Vries, E.G.7
van der Zee, A.G.8
te Meerman, G.J.9
ter Elst, A.10
-
4
-
-
0037129827
-
Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
-
RESEARCH0034
-
Vandesompele, J.; de Preter, K.; Pattyn, F.; Poppe, B.; van Roy, N.; de Paepe, A.; Speleman, F. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 2002, 3, RESEARCH0034.
-
(2002)
Genome Biol.
, vol.3
-
-
Vandesompele, J.1
de Preter, K.2
Pattyn, F.3
Poppe, B.4
van Roy, N.5
de Paepe, A.6
Speleman, F.7
-
5
-
-
33847643776
-
Quantification of mRNA using real-time RT-PCR
-
Nolan, T.; Hands, R.E.; Bustin, S.A. Quantification of mRNA using real-time RT-PCR. Nat. Protoc. 2006, 1, 1559-1582.
-
(2006)
Nat. Protoc.
, vol.1
, pp. 1559-1582
-
-
Nolan, T.1
Hands, R.E.2
Bustin, S.A.3
-
6
-
-
10244249152
-
Platelets synthesize large amounts of active plasminogen activator inhibitor 1
-
Brogren, H.; Karlsson, L.; Andersson, M.; Wang, L.; Erlinge, D.; Jern, S. Platelets synthesize large amounts of active plasminogen activator inhibitor 1. Blood 2004, 104, 3943-3948.
-
(2004)
Blood
, vol.104
, pp. 3943-3948
-
-
Brogren, H.1
Karlsson, L.2
Andersson, M.3
Wang, L.4
Erlinge, D.5
Jern, S.6
-
7
-
-
36949037990
-
Alternative splicing of platelet cyclooxygenase-2 mRNA in patients after coronary artery bypass grafting
-
Censarek, P.; Steger, G.; Paolini, C.; Hohlfeld, T.; Grosser, T.; Zimmermann, N.; Fleckenstein, D.; Schror, K.; Weber, A.A. Alternative splicing of platelet cyclooxygenase-2 mRNA in patients after coronary artery bypass grafting. Thromb. Haemost. 2007, 98, 1309-1315.
-
(2007)
Thromb. Haemost.
, vol.98
, pp. 1309-1315
-
-
Censarek, P.1
Steger, G.2
Paolini, C.3
Hohlfeld, T.4
Grosser, T.5
Zimmermann, N.6
Fleckenstein, D.7
Schror, K.8
Weber, A.A.9
-
8
-
-
9244257903
-
Gene expression analysis in platelets from a single donor: Evaluation of a PCR-based amplification technique
-
Rox, J.M.; Bugert, P.; Muller, J.; Schorr, A.; Hanfland, P.; Madlener, K.; Kluter, H.; Potzsch, B. Gene expression analysis in platelets from a single donor: Evaluation of a PCR-based amplification technique. Clin. Chem. 2004, 50, 2271-2278.
-
(2004)
Clin. Chem.
, vol.50
, pp. 2271-2278
-
-
Rox, J.M.1
Bugert, P.2
Muller, J.3
Schorr, A.4
Hanfland, P.5
Madlener, K.6
Kluter, H.7
Potzsch, B.8
-
9
-
-
42649146229
-
Gene expression profiling for the identification of G-protein coupled receptors in human platelets
-
Amisten, S.; Braun, O.O.; Bengtsson, A.; Erlinge, D. Gene expression profiling for the identification of G-protein coupled receptors in human platelets. Thromb. Res. 2008, 122, 47-57.
-
(2008)
Thromb. Res.
, vol.122
, pp. 47-57
-
-
Amisten, S.1
Braun, O.O.2
Bengtsson, A.3
Erlinge, D.4
-
10
-
-
84555191630
-
A rapid and efficient platelet purification protocol for platelet gene expression studies
-
Amisten, S. A rapid and efficient platelet purification protocol for platelet gene expression studies. Methods Mol. Biol. 2012, 788, 155-172.
-
(2012)
Methods Mol. Biol.
, vol.788
, pp. 155-172
-
-
Amisten, S.1
-
11
-
-
34548573167
-
JAK2-V617F mutation analysis of granulocytes and platelets from patients with chronic myeloproliferative disorders: Advantage of studying platelets
-
Toyama, K.; Karasawa, M.; Yamane, A.; Irisawa, H.; Yokohama, A.; Saitoh, T.; Handa, H.; Matsushima, T.; Sawamura, M.; Miyawaki, S.; et al. JAK2-V617F mutation analysis of granulocytes and platelets from patients with chronic myeloproliferative disorders: Advantage of studying platelets. Br. J. Haematol. 2007, 139, 64-69.
-
(2007)
Br. J. Haematol.
, vol.139
, pp. 64-69
-
-
Toyama, K.1
Karasawa, M.2
Yamane, A.3
Irisawa, H.4
Yokohama, A.5
Saitoh, T.6
Handa, H.7
Matsushima, T.8
Sawamura, M.9
Miyawaki, S.10
-
12
-
-
3442876969
-
Normalization of real-time quantitative reverse transcription-PCR data: A model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets
-
Andersen, C.L.; Jensen, J.L.; Orntoft, T.F. Normalization of real-time quantitative reverse transcription-PCR data: A model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res. 2004, 64, 5245-5250.
-
(2004)
Cancer Res.
, vol.64
, pp. 5245-5250
-
-
Andersen, C.L.1
Jensen, J.L.2
Orntoft, T.F.3
-
13
-
-
33750295976
-
Selection of housekeeping genes for gene expression studies in human reticulocytes using real-time PCR
-
Silver, N.; Best, S.; Jiang, J.; Thein, S.L. Selection of housekeeping genes for gene expression studies in human reticulocytes using real-time PCR. BMC Mol. Biol. 2006, 7, 33.
-
(2006)
BMC Mol. Biol.
, vol.7
, pp. 33
-
-
Silver, N.1
Best, S.2
Jiang, J.3
Thein, S.L.4
-
14
-
-
1842483281
-
Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper-Excel-based tool using pair-wise correlations
-
Pfaffl, M.W.; Tichopad, A.; Prgomet, C.; Neuvians, T.P. Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper-Excel-based tool using pair-wise correlations. Biotechnol. Lett. 2004, 26, 509-515.
-
(2004)
Biotechnol. Lett.
, vol.26
, pp. 509-515
-
-
Pfaffl, M.W.1
Tichopad, A.2
Prgomet, C.3
Neuvians, T.P.4
-
15
-
-
84865653135
-
Evaluation of new reference genes in papaya for accurate transcript normalization under different experimental conditions
-
Zhu, X.; Li, X.; Chen, W.; Chen, J.; Lu, W.; Chen, L.; Fu, D. Evaluation of new reference genes in papaya for accurate transcript normalization under different experimental conditions. PLoS One 2012, 7, e44405.
-
(2012)
PLoS One
, vol.7
-
-
Zhu, X.1
Li, X.2
Chen, W.3
Chen, J.4
Lu, W.5
Chen, L.6
Fu, D.7
-
16
-
-
64149097786
-
The MIQE guidelines: Minimum information for publication of quantitative real-time PCR experiments
-
Bustin, S.A.; Benes, V.; Garson, J.A.; Hellemans, J.; Huggett, J.; Kubista, M.; Mueller, R.; Nolan, T.; Pfaffl, M.W.; Shipley, G.L.; et al. The MIQE guidelines: Minimum information for publication of quantitative real-time PCR experiments. Clin. Chem. 2009, 55, 611-622.
-
(2009)
Clin. Chem.
, vol.55
, pp. 611-622
-
-
Bustin, S.A.1
Benes, V.2
Garson, J.A.3
Hellemans, J.4
Huggett, J.5
Kubista, M.6
Mueller, R.7
Nolan, T.8
Pfaffl, M.W.9
Shipley, G.L.10
-
17
-
-
77049118791
-
Validation of internal reference genes for quantitative real-time PCR in a non-model organism, the yellow-necked mouse, Apodemus flavicollis
-
Axtner, J.; Sommer, S. Validation of internal reference genes for quantitative real-time PCR in a non-model organism, the yellow-necked mouse, Apodemus flavicollis. BMC Res. Notes 2009, 2, 264.
-
(2009)
BMC Res. Notes
, vol.2
, pp. 264
-
-
Axtner, J.1
Sommer, S.2
-
18
-
-
79957445919
-
Selection of reference genes for normalization of quantitative real-time PCR in organ culture of the rat and rabbit intervertebral disc
-
Seol, D.; Choe, H.; Zheng, H.; Jang, K.; Ramakrishnan, P.S.; Lim, T.H.; Martin, J.A. Selection of reference genes for normalization of quantitative real-time PCR in organ culture of the rat and rabbit intervertebral disc. BMC Res. Notes 2011, 4, 162.
-
(2011)
BMC Res. Notes
, vol.4
, pp. 162
-
-
Seol, D.1
Choe, H.2
Zheng, H.3
Jang, K.4
Ramakrishnan, P.S.5
Lim, T.H.6
Martin, J.A.7
-
19
-
-
84860322720
-
Selection of reference genes for quantitative real-time RT-PCR analysis in citrus
-
Yan, J.; Yuan, F.; Long, G.; Qin, L.; Deng, Z. Selection of reference genes for quantitative real-time RT-PCR analysis in citrus. Mol. Biol. Rep. 2012, 39, 1831-1838.
-
(2012)
Mol. Biol. Rep.
, vol.39
, pp. 1831-1838
-
-
Yan, J.1
Yuan, F.2
Long, G.3
Qin, L.4
Deng, Z.5
-
20
-
-
77249119389
-
Validation of suitable internal control genes for expression studies in aging
-
Zampieri, M.; Ciccarone, F.; Guastafierro, T.; Bacalini, M.G.; Calabrese, R.; Moreno-Villanueva, M.; Reale, A.; Chevanne, M.; Burkle, A.; Caiafa, P. Validation of suitable internal control genes for expression studies in aging. Mech. Ageing Dev. 2010, 131, 89-95.
-
(2010)
Mech. Ageing Dev.
, vol.131
, pp. 89-95
-
-
Zampieri, M.1
Ciccarone, F.2
Guastafierro, T.3
Bacalini, M.G.4
Calabrese, R.5
Moreno-Villanueva, M.6
Reale, A.7
Chevanne, M.8
Burkle, A.9
Caiafa, P.10
-
21
-
-
68149154784
-
A longitudinal study of gene expression in healthy individuals
-
Karlovich, C.; Duchateau-Nguyen, G.; Johnson, A.; McLoughlin, P.; Navarro, M.; Fleurbaey, C.; Steiner, L.; Tessier, M.; Nguyen, T.; Wilhelm-Seiler, M.; et al. A longitudinal study of gene expression in healthy individuals. BMC Med. Genomics 2009, 2, 33.
-
(2009)
BMC Med. Genomics
, vol.2
, pp. 33
-
-
Karlovich, C.1
Duchateau-Nguyen, G.2
Johnson, A.3
McLoughlin, P.4
Navarro, M.5
Fleurbaey, C.6
Steiner, L.7
Tessier, M.8
Nguyen, T.9
Wilhelm-Seiler, M.10
-
22
-
-
33645293441
-
SPUD: A quantitative PCR assay for the detection of inhibitors in nucleic acid preparations
-
Nolan, T.; Hands, R.E.; Ogunkolade, W.; Bustin, S.A. SPUD: A quantitative PCR assay for the detection of inhibitors in nucleic acid preparations. Anal. Biochem. 2006, 351, 308-310.
-
(2006)
Anal. Biochem.
, vol.351
, pp. 308-310
-
-
Nolan, T.1
Hands, R.E.2
Ogunkolade, W.3
Bustin, S.A.4
-
23
-
-
42649094850
-
Use of functional highly purified human platelets for the identification of new proteins of the IPP signaling pathway
-
Birschmann, I.; Mietner, S.; Dittrich, M.; Pfrang, J.; Dandekar, T.; Walter, U. Use of functional highly purified human platelets for the identification of new proteins of the IPP signaling pathway. Thromb. Res. 2008, 122, 59-68.
-
(2008)
Thromb. Res.
, vol.122
, pp. 59-68
-
-
Birschmann, I.1
Mietner, S.2
Dittrich, M.3
Pfrang, J.4
Dandekar, T.5
Walter, U.6
-
24
-
-
44349158988
-
How many human genes can be defined as housekeeping with current expression data?
-
Zhu, J.; He, F.; Song, S.; Wang, J.; Yu, J. How many human genes can be defined as housekeeping with current expression data? BMC Genomics 2008, 9, 172.
-
(2008)
BMC Genomics
, vol.9
, pp. 172
-
-
Zhu, J.1
He, F.2
Song, S.3
Wang, J.4
Yu, J.5
-
25
-
-
77649302072
-
Expressed Alu repeats as a novel, reliable tool for normalization of real-time quantitative RT-PCR data
-
Marullo, M.; Zuccato, C.; Mariotti, C.; Lahiri, N.; Tabrizi, S.J.; di Donato, S.; Cattaneo, E. Expressed Alu repeats as a novel, reliable tool for normalization of real-time quantitative RT-PCR data. Genome Biol. 2010, 11, R9.
-
(2010)
Genome Biol.
, vol.11
-
-
Marullo, M.1
Zuccato, C.2
Mariotti, C.3
Lahiri, N.4
Tabrizi, S.J.5
di Donato, S.6
Cattaneo, E.7
-
26
-
-
17344392308
-
A new mathematical model for relative quantification in real-time RT-PCR
-
Pfaffl, M.W. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 2001, 29, e45.
-
(2001)
Nucleic Acids Res.
, vol.29
-
-
Pfaffl, M.W.1
-
27
-
-
84900918601
-
-
Springer: Dordrecht, The Netherlands
-
Pestana, E.A.; Belak, S.; Diallo, A.; Crowther, J.R.; Viljoen, G.J. Early, Rapid and Sensitive Veterinary Molecular Diagnostics-Real Time PCR Applications; Springer: Dordrecht, The Netherlands, 2010.
-
(2010)
Early, Rapid and Sensitive Veterinary Molecular Diagnostics-Real Time PCR Applications
-
-
Pestana, E.A.1
Belak, S.2
Diallo, A.3
Crowther, J.R.4
Viljoen, G.J.5
-
28
-
-
4644368801
-
Peptidylpropyl isomerase B (PPIB): A suitable reference gene for mRNA quantification in peripheral whole blood
-
Pachot, A.; Blond, J.L.; Mougin, B.; Miossec, P. Peptidylpropyl isomerase B (PPIB): A suitable reference gene for mRNA quantification in peripheral whole blood. J. Biotechnol. 2004, 114, 121-124.
-
(2004)
J. Biotechnol.
, vol.114
, pp. 121-124
-
-
Pachot, A.1
Blond, J.L.2
Mougin, B.3
Miossec, P.4
-
29
-
-
76449109526
-
Validation of a primer optimisation matrix to improve the performance of reverse transcription-Quantitative real-time PCR assays
-
Mikeska, T.; Dobrovic, A. Validation of a primer optimisation matrix to improve the performance of reverse transcription-Quantitative real-time PCR assays. BMC Res. Notes 2009, 2, 112.
-
(2009)
BMC Res. Notes
, vol.2
, pp. 112
-
-
Mikeska, T.1
Dobrovic, A.2
-
30
-
-
70349653541
-
Design and optimization of reverse-transcription quantitative PCR experiments
-
Tichopad, A.; Kitchen, R.; Riedmaier, I.; Becker, C.; Stahlberg, A.; Kubista, M. Design and optimization of reverse-transcription quantitative PCR experiments. Clin. Chem. 2009, 55, 1816-1823.
-
(2009)
Clin. Chem.
, vol.55
, pp. 1816-1823
-
-
Tichopad, A.1
Kitchen, R.2
Riedmaier, I.3
Becker, C.4
Stahlberg, A.5
Kubista, M.6
-
31
-
-
78651458417
-
-
Available online: (accessed on 5 February 2013)
-
Universal ProbeLibrary System. Available online: https://www.roche-applied-science.com/sis/ rtpcr/upl/index.jsp (accessed on 5 February 2013).
-
Universal ProbeLibrary System.
-
-
-
32
-
-
58149196189
-
RTPrimerDB: The portal for real-time PCR primers and probes
-
Lefever, S.; Vandesompele, J.; Speleman, F.; Pattyn, F. RTPrimerDB: The portal for real-time PCR primers and probes. Nucleic Acids Res. 2009, 37, D942-D945.
-
(2009)
Nucleic Acids Res.
, vol.37
-
-
Lefever, S.1
Vandesompele, J.2
Speleman, F.3
Pattyn, F.4
-
33
-
-
34547591343
-
Primer3Plus, an enhanced web interface to Primer3
-
Untergasser, A.; Nijveen, H.; Rao, X.; Bisseling, T.; Geurts, R.; Leunissen, J.A. Primer3Plus, an enhanced web interface to Primer3. Nucleic Acids Res. 2007, 35, W71-W74.
-
(2007)
Nucleic Acids Res.
, vol.35
-
-
Untergasser, A.1
Nijveen, H.2
Rao, X.3
Bisseling, T.4
Geurts, R.5
Leunissen, J.A.6
|