A rapid and efficient platelet purification protocol for platelet gene expression studies

Methods in Molecular Biology

Volumn 788, Issue , 2012, Pages 155-172

  Amisten, Stefan ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

Gene expression; Leukocyte depletion; Microarray; Platelet activation; Platelet purification; qPCR; Real time PCR; RNA extraction

Indexed keywords

MESSENGER RNA;

ARTICLE; CELL SEPARATION; CYTOLOGY; DNA MICROARRAY; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; REAL TIME POLYMERASE CHAIN REACTION; THROMBOCYTE; THROMBOCYTE ACTIVATION; TIME;

BLOOD PLATELETS; CELL SEPARATION; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; HUMANS; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; PLATELET ACTIVATION; REAL-TIME POLYMERASE CHAIN REACTION; RNA, MESSENGER; TIME FACTORS;

EID: 84555191630     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-307-3_12     Document Type: Article

References (14)

1. Amisten S, Braun OO, Bengtsson A, Erlinge D. Gene expression profiling for the identification of G-protein coupled receptors in human platelets. Thromb Res. 2008;122(1):47-57. (Pubitemid 351601841)
2. Bugert P, Dugrillon A, Gunaydin A, Eichler H, Kluter H. Messenger RNA profiling of human platelets by microarray hybridization. Thromb Haemost. 2003 Oct;90(4):738-48. (Pubitemid 37287644)
3. Gnatenko DV, Dunn JJ, McCorkle SR, Weissmann D, Perrotta PL, Bahou WF. Transcript profiling of human platelets using microarray and serial analysis of gene expression. Blood. 2003 Mar 15;101(6):2285-93. (Pubitemid 36302071)
4. McRedmond JP, Park SD, Reilly DF, Coppinger JA, Maguire PB, Shields DC, et al. Integration of proteomics and genomics in platelets: a profile of platelet proteins and platelet-specific genes. Mol Cell Proteomics. 2004 Feb;3(2): 133-44. (Pubitemid 38714271)
5. Rox JM, Bugert P, Muller J, Schorr A, Hanfland P, Madlener K, et al. Gene expression analysis in platelets from a single donor: evaluation of a PCR-based amplification technique. Clin Chem. 2004 Dec;50(12):2271-8. (Pubitemid 39552161)
6. Rolf N, Knoefler R, Suttorp M, Kluter H, Bugert P. Optimized procedure for platelet RNA profiling from blood samples with limited platelet numbers. Clin Chem. 2005 Jun;51(6): 1078-80. (Pubitemid 40740909)
7. Available from: http://www.pall.com/medical-48187.asp .
8. RNeasy MinElute Cleanup Handbook2007.
9. Kendziorski C, Irizarry RA, Chen KS, Haag JD, Gould MN. On the utility of pooling biological samples in microarray experiments. Proc Natl Acad Sci USA. 2005 Mar 22;102(12): 4252-7. (Pubitemid 40397098)
10. Kendziorski CM, Zhang Y, Lan H, Attie AD. The efficiency of pooling mRNA in microarray experiments. Biostatistics. 2003 Jul;4(3):465-77.
11. Mary-Huard T, Daudin JJ, Baccini M, Biggeri A, Bar-Hen A. Biases induced by pooling samples in microarray experiments. Bioinformatics. 2007 Jul 1;23(13):i313-8. (Pubitemid 47244415)
12. Zhang W, Carriquiry A, Nettleton D, Dekkers JC. Pooling mRNA in microarray experiments and its effect on power. Bioinformatics. 2007 May 15;23(10):1217-24. (Pubitemid 47050537)
13. Wilson CL, Pepper SD, Hey Y, Miller CJ. Amplification protocols introduce systematic but reproducible errors into gene expression studies. Biotechniques. 2004 Mar;36(3):498-506. (Pubitemid 38299065)
14. McCloskey C, Jones S, Amisten S, Snowden RT, Kaczmarek LK, Erlinge D, et al. Kv1.3 is the exclusive voltage-gated K + channel of platelets and megakaryocytes: roles in membrane potential, Ca2+ signalling and platelet count. J Physiol. 2010 May 1;588(Pt 9):1399-406.