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Volumn 2, Issue , 2009, Pages

Validation of a primer optimisation matrix to improve the performance of reverse transcription - Quantitative real-time PCR assays

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EID: 76449109526     PISSN: None     EISSN: 17560500     Source Type: Journal    
DOI: 10.1186/1756-0500-2-112     Document Type: Article
Times cited : (31)

References (14)
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  • 2
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    • Quantification of mRNA using real-time RT-PCR
    • 10.1038/nprot.2006.236. 17406449
    • Quantification of mRNA using real-time RT-PCR. T Nolan RE Hands SA Bustin, Nat Protoc 2006 1 3 1559 1582 10.1038/nprot.2006.236 17406449
    • (2006) Nat Protoc , vol.1 , Issue.3 , pp. 1559-1582
    • Nolan, T.1    Hands, R.E.2    Bustin, S.A.3
  • 3
    • 0031608937 scopus 로고    scopus 로고
    • An introduction to PCR primer design and optimization of amplification reactions
    • 9664560
    • An introduction to PCR primer design and optimization of amplification reactions. JM Robertson J Walsh-Weller, Methods Mol Biol 1998 98 121 154 9664560
    • (1998) Methods Mol Biol , vol.98 , pp. 121-154
    • Robertson, J.M.1    Walsh-Weller, J.2
  • 4
    • 0034771194 scopus 로고    scopus 로고
    • Oligonucleotide melting temperatures under PCR conditions: Nearest-neighbor corrections for Mg(2+), deoxynucleotide triphosphate, and dimethyl sulfoxide concentrations with comparison to alternative empirical formulas
    • 11673362
    • Oligonucleotide melting temperatures under PCR conditions: nearest-neighbor corrections for Mg(2+), deoxynucleotide triphosphate, and dimethyl sulfoxide concentrations with comparison to alternative empirical formulas. N von Ahsen CT Wittwer E Schutz, Clin Chem 2001 47 11 1956 1961 11673362
    • (2001) Clin Chem , vol.47 , Issue.11 , pp. 1956-1961
    • Von Ahsen, N.1    Wittwer, C.T.2    Schutz, E.3
  • 5
    • 0025037431 scopus 로고
    • Optimization of the annealing temperature for DNA amplification in vitro
    • 2243783. 10.1093/nar/18.21.6409
    • Optimization of the annealing temperature for DNA amplification in vitro. W Rychlik WJ Spencer RE Rhoads, Nucleic Acids Res 1990 18 21 6409 6412 2243783 10.1093/nar/18.21.6409
    • (1990) Nucleic Acids Res , vol.18 , Issue.21 , pp. 6409-6412
    • Rychlik, W.1    Spencer, W.J.2    Rhoads, R.E.3
  • 6
    • 14044265394 scopus 로고    scopus 로고
    • Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction
    • 15331581
    • Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction. SA Bustin T Nolan, J Biomol Tech 2004 15 3 155 166 15331581
    • (2004) J Biomol Tech , vol.15 , Issue.3 , pp. 155-166
    • Bustin, S.A.1    Nolan, T.2
  • 7
    • 0036278859 scopus 로고    scopus 로고
    • Gene quantification using real-time quantitative PCR: An emerging technology hits the mainstream
    • 10.1016/S0301-472X(02)00806-8. 12063017
    • Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstream. DG Ginzinger, Exp Hematol 2002 30 6 503 512 10.1016/S0301-472X(02)00806-8 12063017
    • (2002) Exp Hematol , vol.30 , Issue.6 , pp. 503-512
    • Ginzinger, D.G.1
  • 13
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    • Primer3 on the WWW for general users and for biologist programmers
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    • Primer3 on the WWW for general users and for biologist programmers. S Rozen H Skaletsky, Methods Mol Biol 2000 132 365 386 10547847
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    • Rozen, S.1    Skaletsky, H.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.