A single intersubunit salt bridge affects oligomerization and catalytic activity in a bacterial quinone reductase

FEBS Journal

Volumn 276, Issue 18, 2009, Pages 5263-5274

  Binter, Alexandra ^a   Staunig, Nicole ^b   Jelesarov, Ilian ^c   Lohner, Karl ^d   Palfey, Bruce A ^e   Deller, Sigrid ^a   Gruber, Karl ^b   MacHeroux, Peter ^a  

^a GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

^b UNIVERSITY OF GRAZ   (Austria)

^c UNIVERSITY OF ZURICH   (Switzerland)

^d ERICH SCHMID INSTITUTE OF MATERIALS SCIENCE   (Austria)

^e UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

Author keywords

NADPH:FMN oxidoreductase; Oligomerization; Quinone reductase; Salt bridge; Thermostability

Indexed keywords

ASPARTIC ACID; DIMER; FLAVINE MONONUCLEOTIDE; GLYCINE; LEUCINE; LYSINE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE (PHOSPHATE) DEHYDROGENASE (QUINONE); REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; SODIUM CHLORIDE; TETRAMER;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMAL EXPERIMENT; ARTICLE; BACILLUS SUBTILIS; BACTERIUM; BINDING AFFINITY; CATALYSIS; CHEMICAL ANALYSIS; CONTROLLED STUDY; CRYSTAL STRUCTURE; CRYSTALLIZATION; DIMERIZATION; ENZYME ACTIVITY; EQUILIBRIUM CONSTANT; ESCHERICHIA COLI; ION PAIR EXTRACTION; NONHUMAN; OLIGOMERIZATION; OXIDATION; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN QUATERNARY STRUCTURE; PROTEIN STABILITY; PROTEIN VARIANT; STEADY STATE; WILD TYPE;

BACILLUS SUBTILIS; CATALYSIS; CRYSTALLIZATION; DIMERIZATION; ENZYME STABILITY; NAD(P)H DEHYDROGENASE (QUINONE); PROTEIN FOLDING; PROTEIN SUBUNITS;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS);

EID: 69449095076     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2009.07222.x     Document Type: Article

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