Improvement of α-amylase production by modulation of ribosomal component protein S12 in Bacillus subtilis 168

Applied and Environmental Microbiology

Volumn 72, Issue 1, 2006, Pages 71-77

  Kurosawa, Kazuhiko ^a   Hosaka, Takeshi ^a   Tamehiro, Norimasa ^a   Inaoka, Takashi ^a   Ochi, Kozo ^a  

^a NATIONAL FOOD RESEARCH INSTITUTE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTICS; BACTERIA; CELLS; ENZYMES; PROTEINS;

BACILLUS SUBTILIS 168; PROTEIN S12; STREPTOMYCES SPP;

BACTERIOLOGY;

AMYLASE; POLYURIDYLIC ACID; PROTEINASE; RIBOSOMAL PROTEIN S12; RIBOSOME PROTEIN; UNCLASSIFIED DRUG; PEPTIDE HYDROLASE;

ANTIBIOTICS; BACTERIOLOGY; CONCENTRATION (COMPOSITION); CYTOLOGY; ENZYME ACTIVITY; GENE EXPRESSION; MUTATION; PROTEIN;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL GENE; BACTERIAL STRAIN; CELL FREE SYSTEM; CELL GROWTH; ENZYME ACTIVITY; ENZYME SYNTHESIS; ESCHERICHIA COLI; IN VITRO STUDY; MUTANT; NONHUMAN; POINT MUTATION; RPSL GENE; STR GENE; STREPTOMYCES; BIOSYNTHESIS; ENZYMOLOGY; EVALUATION; GENE EXPRESSION REGULATION; GENETIC ENGINEERING; GENETICS; METABOLISM; METHODOLOGY; RIBOSOME;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; STREPTOMYCES;

ALPHA-AMYLASE; BACILLUS SUBTILIS; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC ENGINEERING; PEPTIDE HYDROLASES; POINT MUTATION; RIBOSOMAL PROTEINS; RIBOSOMES;

EID: 33644848909     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.72.1.71-77.2006     Document Type: Article

References (34)

1. Ayusawa, D., Y. Yoneda, K. Yamane, and B. Maruo. 1975. Pleiotropic phenomena in autolytic enzyme(s) content, flagellation, and simultaneous hyperproduction of extracellular α-amylase and protease in a Bacillus subtilis mutant. J. Bacteriol. 124:459-469.
2. Bertoldo, C., and G. Antranikian. 2002. Starch-hydrolyzing enzymes from thermophilic archaea and bacteria. Curr. Opin. Chem. Biol. 6:151-160.
3. Carter, A. P., W. M. Clemons, D. E. Brodersen, R. J. Morgan-Warren, B. T. Wimberly, and V. Ramakrishnan. 2000. Functional insights from the structure of the 30S ribosomal subunit and its interactions with antibiotics. Nature 407:340-348.
4. Cundliffe, E. 1990. Recognition sites for antibiotics within rRNA, p. 479-490. In A. D. W. E. Hill, R. A. Garrett, P. B. Moore, D. Schlessinger, and J. R. Warner (ed.). The ribosome: structure, function, and evolution. American Society for Microbiology, Washington, D. C.
5. Fujimoto, Z., K. Takase, N. Doui, M. Momma, T. Matsumoto, and H. Mizuno. 1998. Crystal structure of a catalytic-site mutant α-amylase from Bacillus subtilis complexed with maltopentaose. J. Mol. Biol. 277:393-407.
6. Fuwa, H. 1954. A new method for microdetermination of amylase activity by the use of amylose as the substrate. J. Biochem. (Tokyo) 41:603.
7. Hagihara, H., K. Igarashi, Y. Hayashi, K. Endo, K. Ikawa-Kitayama, K. Ozaki, S. Kawai, and S. Ito. 2001. Novel α-amylase that is highly resistant to chelating reagents and chemical oxidants from the alkaliphilic Bacillus isolate KSM-K38. Appl. Environ. Microbiol. 67:1744-1750.
8. Hesketh, A., and K. Ochi. 1997. A novel method for improving Streptomyces coelicolor A3(2) for production of actinorhodin by introduction of rpsL (encoding ribosomal protein S12) mutations conferring resistance to streptomycin. J. Antibiot. (Tokyo) 50:532-535.
9. Hosaka, T., N. Tamehiro, N. Chumpolkulwong, C. Hori-Takemoto, M. Shirouzu, S. Yokoyama, and K. Ochi. 2004. The novel mutation K87E in ribosomal protein S12 enhances protein synthesis activity during the late growth phase in Escherichia coli. Mol. Genet. Genomics 271:317-324.
10. Hosoya, Y., S. Okamoto, H. Muramatsu, and K. Ochi. 1998. Acquisition of certain streptomycin-resistant (str) mutations enhances antibiotic production in bacteria. Antimicrob. Agents Chemother. 42:2041-2047.
11. Hu, H., and K. Ochi. 2001. Novel approach for improving the productivity of antibiotic-producing strains by inducing combined resistant mutations. Appl. Environ. Microbiol. 67:1885-1892.
12. Hu, H., Q. Zhang, and K. Ochi. 2002. Activation of antibiotic biosynthesis by specified mutations in the rpoB gene (encoding the RNA polymerase β subunit) of Streptomyces lividans. J. Bacteriol. 184:3984-3991.
13. Ikawa, K., H. Araki, Y. Tsujino, Y. Hayashi, K. Igarashi, Y. Hatada, H. Hagihara, T. Ozawa, K. Ozaki, T. Kobayashi, and S. Ito. 1998. Hyperexpression of the gene for a Bacillus α-amylase in Bacillus subtilis cells: enzymatic properties and crystallization of the recombinant enzyme. Biosci. Biotechnol. Biochem. 62:1720-1725.
14. Inaoka, T., K. Kasai, and K. Ochi. 2001. Construction of an in vivo nonsense readthrough assay system and functional analysis of ribosomal proteins S12, S4, and S5 in Bacillus subtilis. J. Bacteriol. 183:4958-4963.
15. Kurland, C. G., F. Jorgensen, A. Richter, M. Ehrenberg, N. Bilgin, and A. M. Rojas. 1990. Through the accuracy window, p. 513-526. In W. E. Hill, A. E. Dahlberg, R. A. Garrett, P. B. Moore, D. Schlessinger, and J. R. Warner (ed.), The ribosome; structure, function, and evolution. American Society for Microbiology, Washington, D. C.
16. Legault-Demare, L., and G. H. Chambliss. 1974. Natural messenger ribonucleic acid-directed cell-free protein-synthesizing system of Bacillus subtilis. J. Bacteriol. 120:1300-1307.
17. Lodmell, J. S., and A. E. Dahlberg. 1997. A conformational switch in Escherichia coli 16S ribosomal RNA during decoding of messenger RNA. Science 277:1262-1267.
18. Machius, M., G. Wiegand, and R. Huber. 1995. Crystal structure of calcium-depleted Bacillus licheniformis α-amylase at 2.2 Å resolution. J. Mol. Biol. 246:545-559.
19. Nielsen, J. E., and T. V. Borchert. 2000. Protein engineering of bacterial α-amylases. Biochim. Biophys. Acta 1543:253-274.
20. Ochi, K., S. Okamoto, Y. Tozawa, T. Inaoka, T. Hosaka, J. Xu, and K. Kurosawa. 2004. Ribosome engineering and secondary metabolite production. Adv. Appl. Microbiol. 56:155-184.
21. Ofverstedt, L. G., K. Zhang, S. Tapio, U. Skoglund, and L. A. Isaksson. 1994. Starvation in vivo for aminoacyl-tRNA increases the spatial separation between the two ribosomal subunits. Cell 79:629-638.
22. Okamoto-Hosoya, Y., T. Hosaka, and K. Ochi. 2003. An aberrant protein synthesis activity is linked with antibiotic overproduction in rpsL mutants of Streptomyces coelicolor A3(2). Microbiology 149:3299-3309.
23. Pandey, A., P. Nigam, C. R. Soccol, V. T. Soccol, D. Singh, and R. Mohan. 2000. Advances in microbial amylases. Biotechnol. Appl. Biochem. 31:135-152.
24. Ramakrishnan, V. 2002. Ribosome structure and the mechanism of translation. Cell 108:557-572.
25. Sells, B. H., and H. L. Ennis. 1970. Polysome stability in relaxed and stringent strain of Escherichia coli during amino acid starvation. J. Bacteriol. 102:666-671.
26. Shima, J., A. Hesketh, S. Okamoto, S. Kawamoto, and K. Ochi. 1996. Induction of actinorhodin production by rpsL (encoding ribosomal protein S12) mutations that confer streptomycin resistance in Streptomyces lividans and Streptomyces coelicolor A3(2). J. Bacteriol. 178:7276-7284.
27. Shimizu, Y., T. Nishino, and S. Murao. 1984. Control of protease activity during sporulation of Bacillus subtilis. Agric. Biol. Chem. 48:3109-3114.
28. Sorensen, M. A., K. F. Jensen, and S. Pedersen. 1994. High concentrations of ppGpp decrease the RNA chain growth rate: implications for protein synthesis and translational fidelity during amino acid starvation in Escherichia coli. J. Mol. Biol. 236:441-454.
29. Tamehiro, N., T. Hosaka, J. Xu, H. Hu, N. Otake, and K. Ochi. 2003. Innovative approach for improvement of an antibiotic-overproducing industrial strain of Streptomyces albus. Appl. Environ. Microbiol. 69:6412-6417.
30. Timms, A. R., H. Steingrimsdottir, A. R. Lehmann, and B. A. Bridges. 1992. Mutant sequences in the rpsL gene of Escherichia coli B/r: mechanistic implications for spontaneous and ultraviolet light mutagenesis. Mol. Gen. Genet. 232:89-96.
31. Wallace, B. J., P.-C. Tai, and B. D. Davies. 1979. Streptomycin and related antibiotics, p. 272-303. In F. E. Hahn (ed.), Antibiotics. V. Mechanism of action of antibacterial agents. Springer-Verlag, New York, N.Y.
32. Yoneda, Y., and B. Maruo. 1975. Mutation of Bacillus subtilis causing hyperproduction of α-amylase and protease, and its synergistic effect. J. Bacteriol. 124:48-54.
33. Yusupov, M. M., G. Z. Yusupova, A. Baucom, K. Lieberman, T. N. Earnest, J. H. Cate, and H. F. Noller. 2001. Crystal structure of the ribosome at 5.5 Å resolution. Science 292:883-896.
34. Zhang, K., L. Pettersson-Landen, M. G. Fredriksson, L. G. Ofverstedt, U. Skoglund, and L. A. Isaksson. 1998. Visualization of a large conformation change of ribosomes in Escherichia coli cells starved for tryptophan or treated with kirromycin. Exp. Cell Res. 238:335-344.