Apoe, Mbl2, and Psp plasma protein levels correlate with diabetic phenotype in NZO mice - An optimized rapid workflow for SRM-based quantification

Journal of Proteome Research

Volumn 12, Issue 3, 2013, Pages 1331-1343

  Von Toerne, Christine ^a   Kahle, Melanie ^a   Schäfer, Alexander ^a   Ispiryan, Ruben ^a   Blindert, Marcel ^a   Hrabe De Angelis, Martin ^a   Neschen, Susanne ^a   Ueffing, Marius ^a,b   Hauck, Stefanie M ^a  

^a INSTITUTE OF EPIDEMIOLOGY   (Germany)

^b UNIVERSITY HOSPITAL TÜBINGEN   (Germany)

Author keywords

absolute quantification; AQUA peptides; depletion; label free quantification; New Zealand Obese mouse; protein signatures; ProteoMiner; selected reaction monitoring; type 2 diabetes

Indexed keywords

APOLIPOPROTEIN E; MANNOSE BINDING LECTIN 2; PLASMA PROTEIN; PSP PROTEIN; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BIOTECHNOLOGY; BLOOD SAMPLING; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; LIMIT OF QUANTITATION; LIQUID CHROMATOGRAPHY; LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY; MALE; MOUSE; NON INSULIN DEPENDENT DIABETES MELLITUS; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BLOOD LEVEL; PROTEIN DEPLETION; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEOMICS; REACTOR MONITORING; REPRODUCIBILITY; SELECTED REACTION MONITORING; TANDEM MASS SPECTROMETRY; WORKFLOW;

EID: 84874617282     PISSN: 15353893     EISSN: 15353907     Source Type: Journal    
DOI: 10.1021/pr3009836     Document Type: Article

References (69)

1. Hossain, P.; Kawar, B.; El Nahas, M. Obesity and diabetes in the developing world - a growing challenge N. Engl. J. Med. 2007, 356 (3) 213-215
2. Jurgens, H. S.; Neschen, S.; Ortmann, S.; Scherneck, S.; Schmolz, K.; Schuler, G.; Schmidt, S.; Bluher, M.; Klaus, S.; Perez-Tilve, D.; Tschop, M. H.; Schurmann, A.; Joost, H. G. Development of diabetes in obese, insulin-resistant mice: essential role of dietary carbohydrate in beta cell destruction Diabetologia 2007, 50 (7) 1481-1489
3. Poitout, V.; Robertson, R. P. Minireview: Secondary beta-cell failure in type 2 diabetes - a convergence of glucotoxicity and lipotoxicity Endocrinology 2002, 143 (2) 339-342
4. Whiteaker, J. R.; Lin, C.; Kennedy, J.; Hou, L.; Trute, M.; Sokal, I.; Yan, P.; Schoenherr, R. M.; Zhao, L.; Voytovich, U. J.; Kelly-Spratt, K. S.; Krasnoselsky, A.; Gafken, P. R.; Hogan, J. M.; Jones, L. A.; Wang, P.; Amon, L.; Chodosh, L. A.; Nelson, P. S.; McIntosh, M. W.; Kemp, C. J.; Paulovich, A. G. A targeted proteomics-based pipeline for verification of biomarkers in plasma Nat. Biotechnol. 2011, 29 (7) 625-634
5. Bielschowsky, F.; Bielschowsky, M. The New Zealand strain of obese mice; their response to stilboestrol and to insulin Aust. J. Exp. Biol. Med. Sci. 1956, 34 (3) 181-198
6. Crofford, O. B.; Davis, C. K., Jr. Growth characteristics, glucose tolerance and insulin sensitivity of New Zealand obese mice Metabolism 1965, 14, 271-280
7. Junger, E.; Herberg, L.; Jeruschke, K.; Leiter, E. H. The diabetes-prone NZO/Hl strain. II. Pancreatic immunopathology Lab Invest. 2002, 82 (7) 843-853
8. Lange, C.; Jeruschke, K.; Herberg, L.; Leiter, E. H.; Junger, E. The diabetes-prone NZO/Hl strain. Proliferation capacity of beta cells in hyperinsulinemia and hyperglycemia Arch. Physiol. Biochem. 2006, 112 (1) 49-58
9. Thorburn, A.; Andrikopoulos, S.; Proietto, J. Defects in liver and muscle glycogen metabolism in neonatal and adult New Zealand obese mice Metabolism 1995, 44 (10) 1298-1302
10. Andrikopoulos, S.; Proietto, J. The biochemical basis of increased hepatic glucose production in a mouse model of type 2 (non-insulin-dependent) diabetes mellitus Diabetologia 1995, 38 (12) 1389-1396
11. Ortlepp, J. R.; Kluge, R.; Giesen, K.; Plum, L.; Radke, P.; Hanrath, P.; Joost, H. G. A metabolic syndrome of hypertension, hyperinsulinaemia and hypercholesterolaemia in the New Zealand obese mouse Eur. J. Clin. Invest. 2000, 30 (3) 195-202
12. McInerney, M. F.; Najjar, S. M.; Brickley, D.; Lutzke, M.; Abou-Rjaily, G. A.; Reifsnyder, P.; Haskell, B. D.; Flurkey, K.; Zhang, Y. J.; Pietropaolo, S. L.; Pietropaolo, M.; Byers, J. P.; Leiter, E. H. Anti-insulin receptor autoantibodies are not required for type 2 diabetes pathogenesis in NZL/Lt mice, a New Zealand obese (NZO)-derived mouse strain Exp. Diabesity. Res. 2004, 5 (3) 177-185
13. Jurgens, H. S.; Schurmann, A.; Kluge, R.; Ortmann, S.; Klaus, S.; Joost, H. G.; Tschop, M. H. Hyperphagia, lower body temperature, and reduced running wheel activity precede development of morbid obesity in New Zealand obese mice Physiol. Genomics 2006, 25 (2) 234-241
14. Leiter, E. H. Selecting the ″right″ mouse model for metabolic syndrome and type 2 diabetes research Methods Mol. Biol. 2009, 560, 1-17
15. Kluge, R.; Giesen, K.; Bahrenberg, G.; Plum, L.; Ortlepp, J. R.; Joost, H. G. Quantitative trait loci for obesity and insulin resistance (Nob1, Nob2) and their interaction with the leptin receptor allele (LeprA720T/T1044I) in New Zealand obese mice Diabetologia 2000, 43 (12) 1565-1572
16. Reifsnyder, P. C.; Churchill, G.; Leiter, E. H. Maternal environment and genotype interact to establish diabesity in mice Genome Res. 2000, 10 (10) 1568-1578
17. Leiter, E. H.; Reifsnyder, P. C.; Flurkey, K.; Partke, H. J.; Junger, E.; Herberg, L. NIDDM genes in mice: deleterious synergism by both parental genomes contributes to diabetogenic thresholds Diabetes 1998, 47 (8) 1287-1295
18. Plum, L.; Giesen, K.; Kluge, R.; Junger, E.; Linnartz, K.; Schurmann, A.; Becker, W.; Joost, H. G. Characterisation of the mouse diabetes susceptibilty locus Nidd/SJL: islet cell destruction, interaction with the obesity QTL Nob1, and effect of dietary fat Diabetologia 2002, 45 (6) 823-830
19. Taylor, B. A.; Wnek, C.; Schroeder, D.; Phillips, S. J. Multiple obesity QTLs identified in an intercross between the NZO (New Zealand obese) and the SM (small) mouse strains Mamm. Genome 2001, 12 (2) 95-103
20. Anderson, N. L.; Anderson, N. G. The human plasma proteome: history, character, and diagnostic prospects Mol. Cell. Proteomics 2002, 1 (11) 845-867
21. Anderson, N. L. Counting the proteins in plasma Clin. Chem. 2010, 56 (11) 1775-1776
22. Boschetti, E.; Giorgio Righetti, P. Hexapeptide combinatorial ligand libraries: the march for the detection of the low-abundance proteome continues BioTechniques 2008, 44 (5) 663-665
23. Gerber, S. A.; Rush, J.; Stemman, O.; Kirschner, M. W.; Gygi, S. P. Absolute quantification of proteins and phosphoproteins from cell lysates by tandem MS Proc. Natl. Acad. Sci. U.S.A. 2003, 100 (12) 6940-6945
24. Fuchs, H.; Gailus-Durner, V.; Adler, T.; Aguilar-Pimentel, J. A.; Becker, L.; Calzada-Wack, J.; Da Silva-Buttkus, P.; Neff, F.; Gotz, A.; Hans, W.; Holter, S. M.; Horsch, M.; Kastenmuller, G.; Kemter, E.; Lengger, C.; Maier, H.; Matloka, M.; Moller, G.; Naton, B.; Prehn, C.; Puk, O.; Racz, I.; Rathkolb, B.; Romisch-Margl, W.; Rozman, J.; Wang-Sattler, R.; Schrewe, A.; Stoger, C.; Tost, M.; Adamski, J.; Aigner, B.; Beckers, J.; Behrendt, H.; Busch, D. H.; Esposito, I.; Graw, J.; Illig, T.; Ivandic, B.; Klingenspor, M.; Klopstock, T.; Kremmer, E.; Mempel, M.; Neschen, S.; Ollert, M.; Schulz, H.; Suhre, K.; Wolf, E.; Wurst, W.; Zimmer, A.; Hrabe de Angelis, M. Mouse phenotyping Methods 2010, 53 (2) 120-135
25. Gailus-Durner, V.; Fuchs, H.; Becker, L.; Bolle, I.; Brielmeier, M.; Calzada-Wack, J.; Elvert, R.; Ehrhardt, N.; Dalke, C.; Franz, T. J.; Grundner-Culemann, E.; Hammelbacher, S.; Holter, S. M.; Holzlwimmer, G.; Horsch, M.; Javaheri, A.; Kalaydjiev, S. V.; Klempt, M.; Kling, E.; Kunder, S.; Lengger, C.; Lisse, T.; Mijalski, T.; Naton, B.; Pedersen, V.; Prehn, C.; Przemeck, G.; Racz, I.; Reinhard, C.; Reitmeir, P.; Schneider, I.; Schrewe, A.; Steinkamp, R.; Zybill, C.; Adamski, J.; Beckers, J.; Behrendt, H.; Favor, J.; Graw, J.; Heldmaier, G.; Hofler, H.; Ivandic, B.; Katus, H.; Kirchhof, P.; Klingenspor, M.; Klopstock, T.; Lengeling, A.; Muller, W.; Ohl, F.; Ollert, M.; Quintanilla-Martinez, L.; Schmidt, J.; Schulz, H.; Wolf, E.; Wurst, W.; Zimmer, A.; Busch, D. H.; de Angelis, M. H. Introducing the German Mouse Clinic: open access platform for standardized phenotyping Nat. Methods 2005, 2 (6) 403-404
26. Hauck, S. M.; Dietter, J.; Kramer, R. L.; Hofmaier, F.; Zipplies, J. K.; Amann, B.; Feuchtinger, A.; Deeg, C. A.; Ueffing, M. Deciphering membrane-associated molecular processes in target tissue of autoimmune uveitis by label-free quantitative mass spectrometry Mol. Cell. Proteomics 2010, 9 (10) 2292-2305
27. Keller, A.; Nesvizhskii, A. I.; Kolker, E.; Aebersold, R. Empirical statistical model to estimate the accuracy of peptide identifications made by MS/MS and database search Anal. Chem. 2002, 74 (20) 5383-5392
28. Kuzyk, M. A.; Smith, D.; Yang, J.; Cross, T. J.; Jackson, A. M.; Hardie, D. B.; Anderson, N. L.; Borchers, C. H. Multiple reaction monitoring-based, multiplexed, absolute quantitation of 45 proteins in human plasma Mol. Cell. Proteomics 2009, 8 (8) 1860-1877
29. Boschetti, E.; Righetti, P. G. The art of observing rare protein species in proteomes with peptide ligand libraries Proteomics 2009, 9 (6) 1492-1510
30. Righetti, P. G.; Boschetti, E.; Zanella, A.; Fasoli, E.; Citterio, A. Plucking, pillaging and plundering proteomes with combinatorial peptide ligand libraries J. Chromatogr. A 2010, 1217 (6) 893-900
31. Mouton-Barbosa, E.; Roux-Dalvai, F.; Bouyssie, D.; Berger, F.; Schmidt, E.; Righetti, P. G.; Guerrier, L.; Boschetti, E.; Burlet-Schiltz, O.; Monsarrat, B.; Gonzalez de Peredo, A. In depth exploration of cerebrospinal fluid by combining peptide ligand library treatment and label free protein quantification Mol. Cell. Proteomics 2010, 9 (5) 1006-1021
32. Merl, J.; Ueffing, M.; Hauck, S. M.; von Toerne, C. Direct comparison of MS-based label-free and SILAC quantitative proteome profiling strategies in primary retinal Muller cells Proteomics 2012, 12 (12) 1902-1911
33. Addona, T. A.; Abbatiello, S. E.; Schilling, B.; Skates, S. J.; Mani, D. R.; Bunk, D. M.; Spiegelman, C. H.; Zimmerman, L. J.; Ham, A. J.; Keshishian, H.; Hall, S. C.; Allen, S.; Blackman, R. K.; Borchers, C. H.; Buck, C.; Cardasis, H. L.; Cusack, M. P.; Dodder, N. G.; Gibson, B. W.; Held, J. M.; Hiltke, T.; Jackson, A.; Johansen, E. B.; Kinsinger, C. R.; Li, J.; Mesri, M.; Neubert, T. A.; Niles, R. K.; Pulsipher, T. C.; Ransohoff, D.; Rodriguez, H.; Rudnick, P. A.; Smith, D.; Tabb, D. L.; Tegeler, T. J.; Variyath, A. M.; Vega-Montoto, L. J.; Wahlander, A.; Waldemarson, S.; Wang, M.; Whiteaker, J. R.; Zhao, L.; Anderson, N. L.; Fisher, S. J.; Liebler, D. C.; Paulovich, A. G.; Regnier, F. E.; Tempst, P.; Carr, S. A. Multi-site assessment of the precision and reproducibility of multiple reaction monitoring-based measurements of proteins in plasma Nat. Biotechnol. 2009, 27 (7) 633-641
34. Surinova, S.; Schiess, R.; Huttenhain, R.; Cerciello, F.; Wollscheid, B.; Aebersold, R. On the development of plasma protein biomarkers J. Proteome Res. 2012, 10 (1) 5-16
35. Fonslow, B. R.; Carvalho, P. C.; Academia, K.; Freeby, S.; Xu, T.; Nakorchevsky, A.; Paulus, A.; Yates, J. R., 3rd Improvements in proteomic metrics of low abundance proteins through proteome equalization using ProteoMiner prior to MudPIT J. Proteome Res. 2011, 10 (8) 3690-700
36. Addona, T. A.; Shi, X.; Keshishian, H.; Mani, D. R.; Burgess, M.; Gillette, M. A.; Clauser, K. R.; Shen, D.; Lewis, G. D.; Farrell, L. A.; Fifer, M. A.; Sabatine, M. S.; Gerszten, R. E.; Carr, S. A. A pipeline that integrates the discovery and verification of plasma protein biomarkers reveals candidate markers for cardiovascular disease Nat. Biotechnol. 2011, 29 (7) 635-643
37. Harkins, J. B. t.; Katz, B. B.; Pastor, S. J.; Osucha, P.; Hafeman, D. G.; Witkowski, C. E., 2nd; Norris, J. L. Parallel electrophoretic depletion, fractionation, concentration, and desalting of 96 complex biological samples for mass spectrometry Anal. Chem. 2008, 80 (8) 2734-2743
38. de Roos, B.; Duthie, S. J.; Polley, A. C.; Mulholland, F.; Bouwman, F. G.; Heim, C.; Rucklidge, G. J.; Johnson, I. T.; Mariman, E. C.; Daniel, H.; Elliott, R. M. Proteomic methodological recommendations for studies involving human plasma, platelets, and peripheral blood mononuclear cells J. Proteome Res. 2008, 7 (6) 2280-2290
39. Polaskova, V.; Kapur, A.; Khan, A.; Molloy, M. P.; Baker, M. S. High-abundance protein depletion: comparison of methods for human plasma biomarker discovery Electrophoresis 2010, 31 (3) 471-482
40. Yadav, A. K.; Bhardwaj, G.; Basak, T.; Kumar, D.; Ahmad, S.; Priyadarshini, R.; Singh, A. K.; Dash, D.; Sengupta, S. A systematic analysis of eluted fraction of plasma post immunoaffinity depletion: implications in biomarker discovery PLoS One 2011, 6 (9) e24442
41. Stempfer, R.; Kubicek, M.; Lang, I. M.; Christa, N.; Gerner, C. Quantitative assessment of human serum high-abundance protein depletion Electrophoresis 2008, 29 (21) 4316-4323
42. Gong, Y.; Li, X.; Yang, B.; Ying, W.; Li, D.; Zhang, Y.; Dai, S.; Cai, Y.; Wang, J.; He, F.; Qian, X. Different immunoaffinity fractionation strategies to characterize the human plasma proteome J. Proteome Res. 2006, 5 (6) 1379-1387
43. Liu, T.; Qian, W. J.; Mottaz, H. M.; Gritsenko, M. A.; Norbeck, A. D.; Moore, R. J.; Purvine, S. O.; Camp, D. G., 2nd; Smith, R. D. Evaluation of multiprotein immunoaffinity subtraction for plasma proteomics and candidate biomarker discovery using mass spectrometry Mol. Cell. Proteomics 2006, 5 (11) 2167-2174
44. Suhre, K.; Shin, S. Y.; Petersen, A. K.; Mohney, R. P.; Meredith, D.; Wagele, B.; Altmaier, E.; Deloukas, P.; Erdmann, J.; Grundberg, E.; Hammond, C. J.; de Angelis, M. H.; Kastenmuller, G.; Kottgen, A.; Kronenberg, F.; Mangino, M.; Meisinger, C.; Meitinger, T.; Mewes, H. W.; Milburn, M. V.; Prehn, C.; Raffler, J.; Ried, J. S.; Romisch-Margl, W.; Samani, N. J.; Small, K. S.; Wichmann, H. E.; Zhai, G.; Illig, T.; Spector, T. D.; Adamski, J.; Soranzo, N.; Gieger, C. Human metabolic individuality in biomedical and pharmaceutical research Nature 2011, 477 (7362) 54-60
45. Breslow, J. L. Transgenic mouse models of lipoprotein metabolism and atherosclerosis Proc. Natl. Acad. Sci. U.S.A. 1993, 90 (18) 8314-8318
46. Plump, A. S.; Smith, J. D.; Hayek, T.; Aalto-Setala, K.; Walsh, A.; Verstuyft, J. G.; Rubin, E. M.; Breslow, J. L. Severe hypercholesterolemia and atherosclerosis in apolipoprotein E-deficient mice created by homologous recombination in ES cells Cell 1992, 71 (2) 343-353
47. Zhang, S. H.; Reddick, R. L.; Piedrahita, J. A.; Maeda, N. Spontaneous hypercholesterolemia and arterial lesions in mice lacking apolipoprotein E Science 1992, 258 (5081) 468-471
48. Mahley, R. W. Apolipoprotein E: cholesterol transport protein with expanding role in cell biology Science 1988, 240 (4852) 622-630
49. Osada, J.; Joven, J.; Maeda, N. The value of apolipoprotein E knockout mice for studying the effects of dietary fat and cholesterol on atherogenesis Curr. Opin. Lipidol. 2000, 11 (1) 25-29
50. Meir, K. S.; Leitersdorf, E. Atherosclerosis in the apolipoprotein-E- deficient mouse: a decade of progress Arterioscler., Thromb., Vasc Biol 2004, 24 (6) 1006-1014
51. Kolovou, G.; Anagnostopoulou, K.; Mikhailidis, D. P.; Cokkinos, D. V. Apolipoprotein E knockout models Curr. Pharm. Des. 2008, 14 (4) 338-351
52. King, V. L.; Hatch, N. W.; Chan, H. W.; de Beer, M. C.; de Beer, F. C.; Tannock, L. R. A murine model of obesity with accelerated atherosclerosis Obesity (Silver Spring) 2010, 18 (1) 35-41
53. Fan, P.; Liu, H.; Wang, Y.; Zhang, F.; Bai, H. Apolipoprotein E-containing HDL-associated platelet-activating factor acetylhydrolase activities and malondialdehyde concentrations in patients with PCOS Reprod. BioMed. Online 2012, 24 (2) 197-205
54. Wallis, R.; Mitchell, D. A.; Schmid, R.; Schwaeble, W. J.; Keeble, A. H. Paths reunited: Initiation of the classical and lectin pathways of complement activation Immunobiology 2010, 215 (1) 1-11
55. Osthoff, M.; Trendelenburg, G.; Eisen, D. P.; Trendelenburg, M. Mannose-binding lectin-the forgotten molecule? Nat. Med. 2011, 17 (12) 1547-1548; author reply 1548
56. Ezekowitz, R. A.; Sastry, K.; Bailly, P.; Warner, A. Molecular characterization of the human macrophage mannose receptor: demonstration of multiple carbohydrate recognition-like domains and phagocytosis of yeasts in Cos-1 cells J. Exp. Med. 1990, 172 (6) 1785-1794
57. Eisen, D. P. Mannose-binding lectin deficiency and respiratory tract infection J. Innate Immun. 2010, 2 (2) 114-122
58. Ogden, C. A.; deCathelineau, A.; Hoffmann, P. R.; Bratton, D.; Ghebrehiwet, B.; Fadok, V. A.; Henson, P. M. C1q and mannose binding lectin engagement of cell surface calreticulin and CD91 initiates macropinocytosis and uptake of apoptotic cells J. Exp. Med. 2001, 194 (6) 781-795
59. Cervera, A.; Planas, A. M.; Justicia, C.; Urra, X.; Jensenius, J. C.; Torres, F.; Lozano, F.; Chamorro, A. Genetically-defined deficiency of mannose-binding lectin is associated with protection after experimental stroke in mice and outcome in human stroke PLoS One 2010, 5 (2) e8433
60. Osthoff, M.; Katan, M.; Fluri, F.; Schuetz, P.; Bingisser, R.; Kappos, L.; Steck, A. J.; Engelter, S. T.; Mueller, B.; Christ-Crain, M.; Trendelenburg, M. Mannose-binding lectin deficiency is associated with smaller infarction size and favorable outcome in ischemic stroke patients PLoS One 2011, 6 (6) e21338
61. Garred, P.; Larsen, F.; Seyfarth, J.; Fujita, R.; Madsen, H. O. Mannose-binding lectin and its genetic variants Genes Immun. 2006, 7 (2) 85-94
62. Ezekowitz, R. A. Genetic heterogeneity of mannose-binding proteins: the Jekyll and Hyde of innate immunity? Am. J. Hum. Genet. 1998, 62 (1) 6-9
63. Fortpied, J.; Vertommen, D.; Van Schaftingen, E. Binding of mannose-binding lectin to fructosamines: a potential link between hyperglycaemia and complement activation in diabetes Diabetes Metab. Res. Rev. 2010, 26 (4) 254-260
64. Abdolhosseini, M.; Sotsky, J. B.; Shelar, A. P.; Joyce, P. B.; Gorr, S. U. Human parotid secretory protein is a lipopolysaccharide-binding protein: identification of an anti-inflammatory peptide domain Mol. Cell. Biochem. 2012, 359 (1-2) 1-8
65. Mednieks, M. I.; Szczepanski, A.; Clark, B.; Hand, A. R. Protein expression in salivary glands of rats with streptozotocin diabetes Int. J. Exp. Pathol. 2009, 90 (4) 412-422
66. Geetha, C.; Venkatesh, S. G.; Bingle, L.; Bingle, C. D.; Gorr, S. U. Design and validation of anti-inflammatory peptides from human parotid secretory protein J. Dent. Res. 2005, 84 (2) 149-153
67. Border, M. B.; Schwartz, S.; Carlson, J.; Dibble, C. F.; Kohltfarber, H.; Offenbacher, S.; Buse, J. B.; Bencharit, S. Exploring salivary proteomes in edentulous patients with type 2 diabetes Mol. Biosyst. 2012, 8 (4) 1304-1310
68. Khovidhunkit, W.; Hachem, J. P.; Medzihradszky, K. F.; Duchateau, P. N.; Shigenaga, J. K.; Moser, A. H.; Movsesyan, I.; Naya-Vigne, J.; Kane, J. P.; Feingold, K. R.; Grunfeld, C. Parotid secretory protein is an HDL-associated protein with anticandidal activity Am. J. Physiol. Regul. Integr. Comp. Physiol. 2005, 288 (5) R1306-1315
69. Gautier, T.; Lagrost, L. Plasma PLTP (phospholipid-transfer protein): an emerging role in 'reverse lipopolysaccharide transport' and innate immunity Biochem. Soc. Trans. 2011, 39 (4) 984-988