Molecular cloning, expression, purification, and functional characterization of dammarenediol synthase from panax ginseng

BioMed Research International

Volumn 2013, Issue , 2013, Pages

  Hu, Wei ^a   Liu, Ning ^a   Tian, Yuhua ^a   Zhang, Lianxue ^b  

^a JILIN AGRICULTURAL UNIVERSITY   (China)

^b CHANGCHUN UNIVERSITY OF CHINESE MEDICINE   (China)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; DAMMARENEDIOL SYNTHASE; GINSENOSIDE; PRIMER DNA; UNCLASSIFIED DRUG; VEGETABLE PROTEIN; DAMMARENEDIOL-II SYNTHASE, PANAX GINSENG; RECOMBINANT PROTEIN; TRANSFERASE; DAMMARENEDIOL II SYNTHASE, PANAX GINSENG;

AFFINITY CHROMATOGRAPHY; ARTICLE; BIOSYNTHESIS; CONTROLLED STUDY; DNA SEQUENCE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME SYNTHESIS; ESCHERICHIA COLI; GENE CONSTRUCT; GENE EXPRESSION; GENE LIBRARY; GINSENG; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PLANT GENE; PLANT ROOT; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN CONTENT; PROTEIN FUNCTION; CHEMISTRY; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; PANAX;

ALKYL AND ARYL TRANSFERASES; CLONING, MOLECULAR; GENE EXPRESSION; GENE LIBRARY; PANAX; PLANT PROTEINS; RECOMBINANT PROTEINS;

EID: 84874589873     PISSN: 23146133     EISSN: 23146141     Source Type: Journal    
DOI: 10.1155/2013/285740     Document Type: Article

References (23)

1. Qu D. F., Yu H. J., Liu Z., Zhang D. F., Zhou Q. J., Zhang H. L., Du A. F., Ginsenoside Rg1 enhances immune response induced by recombinant Toxoplasma gondii SAG1 antigen. Veterinary Parasitology 2011 179 1-3 28 34 2-s2.0-79957608931 10.1016/j.vetpar.2011.02.008
2. Zhao R., Zhang Z., Song Y., Wang D., Qi J., Wen S., Implication of phosphatidylinositol-3 kinase/Akt/glycogen synthase kinase-3 β pathway in ginsenoside Rb1's attenuation of beta-amyloid-induced neurotoxicity and tau phosphorylation. Journal of Ethnopharmacology 2011 133 3 1109 1116 2-s2.0-79551543865 10.1016/j.jep.2010.11.054
3. Kim Y. C., Kim S. R., Markelonis G. J., Oh T. H., Ginsenosides Rb1 and Rg3 protect cultured rat cortical cells from glutamate-induced neurodegeneration. Journal of Neuroscience Research 1998 53 4 426 432 10.1002/(SICI)1097-4547(19980815)53:4<426::AID-JNR4>3.0.CO;2-8 (Pubitemid 28363204)
4. Dang H., Chen Y., Liu X., Wang Q., Wang L., Jia W., Wang Y., Antidepressant effects of ginseng total saponins in the forced swimming test and chronic mild stress models of depression. Progress in Neuro-Psychopharmacology and Biological Psychiatry 2009 33 8 1417 1424 2-s2.0-71549173390 10.1016/j.pnpbp.2009.07.020
5. Liu J., Shimizu K., Yu H., Zhang C., Jin F., Kondo R., Stereospecificity of hydroxyl group at C-20 in antiproliferative action of ginsenoside Rh2 on prostate cancer cells. Fitoterapia 2010 81 7 902 905 2-s2.0-77956680962 10.1016/j.fitote.2010.05.020
6. Chen Z., Gang Z., Advances in the research of pharmacological effects of ginsenoside Rd. Chinese Journal of New Drugs 2011 20 11 953 958
7. Chun Xi H., Shou Jing Z., Yan Long L., Jian Hua W., Advances in genetic transformation of Panax ginseng. China Biotechnology 2009 29 9 91 96
8. Liang Y., Zhao S., Zhang X., Antisense suppression of cycloartenol synthase results in elevated ginsenoside levels in Panax ginseng hairy roots. Plant Molecular Biology Reporter 2009 27 3 298 304 2-s2.0-70350005063 10.1007/s11105-008-0087-7
9. Lee M. H., Jeong J. H., Seo J. W., Shin C. G., Kim Y. S., In J. G., Yang D. C., Yi J. S., Choi Y. E., Enhanced triterpene and phytosterol biosynthesis in Panax ginseng overexpressing squalene synthase gene. Plant and Cell Physiology 2004 45 8 976 984 2-s2.0-4644290998
10. Kim Y. S., Han J. Y., Lim S., Choi Y. E., Ginseng metabolic engineering: regulation of genes related to ginsenoside biosynthesis. Journal of Medicinal Plant Research 2009 3 13 1270 1276 2-s2.0-75649151509
11. Augustin J. M., Kuzina V., Andersen S. B., Bak S., Molecular activities, biosynthesis and evolution of triterpenoid saponins. Phytochemistry 2011 72 6 435 457 2-s2.0-79952900480 10.1016/j.phytochem.2011.01.015
12. Kushiro T., Ohno Y., Shibuya M., Ebizuka Y., In vitro conversion of 2,3-oxidosqualene into dammarenediol by Panax ginseng microsomes. Biological and Pharmaceutical Bulletin 1997 20 3 292 294 2-s2.0-0030964465 (Pubitemid 27148983)
13. Iturbe-Ormaetxe I., Haralampidis K., Papadopoulou K., Osbourn A. E., Molecular cloning and characterization of triterpene synthases from Medicago truncatula and Lotus japonicus. Plant Molecular Biology 2003 51 5 731 743 2-s2.0-0037356157 10.1023/A:1022519709298 (Pubitemid 36408925)
14. Hayashi H., Huang P., Kirakosyan A., Inoue K., Hiraoka N., Ikeshiro Y., Kushiro T., Shibuya M., Ebizuka Y., Cloning and characterization of a cDNA encoding β -amyrin synthase involved in glycyrrhizin and soyasaponin biosyntheses in licorice. Biological and Pharmaceutical Bulletin 2001 24 8 912 916 2-s2.0-0034862884 10.1248/bpb.24.912 (Pubitemid 32750317)
15. Morita M., Shibuya M., Kushiro T., Masuda K., Ebizuka Y., Molecular cloning and functional expression of triterpene synthases from pea (Pisum sativum): new α -amyrin-producing enzyme is a multifunctional triterpene synthase. European Journal of Biochemistry 2000 267 12 3453 3460 2-s2.0-0001031993 10.1046/j.1432-1327.2000.01357.x (Pubitemid 30423004)
16. Wellenreuther R., Schupp I., Poustka A., Wiemann S., SMART amplification combined with cDNA size fractionation in order to obtain large full-length clones. BMC Genomics 2004 5 1 36 2-s2.0-9444254040 10.1186/1471-2164-5-36 (Pubitemid 39565314)
17. Fu G. K., Stuve L. L., Improved method for the construction of full-length enriched cDNA libraries. BioTechniques 2003 34 5 954 957 2-s2.0-0037652314 (Pubitemid 36554563)
18. Tansakul P., Shibuya M., Kushiro T., Ebizuka Y., Dammarenediol-II synthase, the first dedicated enzyme for ginsenoside biosynthesis, in Panax ginseng. FEBS Letters 2006 580 22 5143 5149 2-s2.0-33748809553 10.1016/j.febslet.2006.08.044 (Pubitemid 44415784)
19. Dan H., Balachandran A., Lin M., A pair of ligation-independent escherichia coli expression vectors for rapid addition of a polyhistidine affinity tag to the N- or C-termini of recombinant proteins. Journal of Biomolecular Techniques 2009 20 5 241 248
20. Ke T., Liang S., Huang J., Mao H., Chen J., Dong C., Huang J., Liu S., Kang J., Liu D., Ma X., A novel PCR-based method for high throughput prokaryotic expression of antimicrobial peptide genes. BMC Biotechnology 2012 12, article 10
21. Vogeser M., Kirchhoff F., Progress in automation of LC-MS in laboratory medicine. Clinical Biochemistry 2011 44 1 4 13 2-s2.0-78650882617 10.1016/j.clinbiochem.2010.06.005
22. Preis A., Mugisha L., Hauser B., Weltring A., Deschner T., Androgen and androgen metabolite levels in serum and urine of East African chimpanzees (Pan troglodytes schweinfurthii): comparison of EIA and LC-MS analyses. General and Comparative Endocrinology 2011 174 3 335 343 10.1016/j.ygcen.2011.09.010
23. Han J. Y., Kwon Y. S., Yang D. C., Jung Y. R., Choi Y. E., Expression and RNA interference-induced silencing of the dammarenediol synthase gene in Panax ginseng. Plant and Cell Physiology 2006 47 12 1653 1662 2-s2.0-33845712221 10.1093/pcp/pcl032 (Pubitemid 44968260)