Ginseng metabolic engineering: Regulation of genes related to ginsenoside biosynthesis

Journal of Medicinal Plants Research

Volumn 3, Issue 13, 2009, Pages 1270-1276

  Kim, Yun Soo ^a   Han, Jung Yeon ^a   Lim, Soon ^a   Choi, Yong Eui ^a  

^a Kangwon National University   (South Korea)

Author keywords

Expressed sequence tags; Ginsenoside; Panax ginseng; Secondary metabolite; Triterpene

Indexed keywords

DAMMARENEDIOL SYNTHASE; GINSENOSIDE; GINSENOSIDE RB 1; GINSENOSIDE RB 2; GINSENOSIDE RC; GINSENOSIDE RD; GINSENOSIDE RE; GINSENOSIDE RF; GINSENOSIDE RG 1; GLYCOSYLTRANSFERASE; OXIDOSQUALENE CYCLASE; PHYTOSTEROL; PROTOPANAXADIOL; SAPONIN; SQUALENE MONOOXYGENASE; SQUALENE SYNTHASE; TRITERPENE; TRITERPENOID; UNCLASSIFIED DRUG; VEGETABLE PROTEIN;

BIOSYNTHESIS; DNA MODIFICATION; DRUG OXIDATION; EXPRESSED SEQUENCE TAG; GENE EXPRESSION REGULATION; GINSENG; GLYCOSYLATION; METABOLIC ENGINEERING; NONHUMAN; NUCLEOTIDE SEQUENCE; PLANT BIOTECHNOLOGY; PLANT GENE; REVIEW; UNINDEXED SEQUENCE; UPREGULATION;

PANAX GINSENG;

EID: 75649151509     PISSN: 19960875     EISSN: 19960875     Source Type: Journal    
DOI: None     Document Type: Review

References (46)

1. Abe I, Rohmer M, Prestwich GD (1993) Enzymatic cyclization of squalene and oxidosqualene to sterols and triterpenes. Chem. Rev. 93: 2189-2206.
2. Asaka I, Li I, Hirotani M, Asada Y, Furuya T (1993) Production of ginsenoside saponins by culturing ginseng (Panax ginseng) embryogenic tissues in bioreactors. Biotechnol. Lett. 15:1259-1264
3. Bae KH, Choi YE, Shin CG, Kim YY, Kim YS (2006) Enhanced ginsenoside productivity by combination of ethephon and methyl jasmonate in ginseng (Panax ginseng C.A. Meyer) adventitious root cultures. Biotechnol Lett. 28:1163-1166
4. Bang KH, Kim OT, Jung SJ, Kim YC, Park HW, Cha SW (2009) Molecular characterization of ginseng farnesyl diphosphate synthase gene and its up-regulation by methyl jasmonate. Biol. Plant (in press)
5. Briskin DP (2000) Medicinal plants and phytomedicines. Linking plant biochemistry and physiology to human health. Plant Physiol. 124:507-514
6. Choi DW, Jung JD, Ha YI, Park HW, In DS, Chung HJ, Liu JR (2005) Analysis of transcripts in methyl jasmonate-treated ginseng hairy roots to identify genes involved in the biosynthesis of ginsenosides and other secondary metabolites. Plant Cell. Rep. 23:557-566.
7. Choi SM, Son SH, Kwon OW, Seon JH, Paek KY (2000) Pilot-scale culture of adventitious roots of ginseng in a bioreactor system. Plant Cell Tiss. Organ Cult. 62:187-193
8. Coleman CI, Hebert JH, Reddy P (2003) The effects of Panax ginseng on quality of life. J. Clin. Pharm. Ther. 28:5-15
9. Cui JF, Eneroth P, Bruhn JC (1999) Gynostemma pentaphyllum: identification of major sapogenins and differentiation from Panax species. Eur. J. Pharm. Sci. 8:187-191
10. Dey L, Xie JT, Wang A, Wu J, Maleckar SA, Yuan CS (2003) Antihyperglycemic effects of ginseng: comparison between root and berry. Phytomedicine 10:600-605
11. Ellis JM, Reddy P (2002) Effects of Panax ginseng on quality of life. Ann. Pharmacther. 36:375-379
12. Furuya T, Ushiyama K (1994) Ginseng production in cultures of Panax ginseng cells. In: Shargool P, Ngo TT (eds) Biotechnological application of plant cultures. CRC, Boca Raton, pp 1-22
13. Han JY, Kwon YS, Yang DC, Jung YR, Choi YE (2006) Expression and RNA interference-induced silencing of the dammarenediol synthase gene in Panax ginseng. Plant Cell Physiol. 47:1653-1662
14. Han JY, In JG, Kwon YS, Choi YE (2010) Regulation of ginsenoside and phytosterol biosynthesis by RNA interferences of squalene epoxidase gene in Panax ginseng. Phytochemistry. 71:36-46.
15. Haralampidis K, Trojanowska M, Oshourn AE (2001) Biosynthesis of triterpenoid saponin in plants. Adv Biochem Eng Biotechnol 75:31-49
16. Harrison MD (1990) The biosynthesis of triterpenoids, steroids, and carotenoids. Nat. Prod. Rep. 7:459-484
17. Hu FX, Zhong JJ (2007) Role of jasmonic acid in alteration of ginsenoside heterogeneity in elicited cell cultures of Panax notoginseng. J. Biosci. Bioeng. 104:513-516
18. Jeong GT, Park DH, Hwang B, Woo JC (2003) Comparison of growth characteristics of Panax ginseng hairy roots in various bioreactors. Appl. Biochem. Biotechnol. 107:493-503
19. Jung JD, Park HW, Hahn Y, Hur CG, In DS, Chung HJ, Liu JR, Choi DW (2003) Discovery of genes for ginsenoside biosynthesis by analysis of ginseng expressed sequence tag. Plant Cell Rep. 22:224-230
20. Kevers C, Jacques P, Thonart P, Gaspar T (1999) In vitro root cultures of Panax ginseng and Panax quinquefolium. Plant Growth Regul. 27:173-178
21. Kiefer D, Pantuso T (2003) Panax ginseng. Am. Fam. Physician 68: 1539-1542.
22. Kim OT, Bang KH, Kim YC, Hyun DY, Kim MY, Cha SW (2009) Upregulation of ginsenoside and gene expression related to triterpene biosynthesis in ginseng hairy root cultures elicited by methyl jasmonate. Plant Cell Tiss. Organ Cult. 98:25-33
23. Kim YS, Hahn EJ, Murthy HN, Paek KY (2004) Adventitious root growth and ginsenoside accumulation in Panax ginseng cultures as affected by methyl jasmonate. Biotechnol. Lett. 26:1619-1622
24. Kim YS, Yeung EC, Hahn EJ, Paek KY (2007) Combined effects of phytohormone, indole-3-butyric acid, and methyl jasmonate on root growth and ginsenoside production in adventitious root cultures of Panax ginseng C.A. Meyer. Biotechnol. Lett. 29:1789-1792
25. Kushiro T, Ohno Y, Shibuya Y, Ebizuka Y (1997) In vitro conversion of 2,3-oxidosqualene into dammarenediol by Panax ginseng microsomes. Biol. Pharm. Bull. 20: 292-294.
26. Kushiro T, Shibuya Y, Ebizuka Y (1998) β-amyrin synthase: cloning of oxidosqualene cyclase that catalyze the formation of the most popular triterpene among higher plants. Eur. J. Biochem. 256:238-244.
27. Kuzuyama T (2002) Mevalonate and nonmevalonate pathways for the biosynthesis of isoprene units. Biosci. Biotechnol. Biochem. 66:1619-1627
28. Lee MH, Jeong JH, Seo JW, Shin CG, Kim YS, In JG, Yang DC, Yi JS, Choi YE (2004) Enhanced triterpene and phyotsterol biosynthesis in Panax ginseng overexpressing squalene synthase gene. Plant Cell Physiol. 45:976-984
29. Madey E, Nowack LM, Thompson JE (2002) Isolation and characterization of lipid in phloem sap of canola. Planta 214:625-634
30. Shibata S (2001) Chemistry and cancer preventing activities of ginseng saponins and some related triterpenoid compounds. J. Kor. Med. Sci. 16 [Suppl]:S28-S37
31. Shibuya M, Hoshino M, Katsube Y, Hayashi H, Kushiro T, Ebizuka Y (2006) Identification of β-amyrin and sophoradiol 24-hydroxylase by expressed sequence tag mining and functional expression assay. FEBS J. 273:948-959
32. Seo JW, Jeong JH, Shin CG, Lo SC, Han SS, Yu KW, Harada E, Han JY, Choi YE (2005) Overexpression of squalene synthase in Eleutherococcus senticosus increases phytosterol and triterpene accumulation. Phytochemistry 66:869-877
33. Son SH, Choi SM, Soo JH, Yun SR, Choi MS, Shin EM, Hong YP (1999) Induction and cultures of mountain ginseng adventitious roots and AFLP analysis for identifying mountain ginseng. Biotechnol. Bioprocess Eng. 4:119-123.
34. Sticher O (1998) Getting to the root of ginseng. Chemtech 28:26-32.
35. Suzuki M, Xiang T, Ohyama K, Seki H, Saito K, Muranaka T, Hayashi H, Katsube Y, Kushiro T, Shibuya M, Ebizuka Y (2006) Lanosterol synthase in dicotyledonous plants. Plant Cell Physiol 47:565-571.
36. Tansakul P, Shibuya M, Kushiro T, Ebizuka Y (2006). Dammarenediol-II synthase, the first dedicated enzyme for ginsenoside biosynthesis, in Panax ginseng. FEBS Lett. 580:5143-5149.
37. Uchida H, Sugiyama R, Nakayachi O, Takemura M, Ohyama K (2007) Expression of the gene for sterol-biosynthesis enzyme squalene epoxidase in parenchyma cells of the oil plant, Euphorbia tirucalli. Planta 226:1109-1115.
38. Vogler BK, Pittler MH, Ernst E (1999) The efficacy of ginseng. A systematic review of randomized clinical trials. Eur. J. Clin. Pharmacol. 55:567-575.
39. Wang W, Zhong JJ (2002) Manipulation of ginsenoside heterogeneity in cell cultures of Panax notoginseng by addition of jasmonates. J. Biosci. Bioeng. 93:48-53.
40. Wu JY, Zhong JJ (1999). Production of ginseng and its bioactive components in plant cell culture: current technological and applied aspects. J. Biotechnol. 68:89-99.
41. Yoshikawa T, Furuya T (1987). Saponin production by cultures of Panax ginseng transformed with Agrobacterium rhizogens. Plant Cell Rep. 6:449-453.
42. Yu KW, Gao WY, Son SH, Paek KY (2000). Improvement of ginsenoside production by jasmonic acid and some other elicitors in hairy root culture of Ginseng (Panax ginseng C.A. Meyer). In Vitro Cell Dev. Biol. -Plant 36:424-428.
43. Yu KW, Gao WY, Hahn EJ, Paek KY (2002). Jasmonic acid improves ginsenoside accumulation in adventitious root culture of Panax ginseng C.A. Meyer. Biochem. Eng. J. 3596:1-5.
44. Yue CJ, Zhong JJ (2005). Purification and characterization of UDPG:ginsenoside Rd glucosyltransferase from suspended cells of Panax notoginseng. Process Biochem. 40: 3742-3748.
45. Yue CJ, Zhou X, Zhong JJ (2008) Protopanaxadiol 6-hydroxylase and its role in regulating the ginsenoside heterogeneity in Panax notoginseng cells. Biotechnol. Bioeng. 100: 933-940.
46. Yun TK (2001) Panax ginseng-a non-organ-specific cancer preventive? Lancet Oncol. 2: 49-55.