A dual ELP-tagged split intein system for non-chromatographic recombinant protein purification

Applied Microbiology and Biotechnology

Volumn 97, Issue 2, 2013, Pages 829-835

  Shi, Changhua ^a,b   Meng, Qing ^a   Wood, David W ^b  

^a DONGHUA UNIVERSITY   (China)

^b The Ohio State University   (United States)

Author keywords

Elastin like protein; Protein purification; Self cleaving tag; Split intein

Indexed keywords

CELL LYSIS; DIFFERENT SIZES; ELASTIN-LIKE PROTEINS; IN-VITRO; INTEINS; PROTEIN EXPRESSIONS; PROTEIN PURIFICATION; PURIFICATION METHOD; REACTION MIXTURE; RECOMBINANT PROTEIN PURIFICATION; SELF-CLEAVAGE; SELF-CLEAVING TAG; SPLIT INTEIN; TARGET PROTEINS;

RECOMBINANT PROTEINS;

PURIFICATION;

BETA GALACTOSIDASE; BETA LACTAMASE; ELASTIN; GREEN FLUORESCENT PROTEIN; INTEIN; MALTOSE BINDING PROTEIN; RECOMBINANT PROTEIN;

CHROMATOGRAPHY; PRECIPITATION (CHEMISTRY); PROTEIN; RECOMBINATION;

ARTICLE; CONTROLLED STUDY; CYTOLYSIS; ESCHERICHIA COLI; IN VITRO STUDY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ASSEMBLY; PROTEIN CLEAVAGE; PROTEIN PURIFICATION;

BIOTECHNOLOGY; ELASTIN; ESCHERICHIA COLI; INTEINS; RECOMBINANT FUSION PROTEINS;

EID: 84873994701     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-012-4601-3     Document Type: Article

References (23)

1. Banki MR, Feng LA, Wood DW (2005) Simple bioseparations using self-cleaving elastin-like polypeptide tags. Nat Methods 2(9):659-661
2. Chong SR, Mersha FB, Comb DG, Scott ME, Landry D, Vence LM, Perler FB, Benner J, Kucera RB, Hirvonen CA, Pelletier JJ, Paulus H, Xu MQ (1997) Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element. Gene 192(2):271-281
3. Cui C, Zhao W, Chen J, Wang J, Li Q (2006) Elimination of in vivo cleavage between target protein and intein in the intein-mediated protein purification systems. Protein Expr Purif 50(1):74-81. doi: 10.1016/j.pep.2006. 05.019
4. de Moreno MR, Smith JF, Smith RV (1986) Mechanism studies of coomassie blue and silver staining of proteins. J Pharm Sci 75(9):907-911
5. Derbyshire V, Wood DW, Wu W, Dansereau JT, Dalgaard JZ, Belfort M (1997) Genetic definition of a protein-splicing domain: functional mini-inteins support structure predictions and a model for intein evolution. P Natl Acad Sci USA 94(21):11466-11471
6. Evans TC, Benner J, Xu MQ (1999) The in vitro ligation of bacterially expressed proteins using an intein from Methanobacterium thermoautotrophicum. J Biol Chem 274(7):3923-3926
7. Fong BA, Wu WY, Wood DW (2009) Optimization of ELP-intein mediated protein purification by salt substitution. Protein Expres Purif 66(2):198-202. doi: 10.1016/j.pep.2009.03.009
8. Ge X, Yang DSC, Trabbic-Carlson K, Kim B, Chilkoti A, Filipe CDM (2005) Self-cleavable stimulus responsive tags for protein purification without chromatography. J Am Chem Soc 127(32):11228-11229. doi: 10.1021/Ja0531125
9. Gogarten JP, Senejani AG, Zhaxybayeva O, Olendzenski L, Hilario E (2002) Inteins: structure, function, and evolution. Annu Rev Microbiol 56:263-287. doi: 10.1146/annurev.micro.56.012302.160741
10. Lu W, Sun ZY, Tang YC, Chen JY, Tang FY, Zhang J, Liu JN (2011) Split intein facilitated tag affinity purification for recombinant proteins with controllable tag removal by inducible auto-cleavage. J Chromatogr A 1218(18):2553-2560. doi: 10.1016/j.chroma.2011.02.053
11. Mathys S, Evans TC, Chute IC, Wu H, Chong SR, Benner J, Liu XQ, Xu MQ (1999) Characterization of a self-splicing mini-intein and its conversion into autocatalytic N- and C-terminal cleavage elements: facile production of protein building blocks for protein ligation. Gene 231(1-2):1-13
12. Meyer DE, Chilkoti A (1999) Purification of recombinant proteins by fusion with thermally-responsive polypeptides. Nat Biotechnol 17(11):1112-1115
13. Meyer DE, Trabbic-Carlson K, Chilkoti A (2001) Protein purification by fusion with an environmentally responsive elastin-like polypeptide: effect of polypeptide length on the purification of thioredoxin. Biotechnol Prog 17(4):720-728. doi: 10.1021/bp010049o
14. Nichols NM, Benner JS, Martin DD, Evans TC Jr (2003) Zinc ion effects on individual Ssp DnaE intein splicing steps: regulating pathway progression. Biochemistry 42(18):5301-5311
15. Paulus H (2000) Protein splicing and related forms of protein autoprocessing. Annu Rev Biochem 69:447-496
16. Perler FB (2002) InBase: the intein database. Nucleic Acids Res 30(1):383-384
17. Song H, Meng Q, Liu XQ (2012) Protein trans-splicing of an atypical split intein showing structural flexibility and cross-reactivity. PLoS One 7(9):e45355. doi: 10.1371/journal.pone.0045355
18. Van Roey P, Pereira B, Li Z, Hiraga K, Belfort M, Derbyshire V (2007) Crystallographic and mutational studies of Mycobacterium tuberculosis recA mini-inteins suggest a pivotal role for a highly conserved aspartate residue. J Mol Biol 367(1):162-173. doi: 10.1016/j.jmb.2006.12.050
19. Volkmann G, Sun WC, Liu XQ (2009) Controllable protein cleavages through intein fragment complementation. Protein Sci 18(11):2393-2402. doi: 10.1002/Pro.249
20. Wood DW, Wu W, Belfort G, Derbyshire V, Belfort M (1999) A genetic system yields self-cleaving inteins for bioseparations. Nat Biotechnol 17(9):889-892
21. Wood DW, Derbyshire V, Wu W, Chartrain M, Belfort M, Belfort G (2000) Optimized single-step affinity purification with a self-cleaving intein applied to human acidic fibroblast growth factor. Biotechnol Progr 16(6):1055-1063
22. Wu H, Hu ZM, Liu XQ (1998) Protein trans-splicing by a split intein encoded in a split DnaE gene of Synechocystis sp. PCC6803. P Natl Acad Sci USA 95(16):9226-9231
23. Xu MQ, Mathys S, Evans TC, Chute IC, Wu H, Chong SR, Benner J, Liu XQ (1999) Characterization of a self-splicing mini-intein and its conversion into autocatalytic N- and C-terminal cleavage elements: facile production of protein building blocks for protein ligation. Gene 231(1-2):1-13