Elimination of in vivo cleavage between target protein and intein in the intein-mediated protein purification systems

Protein Expression and Purification

Volumn 50, Issue 1, 2006, Pages 74-81

  Cui, Chunxian ^a,c   Zhao, Wentao ^b,c   Chen, Jianglei ^c   Wang, Jianjun ^c   Li, Qianqian ^c  

^a TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^b TIANJIN UNIVERSITY   (China)

^c WAYNE STATE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Auto cleavage; Disulfide bond; High level protein expression; IMPACT expression system; Intein; pTWIN; Single cysteine mutant

Indexed keywords

APOLIPOPROTEIN E; CHAPERONE; CYSTEINE; DNA TOPOISOMERASE (ATP HYDROLYSING); INTEIN; MESD PROTEIN, MOUSE;

AFFINITY CHROMATOGRAPHY; ANIMAL; ARTICLE; BIOSYNTHESIS; CHEMICAL STRUCTURE; CHEMISTRY; GENE VECTOR; GENETICS; HUMAN; METHODOLOGY; MOUSE; PROTEIN CONFORMATION; SENSITIVITY AND SPECIFICITY; SITE DIRECTED MUTAGENESIS; X RAY CRYSTALLOGRAPHY;

ANIMALS; APOLIPOPROTEINS E; CHROMATOGRAPHY, AFFINITY; CRYSTALLOGRAPHY, X-RAY; CYSTEINE; DNA GYRASE; GENETIC VECTORS; HUMANS; INTEINS; MICE; MODELS, MOLECULAR; MOLECULAR CHAPERONES; MUTAGENESIS, SITE-DIRECTED; PROTEIN CONFORMATION; SENSITIVITY AND SPECIFICITY;

BACTERIA (MICROORGANISMS);

EID: 33750492267     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2006.05.019     Document Type: Article

References (21)

1. IMPACT™-TWIN-purification, ligation and cyclization of recombinant proteins using self-cleavable affinity tags, Version 1.0, New England BioLabs.
2. Xu M.Q., and Evans Jr. T.C. Purification of recombinant proteins from E. coli by engineered inteins. Methods Mol. Biol. 205 (2003) 43-68
3. Xu M.Q., and Evans Jr. T.C. Intein-mediated ligation and cyclization of expressed proteins. Methods 24 (2001) 257-277
4. Evans Jr. T.C., Benner J., and Xu M.Q. Semisynthesis of cytotoxic proteins using a modified protein splicing element. Protein Sci. 7 (1998) 2256-2264
5. Xu R., Ayers B., Cowburn D., and Muir T.W. Chemical ligation of folded recombinant proteins: segmental isotopic labeling of domains for NMR studies. Proc. Natl. Acad. Sci. USA 96 (1999) 388-393
6. Muir T.W., Sondhi D., and Cole P.A. Expressed protein ligation: a general method for protein engineering. Proc. Natl. Acad. Sci. USA 95 (1998) 6705-6710
7. Severinov K., and Muir T.W. Expressed protein ligation, a novel method for studying protein-protein interactions in transcription. J. Biol. Chem. 273 (1998) 16205-16209
8. Xu M.Q., and Perler F.B. The mechanism of protein splicing and its modulation by mutation. EMBO J. 15 (1996) 5146-5153
9. Klabunde T., Sharma S., Telenti A., Jacobs Jr. W.R., and Sacchettini J.C. Crystal structure of GyrA intein from Mycobacterium xenopi reveals structural basis of protein splicing. Nat. Struct. Biol. 5 (1998) 31-36
10. Anthony L., Dombkowski A., and Burgess R. Using disulfide bond engineering to study conformational changes in the β260-309 coiled-coil region of Escherichia coli RNA polymerase during 70 binding. J. Bacteriol. 184 (2002) 2634-2641
11. Dombkowski A., and Crippen G.M. Disulfide recognition in an optimized threading potential. Protein Eng. 13 (2000) 679-689
12. Nakamoto H., and Bardwell J.C.A. Catalysis of disulfide bond formation and isomerization in the Escherichia coli periplasm. Biochim. Biophys. Acta 1694 (2004) 111-119
13. Kadokura H., Katzen F., and Beckwith J. Protein disulfide bond formation in prokaryotes. Annu. Rev. Biochem. 72 (2003) 111-135
14. Weisgraber K.H. Apolipoprotein E: structure-function relationships. Adv. Protein Chem. 45 (1994) 249-302
15. Mahley R.W., and Rall Jr. S.C. Apolipoprotein E: far more than a lipid transport protein. Annu. Rev. Genomics Hum. Genet. 1 (2000) 507-537
16. Fenili D., and McLaurin J. Cholesterol and apoE: a target for Alzheimer's disease therapeutics. Curr. Drug Targets CNS Neurol. Disord. 4 (2005) 553-567
17. Weisgraber K.H., and Mahley R.W. Human apolipoprotein E: the Alzheimer's disease connection. FASEB J. 10 (1996) 1485-1494
18. Hsieh J.C., Lee L., Zhang L., Wefer S., Brown K., DeRossi C., Wines M.E., Rosenquist T., and Holdener B.C. Mesd encodes an LRP5/6 chaperone essential for specification of mouse embryonic polarity. Cell 112 (2003) 355-367
19. Herz J., and Marschang P. Coaxing the LDL receptor family into the fold. Cell 112 (2003) 289-292
20. Li Y., Chen J., Lu W., McCormick L.M., Wang J., and Bu G. Mesd binds to mature LDL-receptor-related protein-6 and antagonizes ligand binding. J. Cell Sci. 118 Pt 22 (2005) 5305-5314
21. David R., and Richter M.P. AG Beck-Sickinger. Expressed protein ligation. Method and applications. Eur. J. Biochem. 271 (2004) 663-677