False EX1 signatures caused by sample carryover during HX MS analyses

International Journal of Mass Spectrometry

Volumn 302, Issue 1-3, 2011, Pages 19-25

  Fang, Jing ^a   Rand, Kasper D ^a   Beuning, Penny J ^a   Engen, John R ^a  

^a NORTHEASTERN UNIVERSITY   (United States)

Author keywords

Deuterium; EX1 kinetics; Hydrogen exchange; Mass spectrometry; Peptide carryover

Indexed keywords

EID: 79954631167     PISSN: 13873806     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ijms.2010.06.039     Document Type: Article

References (26)

1. A. Miranker, C.V. Robinson, S.E. Radford, R.T. Aplin, and C.M. Dobson Detection of transient protein folding populations by mass spectrometry Science 262 1993 896 900 (Pubitemid 24014076)
2. C.B. Arrington, L.M. Teesch, and A.D. Robertson Defining protein ensembles with native-state NH exchange: kinetics of interconversion and cooperative units from combined NMR and MS analysis J. Mol. Biol. 285 1999 1265 1275 (Pubitemid 29054331)
3. J.R. Engen, and D.L. Smith Investigating the higher order structure of proteins. Hydrogen exchange, proteolytic fragmentation, and mass spectrometry Meth. Mol. Biol. 146 2000 95 112
4. D.D. Weis, T.E. Wales, J.R. Engen, M. Hotchko, and L.F. Ten Eyck Identification and characterization of EX1 kinetics in H/D exchange mass spectrometry by peak width analysis J. Am. Soc. Mass Spectrom. 17 2006 1498 1509 (Pubitemid 44635272)
5. S.W. Englander, and N.R. Kallenbach Hydrogen exchange and structural dynamics of proteins and nucleic acids Q. Rev. Biophys. 16 1983 521 655
6. C.B. Arrington, and A.D. Robertson Kinetics and thermodynamics of conformational equilibria in native proteins by hydrogen exchange Meth. Enzymol. 323 2000 104 124 (Pubitemid 34193275)
7. T. Sivaraman, and A.D. Robertson Kinetics of conformational fluctuations by EX1 hydrogen exchange in native proteins Meth. Mol. Biol. 168 2001 193 214
8. I.A. Kaltashov, and S.J. Eyles Studies of biomolecular conformations and conformational dynamics by mass spectrometry Mass Spectrom. Rev. 21 2002 37 71 (Pubitemid 34950940)
9. A.N. Hoofnagle, K.A. Resing, and N.G. Ahn Protein analysis by hydrogen exchange mass spectrometry Annu. Rev. Biophys. Biomol. Struct. 32 2003 1 25 (Pubitemid 37056219)
10. T.E. Wales, and J.R. Engen Partial unfolding of diverse SH3 domains on a wide timescale J. Mol. Biol. 357 2006 1592 1604
11. S.W. Englander Protein folding intermediates and pathways studied by hydrogen exchange Annu. Rev. Biophys. Biomol. Struct. 29 2000 213 238 (Pubitemid 30599593)
12. I. Kheterpal, and R. Wetzel Hydrogen/deuterium exchange mass spectrometry - a window into amyloid structure Acc. Chem. Res. 39 2006 584 593
13. Y. Wu, S. Kaveti, and J.R. Engen Extensive deuterium back-exchange in certain immobilized pepsin columns used for H/D exchange mass spectrometry Anal. Chem. 78 2006 1719 1723 (Pubitemid 43346314)
14. R. Haeckel Recommendations for definition and determination of carry-over effects J. Auto. Chem. 10 1988 181 183 (Pubitemid 19024678)
15. W. Zeng, D.G. Musson, A.L. Fisher, and A.Q. Wang A new approach for evaluating carryover and its influence on quantitation in high-performance liquid chromatography and tandem mass spectrometry assay Rapid Commun. Mass Spectrom. 20 2006 635 640 (Pubitemid 43236851)
16. J. Fang, K.D. Rand, M.C. Silva, T.E. Wales, J.R. Engen, and P.J. Beuning Conformational dynamics of the Escherichia coli DNA polymerase manager proteins UmuD and UmuD′ J. Mol. Biol. 398 2010 40 53
17. T.E. Wales, K.E. Fadgen, G.C. Gerhardt, and J.R. Engen High-speed and high-resolution UPLC separation at zero degrees Celsius Anal. Chem. 80 2008 6815 6820
18. Z. Zhang, Protein hydrogen exchange determined by mass spectrometry: a new tool for probing protein high-order structure and structural changes, Doctoral Dissertation, Purdue University, 1995, 223 pages.
19. T.E. Wales, and J.R. Engen Hydrogen exchange mass spectrometry for the analysis of protein dynamics Mass Spectrom. Rev. 25 2006 158 170
20. G. Mitulovic, C. Stingl, I. Steinmacher, O. Hudecz, J.R. Hutchins, J.M. Peters, and K. Mechtler Preventing carryover of peptides and proteins in nano LC-MS separations Anal. Chem. 81 2009 5955 5960
21. N.C. Hughes, E.Y. Wong, J. Fan, and N. Bajaj Determination of carryover and contamination for mass spectrometry-based chromatographic assays AAPS J. 9 2007 E353 360
22. P.T. Vallano, S.B. Shugarts, E.J. Woolf, and B.K. Matuszewski Elimination of autosampler carryover in a bioanalytical HPLC-MS/MS method: a case study J. Pharm. Biomed. Anal. 36 2005 1073 1078 (Pubitemid 40037828)
23. L. Wang, H. Pan, and D.L. Smith Hydrogen exchange-mass spectrometry: optimization of digestion conditions Mol. Cell Proteomics 1 2002 132 138
24. P.C. Sadek The HPLC Solvent Guide In 2002 John Wiley and Sons, Inc. New York
25. M.-I. Aguilar HPLC of Peptides and Proteins: Methods and Protocols 2004 Humana Press Inc. New Jersey
26. Y. Asakawa, C. Ozawa, K. Osada, S. Kaneko, and N. Asakawa Reduction of carry-over in column-switching HPLC/MS system with automated system washing procedure for highly sensitive direct analysis of donepezil in dog plasma J. Pharm. Biomed. Anal. 43 2007 683 690 (Pubitemid 44960583)