Mutagenesis of D80-82 and G83 residues in West Nile Virus NS2B: Effects on NS2B-NS3 activity and viral replication

Virologica Sinica

Volumn 28, Issue 1, 2013, Pages 16-23

  Jia, Fan ^a   Fan, Jingjing ^a   Zhang, Bo ^a   Yuan, Zhiming ^a  

^a WUHAN INSTITUTE OF VIROLOGY   (China)

Author keywords

NS2B; Protease; RNA replication; West Nile virus

Indexed keywords

ASPARTIC ACID; GLYCINE; NONSTRUCTURAL PROTEIN 2B; NONSTRUCTURAL PROTEIN 3; UNCLASSIFIED DRUG; VIRUS PROTEIN; VIRUS RNA;

AMINO ACID SUBSTITUTION; ARTICLE; CONTROLLED STUDY; DOWN REGULATION; ENZYME ACTIVITY; ENZYME KINETICS; GENE SEQUENCE; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; REPLICON; RNA REPLICATION; SITE DIRECTED MUTAGENESIS; VIRUS REPLICATION; VIRUS TRANSCRIPTION; WEST NILE FLAVIVIRUS;

AMINO ACID MOTIFS; AMINO ACID SEQUENCE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; RNA HELICASES; SEQUENCE ALIGNMENT; SERINE ENDOPEPTIDASES; VIRAL NONSTRUCTURAL PROTEINS; VIRUS REPLICATION; WEST NILE VIRUS;

WEST NILE VIRUS;

EID: 84873437602     PISSN: 16740769     EISSN: 1995820X     Source Type: Journal    
DOI: 10.1007/s12250-013-3276-y     Document Type: Article

References (25)

1. Chappell K J, Nall T A, Stoermer M J, Fang N X, Tyndall J D, Fairlie D P, Young P R. 2005. Site-directed Mutagenesis and Kinetic Studies of the West Nile Virus NS3 Protease Identify Key Enzyme-Substrate Interactions. J Biol Chem, 280(4):2896-2903.
2. Chappell K J, Stoermer M J, Fairlie D P, Young P R. 2008. Mutagenesis of the West Nile virus NS2B cofactor domain reveals two regions essential for protease activity. J Gen Virol, 89(Pt4):1010-1014.
3. Chambers T J, Nestorowicz A, Amberg S M, Rice C M. 1993. Mutagenesis of the yellow fever virus NS2B protein: effects on proteolytic processing, NS2B-NS3 complex formation, and viral replication. J Virol, 67(11):6797-6807.
4. Ciota A T, Lovelace A O, Ngo K A, Le A N, Maffei J G, Franke M A, Payne A F, Jones S A, Kauffman E B, Kramer L D. 2007. Cell-specific adaptation of two flaviviruses following serial passage in mosquito cell culture. Virology, 357(2):165-174.
5. D'Arcy A, Chaillet M, Schiering N, Villard F, Lim S P, Lefeuvre P, Erbel P. 2006. Purification and crystallization of dengue and West Nile virus NS2B-NS3 complexes. Acta Crystallogr Sect F Struct Biol Cryst Commun, 62(Pt2):157-162.
6. Droll D A, Krishna Murthy H M, Chambers T J. 2000. Yellow fever virus NS2B-NS3 protease: charged-to-alanine mutagenesis and deletion analysis define regions important for protease complex formation and function. Virology, 275(2):335-347.
7. Erbel P, Schiering N, D'Arcy A, Renatus M, Kroemer M, Lim S P, Yin Z, Keller T H, Vasudevan S G, Hommel U. 2006. Structural basis for the activation of flaviviral NS3 proteases from dengue and West Nile virus. Nat Struct Mol Biol, 13(4):372-373.
8. Falgout B, Miller R H, Lai C J. 1993. Deletion analysis of dengue virus type 4 nonstructural protein NS2B: identification of a domain required for NS2B-NS3 protease activity. J Virol, 67(4): 2034-2042.
9. Falgout B, Pethel M, Zhang Y M, Lai C J. 1991. Both nonstructural proteins NS2B and NS3 are required for the proteolytic processing of dengue virus nonstructural proteins. J Virol, 65(5):2467-2475.
10. Jia F, Zou G, Fan J J, Yuan Z M. 2010. Identification of palmatine as an inhibitor of West Nile virus. Arch Virol, 155(8): 1325-1329.
11. Johnston P A, Phillips J, Shun T Y, Shinde S, Lazo J S, Huryn D M, Myers M C, Ratnikov B I, Smith J W, Su Y, Dahl R, Cosford N D, Shiryaev S A, Strongin A Y. 2007. HTS Identifies Novel and Specific Uncompetitive Inhibitors of the Two-Component NS2B-NS3 Proteinase of West Nile Virus. Assay Drug Dev Technol, 5(6):737-750.
12. Leung D, Schroder K, White H, Fang N X, Stoermer M J, Abbenante G, Martin J L, Young P R, Fairlie D P. 2001. Activity of recombinant dengue 2 virus NS3 protease in the presence of a truncated NS2B co-factor, small peptide substrates, and inhibitors. J Biol Chem, 276(49):45762-45771.
13. Lin C W, Huang H D, Shiu S Y, Chen W J, Tsai M H, Huang S H, Wan L, Lin Y J. 2007. Functional determinants of NS2B for activation of Japanese encephalitis virus NS3 protease. Virus Res, 127(1):88-94.
14. Lo M K, Tilgner M, Bernard K A, Shi P Y. 2003. Functional analysis of mosquito-borne flavivirus conserved sequence elements within 3' untranslated region of West Nile virus by use of a reporting replicon that differentiates between viral translation and RNA replication. J Virol, 77(18):10004-10014.
15. Loughlin W A, Tyndall J D, Glenn M P, Fairlie D P. 2004. Beta-strand mimetics. Chem Rev, 104(12):6085-6117.
16. Mueller N H, Yon C, Ganesh V K, Padmanabhan R. 2007. Characterization of the West Nile virus protease substrate specificity and inhibitors. Int J Biochem Cell Biol, 39(3):606-614.
17. Mukhopadhyay S, Kuhn R J, Rossmann M G. 2005. A structural perspective of the flavivirus life cycle. Nat Rev Microbiol, 3(1):13-22.
18. Nall T A, Chappell K J, Stoermer M J, Fang N X, Tyndall J D, Young P R, Fairlie D P. 2004. Enzymatic Characterization and Homology Model of a Catalytically Active Recombinant West Nile Virus NS3 Protease. J Biol Chem, 279(47):48535-48542.
19. Radichev I, Shiryaev S A, Aleshin A E, Ratnikov B I, Smith J W, Liddington R C, Strongin A Y. 2008. Structure-based mutagenesis identifies important novel determinants of the NS2B cofactor of the West Nile virus two-component NS2B-NS3 proteinase. J Gen Virol, 89(Pt3):636-641.
20. Ryan M D, Monaghan S, Flint M. 1998. Virus-encoded proteinases of the Flaviviridae. J Gen Virol. 79(Pt5):947-959.
21. Shiryaev S A, Ratnikov B I, Chekanov A V, Sikora S, Rozanov D V, Godzik A, Wang J, Smith J W, Huang Z, Lindberg I, Samuel M A, Diamond M S, Strongin A Y. 2006. Cleavage targets and the D-arginine-based inhibitors of the West Nile virus NS3 processing proteinase. Biochem J, 393(Pt2):503-511.
22. Stadler K, Allison S L, Schalich J, Heinz F X. 1997. Proteolytic activation of tick-borne encephalitis virus by furin. J Virol, 71(11):8475-8481.
23. Thomas G. 2002. Furin at the cutting edge: From protein traffic to embryogenesis and disease. Nat Rev Mol Cell Biol, 3(10):753-766.
24. Tilgner M, Shi P Y. 2004. Structure and function of the 3′ terminal six nucleotides of the west nile virus genome in viral replication. J Virol, 78(15):8159-8171.
25. Tyndall J D, Nall T, Fairlie D P. 2005. Proteases universally recognize beta strands in their active sites. Chem Rev, 105(3):973-999.