Site-directed mutagenesis and kinetic studies of the West Nile virus NS3 protease identify key enzyme-substrate interactions

Journal of Biological Chemistry

Volumn 280, Issue 4, 2005, Pages 2896-2903

  Chappell, Keith J ^a   Nall, Tessa A ^a   Stoermer, Martin J ^a   Fang, Ning Xia ^a   Tyndall, Joel D A ^a,b   Fairlie, David P ^a   Young, Paul R ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

^b UNIVERSITY OF OTAGO   (New Zealand)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYST ACTIVITY; DISEASES; ENZYME INHIBITION; ENZYME KINETICS; GENETIC ENGINEERING; MUTAGENESIS;

PROTEASE INHIBITORS; VIRAL PROTEASE; WEST NILE VIRUS (WNV);

VIRUSES;

ARGININE; ASPARAGINE; ASPARTIC ACID; LYSINE; PROTEINASE INHIBITOR; RECOMBINANT ENZYME; SERINE; TYROSINE; VIRUS PROTEIN;

ARTICLE; CATALYSIS; ENZYME ACTIVE SITE; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; IN VITRO STUDY; MOLECULAR MODEL; NONHUMAN; PLASMID; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS; VIRUS REPLICATION; WEST NILE FLAVIVIRUS;

AMINO ACID SEQUENCE; CATALYTIC DOMAIN; ELECTROSTATICS; KINETICS; MODELS, GENETIC; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; PEPTIDES; PLASMIDS; PROTEIN BINDING; RECOMBINANT PROTEINS; RNA HELICASES; SEQUENCE HOMOLOGY, AMINO ACID; SERINE ENDOPEPTIDASES; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; SUBSTRATE SPECIFICITY; VIRAL NONSTRUCTURAL PROTEINS; WEST NILE VIRUS;

FLAVIVIRUS; RNA VIRUSES; WEST NILE VIRUS;

EID: 13244281716     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M409931200     Document Type: Article

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